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SXC is performed by mixing an unpurified cell culture supernatant containing AAV particles with polyethylene glycol (PEG) and feeding the mixture onto a chromatography filter unit. The purified AAV particles are recovered by flushing the unit with a solution lacking PEG. SXC is an inexpensive single-use method that permits to concentrate, purify, and re-buffer AAV particles with yields &gt;95% and &gt;80% impurity clearance. SXC could theoretically be employed at industrial scales with units of nearly 20\u2009m\n                    <jats:sup>2<\/jats:sup>\n                    .\n                  <\/jats:p>","DOI":"10.1089\/hum.2019.284","type":"journal-article","created":{"date-parts":[[2021,2,7]],"date-time":"2021-02-07T21:23:59Z","timestamp":1612733039000},"page":"959-974","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":39,"title":["Single-Use Capture Purification of Adeno-Associated Viral Gene Transfer Vectors by Membrane-Based Steric Exclusion Chromatography"],"prefix":"10.1177","volume":"32","author":[{"given":"Pavel","family":"Marichal-Gallardo","sequence":"first","affiliation":[{"name":"Bioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Magdeburg, 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