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As the interaction of vaccines with dendritic cells (DCs) shapes the immunological response, we compared the interaction of a range of virus-based vectors and virus-like particles with DCs in a murine model of systemic administration and transcriptome analyses of splenic DCs. The transcriptome profiles of DCs separated the vaccine vectors into two distinct groups characterized by high- and low-magnitude differential gene expression, which strongly correlated with (1) the surface expression of costimulatory molecules CD40, CD83, and CD86 on DCs, and (2) antigen-specific T-cell responses. Pathway analysis using PANOGA (Pathway and Network-Oriented GWAS Analysis) revealed that the JAK\/STAT pathway was significantly activated by both groups of vaccines. In contrast, the oxidative phosphorylation pathway was significantly downregulated only by the high-magnitude DC-stimulating vectors. A gene signature including exclusively chemokine-, cytokine-, and receptor-related genes revealed a vector-specific pattern. Overall, this\n                    <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                    DC stimulation model demonstrated a strong relationship between the levels of induced DC maturation and the intensity of T-cell-specific immune responses with a distinct cytokine\/chemokine profile, metabolic shifting, and cell surface expression of maturation markers. It could represent an important tool for vaccine design.\n                  <\/jats:p>","DOI":"10.1089\/hum.2018.161","type":"journal-article","created":{"date-parts":[[2018,10,23]],"date-time":"2018-10-23T17:16:16Z","timestamp":1540314976000},"page":"429-445","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":2,"title":["Comparison of Dendritic Cell Activation by Virus-Based Vaccine Delivery Vectors Emphasizes the Transcriptional Downregulation of the Oxidative Phosphorylation Pathway"],"prefix":"10.1177","volume":"30","author":[{"given":"Eliza","family":"Tsitoura","sequence":"first","affiliation":[{"name":"Molecular Virology Laboratory, Hellenic Pasteur Institute, Athens, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dorothea","family":"Kazazi","sequence":"additional","affiliation":[{"name":"Molecular Virology Laboratory, Hellenic Pasteur Institute, Athens, 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