{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T06:16:32Z","timestamp":1777702592485,"version":"3.51.4"},"reference-count":23,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2023,4,1]],"date-time":"2023-04-01T00:00:00Z","timestamp":1680307200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Journal of Interferon &amp; Cytokine Research"],"published-print":{"date-parts":[[2023,4]]},"abstract":"<jats:p>\n                    Acute myeloid leukemia (AML) is characterized by an increased proliferation and loss of differentiation of hematopoietic myeloid progenitors or precursors. Studies performed in AML-affected patients revealed a T cell deficiency characterized by a reduced thymic output and peripheral functional abnormalities. To assess for the thymus function during AML, we used an AML mouse model and showed a drastic thymic atrophy. We observed a massive loss among double (CD4\n                    <jats:sup>+<\/jats:sup>\n                    CD8\n                    <jats:sup>+<\/jats:sup>\n                    - DP) and single positive (CD4\n                    <jats:sup>+<\/jats:sup>\n                    \/8\n                    <jats:sup>+<\/jats:sup>\n                    - SP) thymocytes. We assessed for the expression of different actors of cell death signalling pathways by RT-qPCR or Western blotting. When comparing leukemic to control mice, there was a significant increase in the expression of\n                    <jats:italic toggle=\"yes\">Mlkl<\/jats:italic>\n                    gene, phosphorylated MLKL and RIPK3 proteins, and tumor necrosis factor (TNF)-alpha receptors 1 on DP and SP thymocytes. These findings revealed a necroptosis cell death which was also observed\n                    <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                    when using cultured wild-type thymocytes and recombinant TNF-alpha protein. Thus, we demonstrated that TNF-alpha plays a deleterious role in thymic function during AML by contributing to extensive thymocytes' death.\n                  <\/jats:p>","DOI":"10.1089\/jir.2022.0229","type":"journal-article","created":{"date-parts":[[2023,3,16]],"date-time":"2023-03-16T16:56:21Z","timestamp":1678985781000},"page":"164-172","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":1,"title":["Tumor Necrosis Factor-Alpha\/Tumor Necrosis Factor-Alpha Receptor 1 Signaling Pathway Leads to Thymocytes' Cell Death by Necroptosis in a Mouse Model of Acute Myeloid Leukemia"],"prefix":"10.1177","volume":"43","author":[{"given":"Meriem","family":"Ben Khoud","sequence":"first","affiliation":[{"name":"University of Lille, CNRS, Inserm, CHU Lille, UMR9020-U1277 \u2013 CANTHER - Cancer Heterogeneity Plasticity and Resistance to Therapies, Lille, France."},{"name":"Institut Pour la Recherche sur le Cancer de Lille (IRCL), Lille, France."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nathalie","family":"Jouy","sequence":"additional","affiliation":[{"name":"University of Lille, UAR2014 - US 41 - Plateformes Lilloises en Biologie &amp; Sant\u00e9- BioImaging Center Lille (BICeL) - Plateau de Cytom\u00e9trie en Flux, Lille, France."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Virginie","family":"Driss","sequence":"additional","affiliation":[{"name":"University of Lille, CNRS, Inserm, CHU Lille, UMR9020-U1277 \u2013 CANTHER - Cancer Heterogeneity Plasticity and Resistance to Therapies, Lille, France."},{"name":"Institut Pour la Recherche sur le Cancer de Lille (IRCL), Lille, France."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bruno","family":"Quesnel","sequence":"additional","affiliation":[{"name":"University of Lille, CNRS, Inserm, CHU Lille, UMR9020-U1277 \u2013 CANTHER - Cancer Heterogeneity Plasticity and Resistance to Therapies, Lille, France."},{"name":"Institut Pour la Recherche sur le Cancer de Lille (IRCL), Lille, France."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4104-7732","authenticated-orcid":false,"given":"Carine","family":"Brinster","sequence":"additional","affiliation":[{"name":"University of Lille, CNRS, Inserm, CHU Lille, UMR9020-U1277 \u2013 CANTHER - Cancer Heterogeneity Plasticity and Resistance to Therapies, Lille, France."},{"name":"Institut Pour la Recherche sur le Cancer de Lille (IRCL), Lille, France."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2023,4,12]]},"reference":[{"key":"e_1_3_6_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-26282-z"},{"key":"e_1_3_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2016.07.016"},{"key":"e_1_3_6_4_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.165.10.5621"},{"key":"e_1_3_6_5_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20110251"},{"key":"e_1_3_6_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/imr.12532"},{"key":"e_1_3_6_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.201444736"},{"key":"e_1_3_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.imbio.2011.03.009"},{"key":"e_1_3_6_9_1","doi-asserted-by":"crossref","unstructured":"Giroir BP Brow T Beutler B. 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Serum tumor necrosis factor-alpha levels in acute leukemia and its prognostic significance. 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