{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T15:36:17Z","timestamp":1777908977528,"version":"3.51.4"},"reference-count":28,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2012,4,1]],"date-time":"2012-04-01T00:00:00Z","timestamp":1333238400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medicinal Food"],"published-print":{"date-parts":[[2012,4]]},"abstract":"\n The prophylactic effects of\n Hijikia fusiforme<\/jats:italic>\n on bone metabolism were examined using\n in vitro<\/jats:italic>\n indices of bone formation and resorption. As the indices of bone formation, osteoblast proliferation and differentiation were measured through mitochondrial enzyme activity [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay] and bone marker alkaline phosphatase (ALP) activity. The aqueous extract of\n H. fusiforme<\/jats:italic>\n stimulated the proliferation of the human osteoblast-like cell line MG63 and the ALP activity of the mouse osteoblast-like cell line MC3T3-E1. Moreover, extracellular matrix mineralization was accelerated by the addition of\n H. fusiforme.<\/jats:italic>\n As the indices of bone resorption, differentiation of the murine macrophage\/osteoclast precursor cell line RAW 264.7 by receptor activator of nuclear factor-\u03baB ligand (RANKL) was measured as tartrate-resistant acid phosphatase\u2013positive multinucleated cells, which were suppressed by\n H. fusiforme<\/jats:italic>\n . Additionally,\n H. fusiforme<\/jats:italic>\n lowered the secreted amount of RANKL that is required for the osteoclastic differentiation and activation, but the amount of osteoprotegerin as a decoy receptor for RANKL was not affected. The bone-protective effects of\n H. fusiforme<\/jats:italic>\n in estrogen-deficient ovariectomized rats were also investigated. Osteoporosis was induced in female Sprague\u2013Dawley rats by ovariectomy for 15 weeks, and then\n H. fusiforme<\/jats:italic>\n was orally administered at a dose of 100\u2009mg\/kg of body weight every day for 6 weeks. Bone mineral density in the group orally administered\n H. fusiforme<\/jats:italic>\n was increased, compared with ovariectomized rats, but not significantly (\n P<\/jats:italic>\n >.05). Oral administration of\n H. fusiforme<\/jats:italic>\n improved microarchitecture of bone in terms of bone volume (bone volume\/total volume ratio) and trabecular separation (\n P<\/jats:italic>\n <.05). The amounts of osteocalcin and C-telopeptide type I collagen in serum were measured as the biomarkers for bone formation and resorption. The level of osteocalcin in serum was increased, but not significantly. However, the level of C-telopeptide type I collagen in serum was significantly decreased (\n P<\/jats:italic>\n <.05). When the results are taken together, the present study indicates that\n H. fusiforme<\/jats:italic>\n might be useful in the treatment of osteoporosis.\n <\/jats:p>","DOI":"10.1089\/jmf.2011.1827","type":"journal-article","created":{"date-parts":[[2012,2,8]],"date-time":"2012-02-08T12:18:11Z","timestamp":1328703491000},"page":"384-390","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":2,"title":["Hijikia fusiforme<\/i>\n Protects Against Ovariectomy-Induced Bone Loss in Rats"],"prefix":"10.1177","volume":"15","author":[{"given":"Sang-Yong","family":"Park","sequence":"first","affiliation":[{"name":"Kyung Hee University"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jung Min","family":"Lee","sequence":"additional","affiliation":[{"name":"Kyung Hee University"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Don-Gil","family":"Lee","sequence":"additional","affiliation":[{"name":"Kyung Hee University"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heon-Sub","family":"Shin","sequence":"additional","affiliation":[{"name":"Kyung Hee University"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jung Eun","family":"Yang","sequence":"additional","affiliation":[{"name":"Kyung Hee University"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eun Ji","family":"Yi","sequence":"additional","affiliation":[{"name":"Kyung Hee University"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jae Hee","family":"Park","sequence":"additional","affiliation":[{"name":"Kyung Hee University"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu Jin","family":"Jeon","sequence":"additional","affiliation":[{"name":"Jeju National University"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Da-Eun","family":"Nam","sequence":"additional","affiliation":[{"name":"Kyung Hee University"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tae-Hoo","family":"Yi","sequence":"additional","affiliation":[{"name":"Kyung Hee University"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2012,4,1]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01719785"},{"key":"e_1_3_3_3_2","first-page":"5","article-title":"Pathogenesis of postmenopausal osteoporosis","volume":"2","author":"Lawrence G","year":"2002","unstructured":"Lawrence G, , Raisz MD. Pathogenesis of postmenopausal osteoporosis. Rev Endocr Metab Disord, 2002; 2:5\u201312.","journal-title":"Rev Endocr Metab Disord"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2010.05.016"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1590\/S0004-27302010000200007"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10238-010-0117-2"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1007\/s001980170079"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00198-008-0660-9"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199506153322401"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tem.2005.05.001"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.31819"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1080\/01635589709514505"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.4142\/jvs.2008.9.4.359"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)43072-4"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)52451-6"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature01658"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1210\/endo.142.3.8031"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/0031-9384(87)90235-6"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cytogfr.2003.10.005"},{"key":"e_1_3_3_20_2","first-page":"424","article-title":"The laboratory rat as an animal model for osteoporosis research","volume":"58","author":"Lelovas PP","year":"2008","unstructured":"Lelovas PP, , Xanthos TT, , Thoma SE et al. The laboratory rat as an animal model for osteoporosis research. Comp Med, 2008; 58:424\u2013430.","journal-title":"Comp Med"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0703610104"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2004-09-3662"},{"key":"e_1_3_3_23_2","first-page":"435","article-title":"Antioxidant activity on ethanol extract from boiled-water of Hizikia fusiformis","volume":"48","author":"Park KE","year":"2005","unstructured":"Park KE, , Jang MS, , Lim CW et al. Antioxidant activity on ethanol extract from boiled-water of Hizikia fusiformis. J Korean Soc Food Sci Nutr, 2005; 48:435\u2013439.","journal-title":"J Korean Soc Food Sci Nutr"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.3746\/jkfn.2002.31.1.087"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1186\/1742-4933-2-14"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11914-009-0023-2"},{"key":"e_1_3_3_27_2","first-page":"113","article-title":"Osteoporosis: integrating biomarkers and other diagnostic correlates into the management of bone fragility","volume":"12","author":"McCormick RK","year":"2007","unstructured":"McCormick RK. Osteoporosis: integrating biomarkers and other diagnostic correlates into the management of bone fragility. Altern Med Rev, 2007; 12:113\u2013145.","journal-title":"Altern Med Rev"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11154-010-9154-0"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1021\/jf0721500"}],"container-title":["Journal of Medicinal Food"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1089\/jmf.2011.1827","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/jmf.2011.1827","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/jmf.2011.1827","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T14:19:32Z","timestamp":1777645172000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1089\/jmf.2011.1827"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,4]]},"references-count":28,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2012,4]]}},"alternative-id":["10.1089\/jmf.2011.1827"],"URL":"https:\/\/doi.org\/10.1089\/jmf.2011.1827","relation":{},"ISSN":["1096-620X","1557-7600"],"issn-type":[{"value":"1096-620X","type":"print"},{"value":"1557-7600","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,4]]}}}