{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T23:03:56Z","timestamp":1781737436197,"version":"3.54.5"},"reference-count":31,"publisher":"SAGE Publications","issue":"12","license":[{"start":{"date-parts":[[2012,12,1]],"date-time":"2012-12-01T00:00:00Z","timestamp":1354320000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medicinal Food"],"published-print":{"date-parts":[[2012,12]]},"abstract":"<jats:p>\n                    The triterpene chikusetsusaponin IVa was isolated from the fruit of\n                    <jats:italic toggle=\"yes\">Ilex paraguariensis<\/jats:italic>\n                    . Using biochemical and pharmacological methods, we demonstrated that chikusetsusaponin IVa (1) prolongs the recalcification time, prothrombin time, activated partial thromboplastin time, and thrombin time of normal human plasma in a dose-dependent manner, (2) inhibits the amidolytic activity of thrombin and factor Xa upon synthetic substrates S2238 and S2222, (3) inhibits thrombin-induced fibrinogen clotting (50% inhibition concentration, 199.4\u00b19.1\u2009\u03bc\n                    <jats:italic toggle=\"yes\">M<\/jats:italic>\n                    ), and (4) inhibits thrombin- and collagen-induced platelet aggregation. The results also indicate that chikusetsusaponin IVa preferentially inhibits thrombin in a competitive manner (\n                    <jats:italic toggle=\"yes\">\n                      K\n                      <jats:sub>i<\/jats:sub>\n                    <\/jats:italic>\n                    =219.6\u2009\u03bc\n                    <jats:italic toggle=\"yes\">M<\/jats:italic>\n                    ). Furthermore, when administered intravenously to rats, chikusetsusaponin IVa inhibited thrombus formation in a stasis model of venous thrombosis, although it did not induce a significant bleeding effect. Chikusetsusaponin IVa also prolonged the\n                    <jats:italic toggle=\"yes\">ex vivo<\/jats:italic>\n                    activated partial thromboplastin time. Altogether, these data suggest that chikusetsusaponin IVa exerts antithrombotic effects, including minor hemorrhagic events. This appears to be important for the development of new therapeutic agents.\n                  <\/jats:p>","DOI":"10.1089\/jmf.2011.0320","type":"journal-article","created":{"date-parts":[[2012,11,7]],"date-time":"2012-11-07T09:20:11Z","timestamp":1352280011000},"page":"1073-1080","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":38,"title":["Antithrombotic Effect of Chikusetsusaponin IVa Isolated from\n                    <i>Ilex paraguariensis<\/i>\n                    (Mat\u00e9)"],"prefix":"10.1177","volume":"15","author":[{"given":"Tabitha","family":"Dahmer","sequence":"first","affiliation":[{"name":"Federal University of Rio Grande do Sul","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Markus","family":"Berger","sequence":"additional","affiliation":[{"name":"Federal University of Rio Grande do Sul","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adriana Gregory","family":"Barlette","sequence":"additional","affiliation":[{"name":"Federal University of Rio Grande do Sul","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Jr.","given":"Jos\u00e9","family":"Reck","sequence":"additional","affiliation":[{"name":"Federal University of Rio Grande do Sul","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00e9ferson","family":"Segalin","sequence":"additional","affiliation":[{"name":"Federal University of Rio Grande do Sul","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simone","family":"Verza","sequence":"additional","affiliation":[{"name":"Federal University of Rio Grande do Sul","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"George 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