{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T05:49:08Z","timestamp":1777700948650,"version":"3.51.4"},"reference-count":37,"publisher":"SAGE Publications","issue":"8","license":[{"start":{"date-parts":[[2011,8,1]],"date-time":"2011-08-01T00:00:00Z","timestamp":1312156800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Journal of Interferon &amp; Cytokine Research"],"published-print":{"date-parts":[[2011,8]]},"abstract":"<jats:p>The human interferon (IFN)-inducible IFI16 protein is a member of the 200-amino acid repeat family encoded by the HIN-200 genes. Forced IFI16 expression in normal human endothelial cells (ECs) inhibits cell growth and tube morphogenesis of ECs through the triggering of apoptosis by caspase-2 and caspase-3 via nuclear factor-\u03baB (NF-\u03baB) complex activation. Accumulating evidence suggests that tumor-derived ECs (TECs) possess a distinct and unique phenotype compared with normal ECs, and they may be able to acquire resistance to antiangiogenic agents such as IFNs. However, few functional studies are available on cultured TEC. In the present study, we have demonstrated that TEC obtained from tumors of various histological origin, namely kidney (Eck25), breast (B-TEC), and head and neck (HN4), continued to proliferate and generate microtubules on Matrigel following IFI16 overexpression. In contrast to normal ECs, they were resistant to apoptosis triggered by caspase-2 and caspase-3 activation via the NF-\u03baB complex. At the molecular level, when overexpressed in TEC, IFI16 appeared unable to regulate NF-\u03baB activity and lead to caspase activation. Altogether, these results indicate that TECs display abnormal responses, in terms of survival and angiogenic properties, to an antiproliferative and antiangiogenic IFN-inducible gene such as IFI16.<\/jats:p>","DOI":"10.1089\/jir.2011.0001","type":"journal-article","created":{"date-parts":[[2011,4,13]],"date-time":"2011-04-13T16:41:13Z","timestamp":1302712873000},"page":"609-618","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":5,"title":["Tumor-Derived Endothelial Cells Evade Apoptotic Activity of the Interferon-Inducible IFI16 Gene"],"prefix":"10.1177","volume":"31","author":[{"given":"Francesca","family":"Gugliesi","sequence":"first","affiliation":[{"name":"University of Turin","place":["Italy"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Valentina","family":"Dell'Oste","sequence":"additional","affiliation":[{"name":"University of Turin","place":["Italy"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marco","family":"De Andrea","sequence":"additional","affiliation":[{"name":"University of Turin","place":["Italy"]},{"name":"University of Eastern Piedmont \u201cA. Avogadro,\u201d Novara","place":["Italy"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rossella","family":"Baggetta","sequence":"additional","affiliation":[{"name":"University of Turin","place":["Italy"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michele","family":"Mondini","sequence":"additional","affiliation":[{"name":"University of Eastern Piedmont \u201cA. Avogadro,\u201d Novara","place":["Italy"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudia","family":"Zannetti","sequence":"additional","affiliation":[{"name":"International Agency for Research on Cancer","place":["France"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Benedetta","family":"Bussolati","sequence":"additional","affiliation":[{"name":"Center for Experimental Research and Medical Studies","place":["Italy"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gianni","family":"Camussi","sequence":"additional","affiliation":[{"name":"Center for Experimental Research and Medical Studies","place":["Italy"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marisa","family":"Gariglio","sequence":"additional","affiliation":[{"name":"University of Eastern Piedmont \u201cA. Avogadro,\u201d Novara","place":["Italy"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Santo","family":"Landolfo","sequence":"additional","affiliation":[{"name":"University of Turin","place":["Italy"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2011,8,1]]},"reference":[{"key":"e_1_3_3_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcmd.2003.10.002"},{"key":"e_1_3_3_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200939995"},{"key":"e_1_3_3_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)63540-7"},{"key":"e_1_3_3_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00109-006-0075-z"},{"key":"e_1_3_3_6_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.02-0557fje"},{"key":"e_1_3_3_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.78.6.3190-3195.2004"},{"key":"e_1_3_3_8_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M701846200"},{"key":"e_1_3_3_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/35025220"},{"key":"e_1_3_3_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2006.04.056"},{"key":"e_1_3_3_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2393"},{"key":"e_1_3_3_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2007.06.008"},{"key":"e_1_3_3_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm0195-27"},{"key":"e_1_3_3_14_1","doi-asserted-by":"publisher","DOI":"10.1089\/107999002320271413"},{"key":"e_1_3_3_15_1","doi-asserted-by":"publisher","DOI":"10.1089\/jir.2010.0106"},{"key":"e_1_3_3_16_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0800835105"},{"key":"e_1_3_3_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910580313"},{"key":"e_1_3_3_18_1","first-page":"381","article-title":"Isolation and characterization of human breast tumor-derived endothelial cells","volume":"15","author":"Grange C","year":"2006","unstructured":"Grange C, , Bussolati B, , Bruno S, , Fonsato V, , Sapino A, , Camussi G. 2006. Isolation and characterization of human breast tumor-derived endothelial cells. Oncolog Rep, 15:381\u2013386.","journal-title":"Oncolog Rep"},{"key":"e_1_3_3_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/cdg382"},{"key":"e_1_3_3_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaut.2010.04.001"},{"key":"e_1_3_3_21_1","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.0904507"},{"key":"e_1_3_3_22_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-05-0002"},{"key":"e_1_3_3_23_1","first-page":"2977","article-title":"Drug resistance and P-glycoprotein expression in endothelial cells of newly formed capillaries induced by tumors","volume":"18","author":"Iwahana M","year":"1998","unstructured":"Iwahana M, , Utoguchi N, , Mayumi T, , Goryo M, , Okada K. 1998. Drug resistance and P-glycoprotein expression in endothelial cells of newly formed capillaries induced by tumors. Anticancer Res, 18:2977\u20132980.","journal-title":"Anticancer Res"},{"key":"e_1_3_3_24_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1026510130114"},{"key":"e_1_3_3_25_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-1327.2000.01421.x"},{"key":"e_1_3_3_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9378(95)90579-0"},{"key":"e_1_3_3_27_1","first-page":"45S","article-title":"Clinical trials of angiogenic drugs: opportunities, problems, and assessment of initial results","volume":"19","author":"Kerbel RS","year":"2001","unstructured":"Kerbel RS. 2001. Clinical trials of angiogenic drugs: opportunities, problems, and assessment of initial results. J Clin Oncol, 19:45S\u201351S.","journal-title":"J Clin Oncol"},{"key":"e_1_3_3_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cytogfr.2008.11.002"},{"key":"e_1_3_3_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2005.03.032"},{"key":"e_1_3_3_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)64920-6"},{"key":"e_1_3_3_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2003.10.014"},{"key":"e_1_3_3_32_1","first-page":"33","article-title":"Biological response modifiers in cancer","volume":"8","author":"Reang P","year":"2006","unstructured":"Reang P, , Gupta M, , Kohli K. 2006. Biological response modifiers in cancer. Med Gen Med, 8:33\u201339.","journal-title":"Med Gen Med"},{"key":"e_1_3_3_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri2314"},{"key":"e_1_3_3_34_1","first-page":"233","article-title":"The interferon-stimulated genes: targets of direct signalling by interferons, double-stranded RNA, and viruses","volume":"316","author":"Sen GC","year":"2007","unstructured":"Sen GC, , Sarkar SN. 2007. The interferon-stimulated genes: targets of direct signalling by interferons, double-stranded RNA, and viruses. Curr Top Microbiol Immunol, 316:233\u2013250.","journal-title":"Curr Top Microbiol Immunol"},{"key":"e_1_3_3_35_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.10.4562"},{"key":"e_1_3_3_36_1","doi-asserted-by":"publisher","DOI":"10.1089\/jir.1996.16.577"},{"key":"e_1_3_3_37_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00418-002-0485-0"},{"key":"e_1_3_3_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbapap.2008.04.004"}],"container-title":["Journal of Interferon &amp; Cytokine Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1089\/jir.2011.0001","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/jir.2011.0001","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/jir.2011.0001","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T09:31:56Z","timestamp":1777455116000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1089\/jir.2011.0001"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,8]]},"references-count":37,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2011,8]]}},"alternative-id":["10.1089\/jir.2011.0001"],"URL":"https:\/\/doi.org\/10.1089\/jir.2011.0001","relation":{},"ISSN":["1079-9907","1557-7465"],"issn-type":[{"value":"1079-9907","type":"print"},{"value":"1557-7465","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,8]]}}}