{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T05:47:41Z","timestamp":1777700861972,"version":"3.51.4"},"reference-count":22,"publisher":"SAGE Publications","issue":"12","license":[{"start":{"date-parts":[[2024,12,1]],"date-time":"2024-12-01T00:00:00Z","timestamp":1733011200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Journal of Interferon &amp; Cytokine Research"],"published-print":{"date-parts":[[2024,12]]},"abstract":"<jats:p>\n                    Toll-like receptors (TLRs), particularly the TLR2, take part in the elicitation of immune responses against\n                    <jats:italic toggle=\"yes\">Entamoeba histolytica<\/jats:italic>\n                    . This study aimed to investigate the relationship between a specific polymorphism called rs3804099 in the\n                    <jats:italic toggle=\"yes\">TLR2<\/jats:italic>\n                    gene and\n                    <jats:italic toggle=\"yes\">E. histolytica\/E. dispar\/E. moshkovskii<\/jats:italic>\n                    infection among Lebanese children. A case-control study encompassed 180 participants including 68 children with amebiasis and 112 matched controls. Blood samples were collected, and genomic DNA was extracted using the classical proteinase K digestion and phenol\u2013chloroform extraction method. The variant rs3804099 was examined using the Amplification Refractory Mutation System Polymerase Chain Reaction. The accuracy of the genotyping was supported by sequencing 5% of samples. The\n                    <jats:italic toggle=\"yes\">TLR2<\/jats:italic>\n                    rs3804099 polymorphism was identified in the studied population, and the observed genotypic distributions were consistent with\n                    <jats:italic toggle=\"yes\">Hardy\u2013Weinberg<\/jats:italic>\n                    equilibrium (\n                    <jats:italic toggle=\"yes\">P<\/jats:italic>\n                    &gt; 0.05). The frequency of the rare CC genotype was significantly higher in patients compared to the noninfected group (\n                    <jats:italic toggle=\"yes\">P<\/jats:italic>\n                    &lt; 0.01). In controls, the homozygous TT genotype was less frequent than the heterozygous CT genotype. The rare CC genotype was associated with a higher risk of amebiasis among children (odds ratios = 3.27,\n                    <jats:italic toggle=\"yes\">P<\/jats:italic>\n                    = 0.002). These findings provide evidence supporting the association between the rs3804099 SNP in the\n                    <jats:italic toggle=\"yes\">TLR2<\/jats:italic>\n                    gene and\n                    <jats:italic toggle=\"yes\">E. histolytica\/E. dispar\/E. moshkovskii<\/jats:italic>\n                    infection among Lebanese children.\n                  <\/jats:p>","DOI":"10.1089\/jir.2024.0183","type":"journal-article","created":{"date-parts":[[2024,10,21]],"date-time":"2024-10-21T05:33:22Z","timestamp":1729488802000},"page":"550-556","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Association of the Single Nucleotide Polymorphism 19216T\/C in the\n                    <i>TLR2<\/i>\n                    Gene (rs3804099) with\n                    <i>Entamoeba histolytica\/Entamoeba dispar\/Entamoeba moshkovskii<\/i>\n                    Infection Among Lebanese Children"],"prefix":"10.1177","volume":"44","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-7443-4074","authenticated-orcid":false,"given":"Israa","family":"Dib","sequence":"first","affiliation":[{"name":"Laboratory of Water Environment and Urban Systems, University Paris-Est Cr\u00e9teil, Cr\u00e9teil Cedex, France."},{"name":"Environmental Health Research Laboratory, Faculty of Sciences V, Lebanese University, Nabatieh, Lebanon."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiba","family":"Noureddine","sequence":"additional","affiliation":[{"name":"Environmental Health Research Laboratory, Faculty of Sciences V, Lebanese University, Nabatieh, Lebanon."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohamad","family":"Fakih","sequence":"additional","affiliation":[{"name":"Environmental Health Research Laboratory, Faculty of Sciences V, Lebanese University, Nabatieh, Lebanon."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexandre","family":"Livet","sequence":"additional","affiliation":[{"name":"Laboratory of Water Environment and Urban Systems, University Paris-Est Cr\u00e9teil, Cr\u00e9teil Cedex, France."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vanessa","family":"Alphonse","sequence":"additional","affiliation":[{"name":"Laboratory of Water Environment and Urban Systems, University Paris-Est Cr\u00e9teil, Cr\u00e9teil Cedex, France."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abbas","family":"Illayk","sequence":"additional","affiliation":[{"name":"Math Department, Faculty of Sciences V, Lebanese University, Nabatieh, Lebanon."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abdallah Ahmad","family":"Medlej","sequence":"additional","affiliation":[{"name":"Molecular Genetics Department, Sheikh Ragheb Harb University Hospital, Nabatieh, Lebanon."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mahdi","family":"Tarhini","sequence":"additional","affiliation":[{"name":"Faculty of Health Sciences, Al Maaref University, and Director of Laboratories and Blood Bank Department, Sheikh Ragheb Harb University Hospital, Nabatieh, Lebanon."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Noureddine","family":"Bousserrhine","sequence":"additional","affiliation":[{"name":"Laboratory of Water Environment and Urban Systems, University Paris-Est Cr\u00e9teil, Cr\u00e9teil Cedex, France."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2024,12,6]]},"reference":[{"key":"e_1_3_7_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01335-06"},{"key":"e_1_3_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molbiopara.2005.10.013"},{"key":"e_1_3_7_4_1","doi-asserted-by":"publisher","DOI":"10.1086\/381272"},{"key":"e_1_3_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.parint.2009.10.010"},{"key":"e_1_3_7_6_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.13-12878"},{"key":"e_1_3_7_7_1","doi-asserted-by":"publisher","DOI":"10.5223\/pghn.2018.21.3.176"},{"key":"e_1_3_7_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/11.4.45r"},{"key":"e_1_3_7_9_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra022710"},{"key":"e_1_3_7_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/hel.12292"},{"key":"e_1_3_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2014.01.009"},{"key":"e_1_3_7_12_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.03-0578fje"},{"key":"e_1_3_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2016.06.028"},{"key":"e_1_3_7_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3024.2005.00754.x"},{"key":"e_1_3_7_15_1","doi-asserted-by":"publisher","DOI":"10.5152\/tjg.2018.17484"},{"key":"e_1_3_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bjid.2012.12.009"},{"key":"e_1_3_7_17_1","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/192420"},{"key":"e_1_3_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0169-4758(00)01730-0"},{"key":"e_1_3_7_19_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v20.i18.5244"},{"key":"e_1_3_7_20_1","doi-asserted-by":"publisher","DOI":"10.1152\/physiol.00038.2019"},{"issue":"1","key":"e_1_3_7_21_1","first-page":"13","article-title":"A 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