{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T19:58:41Z","timestamp":1782417521710,"version":"3.54.5"},"reference-count":32,"publisher":"SAGE Publications","issue":"9-10","license":[{"start":{"date-parts":[[2022,5,1]],"date-time":"2022-05-01T00:00:00Z","timestamp":1651363200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Human Gene Therapy"],"published-print":{"date-parts":[[2022,5]]},"abstract":"<jats:p>Pompe disease is an autosomal recessive lysosomal storage disorder caused by deficiency of acid \u03b1-glucosidase (GAA), resulting in skeletal muscle weakness and cardiomyopathy that progresses despite currently available therapy in some patients. The development of gene therapy with adeno-associated virus (AAV) vectors revealed a sex-dependent decrease in efficacy in female mice with Pompe disease. This study evaluated the effect of testosterone on gene therapy with an AAV2\/8 vector containing a liver-specific promoter to drive expression of GAA (AAV2\/8-LSPhGAA) in female GAA-knockout (KO) mice that were implanted with pellets containing testosterone propionate before vector administration. Six weeks after treatment, neuromuscular function and muscle strength were improved as demonstrated by increased Rotarod and wirehang latency for female mice treated with testosterone and vector, in comparison with vector alone. Biochemical correction improved after the addition of testosterone as demonstrated by increased GAA activity and decreased glycogen content in the skeletal muscles of female mice treated with testosterone and vector, in comparison with vector alone. An alternative androgen, oxandrolone, was evaluated similarly to reveal increased GAA in the diaphragm and extensor digitorum longus of female GAA-KO mice after oxandrolone administration; however, glycogen content was unchanged by oxandrolone treatment. The efficacy of androgen hormone treatment in females correlated with increased mannose-6-phosphate receptor in skeletal muscle. These data confirmed the benefits of brief treatment with an androgen hormone in mice with Pompe disease during gene therapy.<\/jats:p>","DOI":"10.1089\/hum.2021.218","type":"journal-article","created":{"date-parts":[[2022,1,27]],"date-time":"2022-01-27T02:59:16Z","timestamp":1643252356000},"page":"479-491","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":7,"title":["Efficacious Androgen Hormone Administration in Combination with Adeno-Associated Virus Vector-Mediated Gene Therapy in Female Mice with Pompe Disease"],"prefix":"10.1177","volume":"33","author":[{"given":"Sang-oh","family":"Han","sequence":"first","affiliation":[{"name":"Department of Pediatrics, Duke University School of Medicine, Durham, North Carolina, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9636-1235","authenticated-orcid":false,"given":"Dorothy","family":"Gheorghiu","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Duke University School of Medicine, Durham, North Carolina, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alex","family":"Chang","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Duke University School of Medicine, Durham, North Carolina, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sweet Hope","family":"Mapatano","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Duke University School of Medicine, Durham, North Carolina, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Songtao","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Duke University School of Medicine, Durham, North Carolina, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth","family":"Brooks","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Duke University School of Medicine, Durham, North Carolina, USA."},{"name":"Department of Laboratory Animal Resources, Duke University, Durham, North Carolina, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dwight","family":"Koeberl","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Duke University School of Medicine, Durham, North Carolina, USA."},{"name":"Department of Molecular Genetics and Microbiology, Duke University, Durham, North Carolina, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2022,5,16]]},"reference":[{"key":"e_1_3_1_2_2","volume-title":"The Metabolic and Molecular Basis for Inherited Disease, 8th ed","author":"Hirschhorn R","unstructured":"Hirschhorn R, , Reuser AJJ. 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Salmeterol with liver depot gene therapy enhances the skeletal muscle response in murine Pompe disease. Hum Gene Ther 2019.","journal-title":"Hum Gene Ther"},{"key":"e_1_3_1_26_2","doi-asserted-by":"publisher","DOI":"10.1086\/522236"},{"key":"e_1_3_1_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymthe.2004.09.017"},{"key":"e_1_3_1_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/B978-008055232-3.62339-6"},{"key":"e_1_3_1_29_2","doi-asserted-by":"publisher","DOI":"10.1080\/15548627.2015.1009779"},{"key":"e_1_3_1_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymgme.2011.11.005"},{"key":"e_1_3_1_31_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa0909859"},{"key":"e_1_3_1_32_2","first-page":"CD009749","article-title":"Androgens (dehydroepiandrosterone or testosterone) for women undergoing assisted reproduction","author":"Nagels HE","year":"2015","unstructured":"Nagels HE, , Rishworth JR, , Siristatidis CS, et al. 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