{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T02:47:54Z","timestamp":1792464474500,"version":"4.5.0"},"reference-count":36,"publisher":"SAGE Publications","issue":"19-20","license":[{"start":{"date-parts":[[2022,10,1]],"date-time":"2022-10-01T00:00:00Z","timestamp":1664582400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Human Gene Therapy"],"published-print":{"date-parts":[[2022,10]]},"abstract":"<jats:p>\n                    Natural airway defenses currently impede the efficacy of viral vector-mediated airway gene therapy. Conditioning airways before vector delivery can disrupt these barriers, improving viral vector access to target receptors and airway stem cells. This study aimed to assess and quantify the\n                    <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                    histological and gene transfer effects of physical perturbation devices to identify effective conditioning approaches. A range of flexible wire baskets with varying configurations, a Brush, biopsy forceps, and a balloon catheter were examined. We first evaluated the histological effects of physical perturbation devices in rat tracheas that were excised 10\u2009min after conditioning. Based on the histological findings, a selection of devices was used to condition rat tracheas\n                    <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                    before delivering a lentiviral vector containing the\n                    <jats:italic toggle=\"yes\">LacZ<\/jats:italic>\n                    reporter gene. After 7 days, excised tracheas were X-gal processed and examined\n                    <jats:italic toggle=\"yes\">en face<\/jats:italic>\n                    to quantify the area of LacZ staining. Histological observations 10\u2009min after conditioning found that physical perturbation dislodged cells from the basement membrane to varying degrees, with some producing significant levels of epithelial cell removal. When a subset of devices was assessed for their ability to enhance gene transfer, only the NGage\n                    <jats:sup>\u00ae<\/jats:sup>\n                    wire basket (Cook Medical) produced a significant increase in the proportion of X-gal-stained area when compared with unconditioned tracheas (eightfold,\n                    <jats:italic toggle=\"yes\">p<\/jats:italic>\n                    \u2009=\u20090.00025). These results suggest that a range of factors contribute to perturbation-enhanced gene transfer. Overall, this study supports existing evidence that physical perturbation can assist airway gene transfer and will help to identify the characteristics of an effective device for airway gene therapy.\n                  <\/jats:p>","DOI":"10.1089\/hum.2022.086","type":"journal-article","created":{"date-parts":[[2022,8,3]],"date-time":"2022-08-03T05:33:06Z","timestamp":1659504786000},"page":"1062-1072","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":2,"title":["Comparison of Physical Perturbation Devices for Enhancing Lentiviral Vector-Mediated Gene Transfer to the Airway Epithelium"],"prefix":"10.1177","volume":"33","author":[{"given":"Victoria","family":"Drysdale","sequence":"first","affiliation":[{"name":"Adelaide Medical School, University of Adelaide, Adelaide, Australia"},{"name":"Robinson Research Institute, University of Adelaide, Adelaide, Australia"},{"name":"Respiratory and Sleep Medicine, Women's and Children's Hospital, Adelaide, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia","family":"Cmielewski","sequence":"additional","affiliation":[{"name":"Adelaide Medical School, University of Adelaide, Adelaide, Australia"},{"name":"Robinson Research Institute, University of Adelaide, Adelaide, Australia"},{"name":"Respiratory and Sleep Medicine, Women's and Children's Hospital, Adelaide, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5320-7756","authenticated-orcid":false,"given":"Martin","family":"Donnelley","sequence":"additional","affiliation":[{"name":"Adelaide Medical School, University of Adelaide, Adelaide, Australia"},{"name":"Robinson Research Institute, University of Adelaide, Adelaide, Australia"},{"name":"Respiratory and Sleep Medicine, Women's and Children's Hospital, Adelaide, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicole","family":"Reyne","sequence":"additional","affiliation":[{"name":"Adelaide Medical School, University of Adelaide, Adelaide, Australia"},{"name":"Robinson Research Institute, University of Adelaide, Adelaide, Australia"},{"name":"Respiratory and Sleep Medicine, Women's and Children's Hospital, Adelaide, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1746-3290","authenticated-orcid":false,"given":"David","family":"Parsons","sequence":"additional","affiliation":[{"name":"Adelaide Medical School, University of Adelaide, Adelaide, Australia"},{"name":"Robinson Research Institute, University of Adelaide, Adelaide, Australia"},{"name":"Respiratory and Sleep Medicine, Women's and Children's Hospital, Adelaide, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6045-3998","authenticated-orcid":false,"given":"Alexandra","family":"McCarron","sequence":"additional","affiliation":[{"name":"Adelaide Medical School, University of Adelaide, Adelaide, Australia"},{"name":"Robinson Research Institute, University of Adelaide, Adelaide, Australia"},{"name":"Respiratory and Sleep Medicine, Women's and Children's Hospital, Adelaide, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2022,10,17]]},"reference":[{"key":"e_1_3_6_2_2","doi-asserted-by":"publisher","DOI":"10.1080\/01902148.2017.1395931"},{"issue":"14","key":"e_1_3_6_3_2","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1186\/1471-2466-14-183","article-title":"Transduction of ferret airway epithelia using a pre-treatment and lentiviral gene vector","volume":"21","author":"Cmielewski P","year":"2014","unstructured":"Cmielewski P, , Farrow N, , Donnelley M, et al. Transduction of ferret airway epithelia using a pre-treatment and lentiviral gene vector. BMC Pulm Med, 2014; 21(14):183; doi: 10.1186\/1471-2466-14-183.","journal-title":"BMC Pulm Med"},{"key":"e_1_3_6_4_2","doi-asserted-by":"publisher","DOI":"10.1089\/hum.2020.267"},{"key":"e_1_3_6_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/jgm.1481"},{"key":"e_1_3_6_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2016.05.031"},{"key":"e_1_3_6_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/mt.2014.89"},{"key":"e_1_3_6_8_2","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0019919","volume":"6","author":"Hida K","year":"2011","unstructured":"Hida K, , Lai SK, , Suk JS, et al. Common gene therapy viral vectors do not efficiently penetrate sputum from cystic fibrosis patients. 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