{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T16:51:57Z","timestamp":1781801517712,"version":"3.54.5"},"reference-count":32,"publisher":"SAGE Publications","issue":"7-8","license":[{"start":{"date-parts":[[2023,4,1]],"date-time":"2023-04-01T00:00:00Z","timestamp":1680307200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Human Gene Therapy"],"published-print":{"date-parts":[[2023,4]]},"abstract":"<jats:p>\n                    Transplantation, the gold standard intervention for organ failure, is a clinical field that is ripe for applications of gene therapy. One of the major challenges in applying gene therapy to this field is the need for a method that achieves consistent and robust gene delivery to allografts. Normothermic\n                    <jats:italic toggle=\"yes\">ex vivo<\/jats:italic>\n                    perfusion is a growing organ preservation method and a device for cardiac preservation was recently approved by the Food and Drug Administration (FDA) (Organ Care System, OCS\u2122; TransMedics, Inc., Andover, MA); this device maintains donor hearts in a near physiologic state while they are transported from the donor to the recipient. This study describes the administration of recombinant adeno-associated viral vectors (rAAVs) during\n                    <jats:italic toggle=\"yes\">ex vivo<\/jats:italic>\n                    normothermic perfusion for the delivery of transgenes to porcine cardiac allografts. We utilized a myocardial-enhanced AAV3b variant, SASTG, assessing its transduction efficiency in the OCS perfusate relative to other AAV serotypes. We describe the use of normothermic\n                    <jats:italic toggle=\"yes\">ex vivo<\/jats:italic>\n                    perfusion to deliver SASTG carrying the Firefly Luciferase transgene to porcine donor hearts in four heterotopic transplant procedures. Durable and dose-dependent transgene expression was achieved in the allografts in 30 days, with no evidence of off-target transgene expression. This study demonstrates the feasibility and efficiency of delivering genes to a large animal allograft utilizing AAV vectors during\n                    <jats:italic toggle=\"yes\">ex vivo<\/jats:italic>\n                    perfusion. These findings support the idea of gene therapy interventions to enhance transplantation outcomes.\n                  <\/jats:p>","DOI":"10.1089\/hum.2022.241","type":"journal-article","created":{"date-parts":[[2023,3,17]],"date-time":"2023-03-17T02:36:07Z","timestamp":1679020567000},"page":"303-313","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":17,"title":["<i>Ex Vivo<\/i>\n                    Gene Delivery to Porcine Cardiac Allografts Using a Myocardial-Enhanced Adeno-Associated Viral Vector"],"prefix":"10.1177","volume":"34","author":[{"given":"Michelle","family":"Mendiola Pla","sequence":"first","affiliation":[{"name":"Division of Cardiothoracic Surgery, Department of Surgery, Duke University Medical Center, Durham, North Carolina, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuting","family":"Chiang","sequence":"additional","affiliation":[{"name":"Division of Cardiothoracic Surgery, Department of Surgery, Columbia University Medical Center, New York City, New York, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio","family":"Roki","sequence":"additional","affiliation":[{"name":"Division of Surgical Sciences, Department of Surgery, Duke University Medical Center, Durham, North Carolina, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunbo","family":"Wang","sequence":"additional","affiliation":[{"name":"Division of Surgical Sciences, Department of Surgery, Duke University Medical Center, Durham, North Carolina, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franklin H.","family":"Lee","sequence":"additional","affiliation":[{"name":"Division of Surgical Sciences, Department of Surgery, Duke University Medical Center, Durham, North Carolina, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew F.","family":"Smith","sequence":"additional","affiliation":[{"name":"Division of Surgical Sciences, Department of Surgery, Duke University Medical Center, Durham, North Carolina, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryan T.","family":"Gross","sequence":"additional","affiliation":[{"name":"Division of Surgical Sciences, Department of Surgery, Duke University Medical Center, Durham, North Carolina, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun-Neng","family":"Roan","sequence":"additional","affiliation":[{"name":"Division of Cardiovascular Surgery, Department of Surgery, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, Taiwan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muath","family":"Bishawi","sequence":"additional","affiliation":[{"name":"Division of Cardiothoracic Surgery, Department of Surgery, Duke University Medical Center, Durham, North Carolina, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amy","family":"Evans","sequence":"additional","affiliation":[{"name":"Perfusion Services, Duke University Medical Center, Durham, North Carolina, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lynden E.","family":"Gault","sequence":"additional","affiliation":[{"name":"Gift of Hope Organ and Tissue Donor Network, Itasca, Illinois, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sam","family":"Ho","sequence":"additional","affiliation":[{"name":"Gift of Hope Organ and Tissue Donor Network, Itasca, Illinois, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolyn","family":"Glass","sequence":"additional","affiliation":[{"name":"Department of Pathology, Duke University Medical Center, Durham, North Carolina, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacob N.","family":"Schroder","sequence":"additional","affiliation":[{"name":"Division of Cardiothoracic Surgery, Department of Surgery, Duke University Medical Center, Durham, North Carolina, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul","family":"Lezberg","sequence":"additional","affiliation":[{"name":"TransMedics, Inc., Andover, Massachusetts, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carmelo A.","family":"Milano","sequence":"additional","affiliation":[{"name":"Division of Cardiothoracic Surgery, Department of Surgery, Duke University Medical Center, Durham, North Carolina, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dawn E.","family":"Bowles","sequence":"additional","affiliation":[{"name":"Division of Surgical Sciences, Department of Surgery, Duke University Medical Center, Durham, North Carolina, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2023,4,17]]},"reference":[{"key":"e_1_3_6_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/ppul.24203"},{"key":"e_1_3_6_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ophtha.2019.06.017"},{"key":"e_1_3_6_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymthe.2020.12.007"},{"key":"e_1_3_6_5_2","doi-asserted-by":"crossref","unstructured":"Mendiola Pla M Bowles DE. 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