{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T09:54:27Z","timestamp":1789206867070,"version":"build-2803163510"},"reference-count":47,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[2015,3,11]],"date-time":"2015-03-11T00:00:00Z","timestamp":1426032000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2015,3,11]],"date-time":"2015-03-11T00:00:00Z","timestamp":1426032000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Human Gene Therapy"],"published-print":{"date-parts":[[2015,5]]},"abstract":"<jats:p>\n                    Neointima formation and vascular remodeling through vascular smooth muscle cell migration and proliferation can limit the long-term success of coronary interventions, for example, in coronary artery bypass grafting (CABG).\n                    <jats:italic toggle=\"yes\">Ex vivo<\/jats:italic>\n                    gene therapy has the potential to reduce unnecessary cell proliferation and limit neointima formation in vascular pathologies. To date, the species C adenovirus serotype 5 has been commonly used for preclinical gene therapy; however, its suitability is potentially limited by relatively poor tropism for vascular cells and high levels of preexisting immunity in the population. To avoid these limitations, novel species of adenovirus are being tested; here we investigate the potential of adenovirus 49 (Ad49) for use in gene therapy. Transduction of primary human vascular cells by a range of adenovirus serotypes was assessed; Ad49 demonstrated highest transduction of both vascular smooth muscle and endothelial cells. Gene transfer with Ad49 in vascular smooth muscle and endothelial cells was possible following short exposure times (&lt;1\u2009hr) and with low MOI, which is clinically relevant.\n                    <jats:italic toggle=\"yes\">Ex vivo<\/jats:italic>\n                    delivery to surplus CABG tissue showed efficient gene transfer with Ad49, consistent with the\n                    <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                    findings. Luminal infusion of Ad49GFP into intact CABG samples\n                    <jats:italic toggle=\"yes\">ex vivo<\/jats:italic>\n                    resulted in efficient vessel transduction. In addition, no seroprevalence rates to Ad49 were observed in a Scottish cohort of patients from cardiovascular clinics, thus circumventing issues with preexisting immunity. Our results show that Ad49 has tropism for vascular cells\n                    <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                    and\n                    <jats:italic toggle=\"yes\">ex vivo<\/jats:italic>\n                    and demonstrate that Ad49 may be an improved vector for local vascular gene therapy compared with current alternatives.\n                  <\/jats:p>","DOI":"10.1089\/hum.2015.019","type":"journal-article","created":{"date-parts":[[2015,3,11]],"date-time":"2015-03-11T11:30:40Z","timestamp":1426073440000},"page":"312-319","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":31,"title":["Efficient Transduction of Primary Vascular Cells by the Rare Adenovirus Serotype 49 Vector"],"prefix":"10.1177","volume":"26","author":[{"given":"Rachel S.","family":"Dakin","sequence":"first","affiliation":[{"name":"University of Glasgow","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alan L.","family":"Parker","sequence":"additional","affiliation":[{"name":"University of Glasgow","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Delles","sequence":"additional","affiliation":[{"name":"University of Glasgow","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stuart A.","family":"Nicklin","sequence":"additional","affiliation":[{"name":"University of Glasgow","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew H.","family":"Baker","sequence":"additional","affiliation":[{"name":"University of Glasgow","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2015,3,11]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIR.0b013e31823ac046"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/mt.2011.124"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.111.022889"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/gt.2012.29"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1006\/mthe.2001.0424"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.0000109697.68832.5D"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.110.012732"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/path.1574"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.98.9.919"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1067\/mva.2001.113983"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1089\/hum.1998.9.6-867"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.101.3.296"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.gt.3301431"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/mt.2008.188"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.72.5.1132"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.82.12.1243"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1038\/gt.2013.44"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2008.10.020"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.174.11.7179"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.9.4426-4433.2005"},{"key":"e_1_3_3_22_2","first-page":"2345","article-title":"Inhibitory effects of human sera on adenovirus-mediated gene transfer into rat liver","volume":"18","author":"Kuriyama S","year":"1998","unstructured":"Kuriyama S, , Tominaga K, , Kikukawa M, et al. Inhibitory effects of human sera on adenovirus-mediated gene transfer into rat liver. 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