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Here we investigated the role of the IL-17\/IL-17 receptor A (IL-17RA) axis in regulating melanoma progression and evaluated the therapeutic potential of blocking the IL-17\/IL-17RA pathway. First, recombinant mouse IL-17 (\u03b3mIL-17) treatment significantly increased proliferation of mouse B16F10 cells and human A375 and A2058 cells. Silencing IL-17RA by small hairpin RNA (shRNA) in B16F10 cells reduced the \u03b3mIL-17\u2013elicited cell proliferation, migration, and invasion, and significantly reduced vascular endothelial growth factor and matrix metalloproteinase production. Remarkably, knockdown of IL-17RA led to a significantly decreased capability of B16F10 cells to form tumors\n                    <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                    , similar to that in IL-17-deficient mice. Finally, local application of an adenovirus delivering a shRNA against IL-17RA mRNA not only significantly suppressed tumor development, but also enhanced antitumor immunity by increasing the interferon \u03b3\u2013expressing T cells and not T regulatory cells. Our results highlight the critical role of the IL-17\/IL-17RA pathway in tumor progression and imply that targeting IL-17RA represents a promising therapeutic strategy.\n                  <\/jats:p>","DOI":"10.1089\/hum.2018.104","type":"journal-article","created":{"date-parts":[[2018,8,4]],"date-time":"2018-08-04T05:19:40Z","timestamp":1533359980000},"page":"273-285","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":25,"title":["Locally Targeting the IL-17\/IL-17RA Axis Reduced Tumor Growth in a Murine B16F10 Melanoma Model"],"prefix":"10.1177","volume":"30","author":[{"given":"Ya-Shan","family":"Chen","sequence":"first","affiliation":[{"name":"Department and Graduate Institute of Medical Biotechnology and Laboratory Science, College of Medicine, Chang Gung University, Taoyuan, Taiwan, ROC."},{"name":"Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan, Taiwan, ROC."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tse-Hung","family":"Huang","sequence":"additional","affiliation":[{"name":"School of Traditional Chinese Medicine, Chang Gung University, Taoyuan, Taiwan, ROC."},{"name":"Department of Traditional Chinese Medicine, Chang Gung Memorial Hospital, Keelung, Taiwan, ROC."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chao-Lin","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Engineering, Chang Gung University, Taoyuan, Taiwan, ROC."},{"name":"Department of Chemical Engineering, Ming Chi University of Technology, New Taipei, Taiwan, ROC."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui-Shan","family":"Chen","sequence":"additional","affiliation":[{"name":"Department and Graduate Institute of Medical Biotechnology and Laboratory Science, College of Medicine, Chang Gung University, Taoyuan, Taiwan, ROC."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meng-Hua","family":"Lee","sequence":"additional","affiliation":[{"name":"Department and Graduate Institute of Medical Biotechnology and Laboratory Science, College of Medicine, Chang Gung University, Taoyuan, Taiwan, ROC."},{"name":"Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan, Taiwan, ROC."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hsin-Wei","family":"Chen","sequence":"additional","affiliation":[{"name":"National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Miaoli, Taiwan, ROC."},{"name":"Graduate Institute of Biomedical Sciences, China Medical University, Taichung, Taiwan, ROC."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chia-Rui","family":"Shen","sequence":"additional","affiliation":[{"name":"Department and Graduate Institute of Medical Biotechnology and Laboratory Science, College of Medicine, Chang Gung University, Taoyuan, Taiwan, ROC."},{"name":"Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan, Taiwan, ROC."},{"name":"Department of Ophthalmology, Lin-Kou Chang Gung Memorial Hospital, Taoyuan, Taiwan, ROC."},{"name":"Chang Gung Immunology Consortium, Chang Gung Memorial Hospital and Chang Gung University, Taoyuan, Taiwan, ROC."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2019,3,18]]},"reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(00)04046-0"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2013.08.034"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2013.01.024"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature09707"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10555-012-9415-3"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2008.271"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrc1926"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.it.2010.04.002"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.178.11.6730"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0710686105"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.lungcan.2009.11.013"},{"key":"e_1_3_4_13_2","first-page":"77","article-title":"The role of Tc17 in cancer immunity and clinical associations","volume":"25","author":"Chen YS","year":"2013","unstructured":"Chen YS, , Liu CL, , Lee MH, et al. 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