{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,25]],"date-time":"2026-05-25T08:02:24Z","timestamp":1779696144295,"version":"3.53.1"},"reference-count":46,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2015,12,28]],"date-time":"2015-12-28T00:00:00Z","timestamp":1451260800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Human Gene Therapy"],"published-print":{"date-parts":[[2016,1]]},"abstract":"<jats:p>\n                    Sustained expression of the\n                    <jats:italic toggle=\"yes\">CFTR<\/jats:italic>\n                    gene is a major challenge to gene therapy with either viral or nonviral vectors with immune response to vector and transgene products. One strategy to achieve sustained\n                    <jats:italic toggle=\"yes\">CFTR<\/jats:italic>\n                    expression is to modulate the host immune system through transient immunosuppression. In this study, we examined cyclophosphamide (cytoxan), dexamethasone (Dex), and a combination of cyclosporin, methylprednisolone, and azathioprine (combination) for their effects on long-term expression of the human\n                    <jats:italic toggle=\"yes\">CFTR<\/jats:italic>\n                    delivered with helper-dependent adenoviral vectors in mouse airways. We found that cyclophosphamide significantly enhanced long-term expression of the transgenic human\n                    <jats:italic toggle=\"yes\">CFTR<\/jats:italic>\n                    and the reporter gene\n                    <jats:italic toggle=\"yes\">LacZ<\/jats:italic>\n                    by reducing host immune responses. Dex administration greatly reduced neutralizing antibody production but had no effect on transgene expression. Treatment with a combination of cyclosporin A, azathioprine, and methylprednisolone affected neither\n                    <jats:italic toggle=\"yes\">CFTR<\/jats:italic>\n                    gene expression nor inflammation. Our data suggest that transient immunosuppression might be a strategy to improve sustained expression in gene therapy.\n                  <\/jats:p>","DOI":"10.1089\/hum.2015.108","type":"journal-article","created":{"date-parts":[[2015,12,29]],"date-time":"2015-12-29T04:43:38Z","timestamp":1451364218000},"page":"83-91","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":3,"title":["Long-Term Expression of the Human\n                    <i>CFTR<\/i>\n                    Gene in Mouse Airway via Helper-Dependent Adenoviral Vector Delivery and Transient Immunosuppression"],"prefix":"10.1177","volume":"27","author":[{"given":"Huibi","family":"Cao","sequence":"first","affiliation":[{"name":"University of Toronto","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Wu","sequence":"additional","affiliation":[{"name":"University of Toronto","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cathleen","family":"Duan","sequence":"additional","affiliation":[{"name":"University of Toronto","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai","family":"Du","sequence":"additional","affiliation":[{"name":"University of Toronto","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chan Mi","family":"Lee","sequence":"additional","affiliation":[{"name":"University of Toronto","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Herman","family":"Yeger","sequence":"additional","affiliation":[{"name":"University of Toronto","place":["Canada"]},{"name":"University of Toronto","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jim","family":"Hu","sequence":"additional","affiliation":[{"name":"University of Toronto","place":["Canada"]},{"name":"University of Toronto","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2015,12,28]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/dds345"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.7861\/clinmedicine.14-1-76"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1517\/14728214.12.2.329"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1258\/jrsm.2012.12s002"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1105185"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1409547"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S2213-2600(15)00245-3"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.gendis.2014.06.001"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.mt.6300002"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng0994-42"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199509283331302"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(93)80063-K"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI7935"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1089\/vim.2004.17.327"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.24.13565"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1525-0016(03)00059-5"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1038\/mtna.2013.55"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1002\/jgm.797"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2436478100"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2249.2003.02124.x"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmed.2008.11.005"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.74.7.3345-3352.2000"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.75.10.4792-4801.2001"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1038\/mt.2012.111"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1038\/gt.2010.125"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.gendis.2014.09.002"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.gt.3302712"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.cr.7290275"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1053-2498(00)00166-2"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-2277.1991.tb01942.x"},{"key":"e_1_3_3_32_2","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1111\/j.1432-2277.1991.tb01969.x","article-title":"Renal allograft immunosuppression. 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