{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,6]],"date-time":"2026-06-06T01:33:45Z","timestamp":1780709625331,"version":"3.54.1"},"reference-count":74,"publisher":"SAGE Publications","issue":"13-14","license":[{"start":{"date-parts":[[2021,7,1]],"date-time":"2021-07-01T00:00:00Z","timestamp":1625097600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2021,7,1]],"date-time":"2021-07-01T00:00:00Z","timestamp":1625097600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Human Gene Therapy"],"published-print":{"date-parts":[[2021,7]]},"abstract":"<jats:p>\n                    To understand\n                    <jats:italic toggle=\"yes\">RS1<\/jats:italic>\n                    gene interaction networks in the X-linked retinoschisis (XLRS) mouse retina (\n                    <jats:italic toggle=\"yes\">Rs1<\/jats:italic>\n                    <jats:sup>\u2212\/y<\/jats:sup>\n                    ), we analyzed the transcriptome by RNA sequencing before and after\n                    <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                    expression of exogenous retinoschisin (\n                    <jats:italic toggle=\"yes\">RS1<\/jats:italic>\n                    ) gene delivered by AAV8. RS1 is a secreted cell adhesion protein that is critical for maintaining structural lamination and synaptic integrity of the neural retina.\n                    <jats:italic toggle=\"yes\">RS1<\/jats:italic>\n                    loss-of-function mutations cause XLRS disease in young boys and men, with splitting (\u201cschisis\u201d) of retinal layers and synaptic dysfunction that cause progressive vision loss with age. Analysis of differential gene expression profiles and pathway enrichment analysis of\n                    <jats:italic toggle=\"yes\">Rs1<\/jats:italic>\n                    -KO (\n                    <jats:italic toggle=\"yes\">Rs1<\/jats:italic>\n                    <jats:sup>\u2212\/y<\/jats:sup>\n                    ) retina identified cell surface receptor signaling and positive regulation of cell adhesion as potential\n                    <jats:italic toggle=\"yes\">RS1<\/jats:italic>\n                    gene interaction networks. Most importantly, it also showed massive dysregulation of immune response genes at early age, with characteristics of a microglia-driven proinflammatory state. Delivery of AAV8-\n                    <jats:italic toggle=\"yes\">RS1<\/jats:italic>\n                    primed the\n                    <jats:italic toggle=\"yes\">Rs1<\/jats:italic>\n                    -KO retina toward structural and functional recovery. The disease transcriptome transitioned toward a recovery phase with upregulation of genes implicated in wound healing, anatomical structure (camera type eye) development, metabolic pathways, and collagen IV networks that provide mechanical stability to basement membrane. AAV8-\n                    <jats:italic toggle=\"yes\">RS1<\/jats:italic>\n                    expression also attenuated the microglia gene signatures to low levels toward immune quiescence. This study is among the first to identify\n                    <jats:italic toggle=\"yes\">RS1<\/jats:italic>\n                    gene interaction networks that underlie retinal structural and functional recovery after\n                    <jats:italic toggle=\"yes\">RS1<\/jats:italic>\n                    gene therapy. Significantly, it also shows that providing wild-type\n                    <jats:italic toggle=\"yes\">RS1<\/jats:italic>\n                    gene function caused the retina immune status to transition from a degenerative inflammatory phenotype toward immune quiescence, even though the transgene is not directly linked to microglia function. This study indicates that inhibition of microglial proinflammatory responses is an integral part of therapeutic rescue in XLRS gene therapy, and gene therapy might realize its full potential if delivered before microglia activation and photoreceptor cell death. Clinical Trials. gov Identifier NTC 02317887.\n                  <\/jats:p>","DOI":"10.1089\/hum.2020.213","type":"journal-article","created":{"date-parts":[[2020,10,6]],"date-time":"2020-10-06T03:43:41Z","timestamp":1601955821000},"page":"667-681","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":28,"title":["Genetic Rescue of X-Linked Retinoschisis Mouse (\n                    <i>Rs1<\/i>\n                    <sup>\u2212\/y<\/sup>\n                    ) Retina Induces Quiescence of the Retinal Microglial Inflammatory State Following AAV8-\n                    <i>RS1<\/i>\n                    Gene Transfer and Identifies Gene Networks Underlying Retinal Recovery"],"prefix":"10.1177","volume":"32","author":[{"given":"Camasamudram","family":"Vijayasarathy","sequence":"first","affiliation":[{"name":"Section for Translational Research in Retinal and Macular Degeneration,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong","family":"Zeng","sequence":"additional","affiliation":[{"name":"Section for Translational Research in Retinal and Macular Degeneration,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew J.","family":"Brooks","sequence":"additional","affiliation":[{"name":"Neurobiology-Neurodegeneration and Repair Laboratory, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert N.","family":"Fariss","sequence":"additional","affiliation":[{"name":"Biological Imaging Core, National Eye Institute, National Institutes of Health, Bethesda, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul A.","family":"Sieving","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Center for Ocular Regenerative Therapy, School of Medicine, University of California at Davis, Sacramento, CA, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2021,7,16]]},"reference":[{"key":"e_1_3_5_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/s003359900991"},{"key":"e_1_3_5_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng1097-164"},{"key":"e_1_3_5_4_2","first-page":"169","article-title":"Congenital X-linked retinoschisis: an updated clinical review","volume":"7","author":"Rao P","year":"2018","unstructured":"Rao P, , Dedania VS, , Drenser KA. Congenital X-linked retinoschisis: an updated clinical review. 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Expression of X-linked retinoschisis protein RS1 in photoreceptor and bipolar cells. Invest Ophthalmol Vis Sci, 2001; 42:816\u2013825.","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"e_1_3_5_18_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201806148"},{"key":"e_1_3_5_19_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1519048113"},{"key":"e_1_3_5_20_2","first-page":"5311","article-title":"Structural analysis of X-linked retinoschisis mutations reveals distinct classes which differentially effect retinoschisin function","volume":"25","author":"Ramsay EP","year":"2016","unstructured":"Ramsay EP, , Collins RF, , Owens TW, et al. Structural analysis of X-linked retinoschisis mutations reveals distinct classes which differentially effect retinoschisin function. 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