{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T15:37:21Z","timestamp":1777909041801,"version":"3.51.4"},"reference-count":41,"publisher":"SAGE Publications","issue":"6","license":[{"start":{"date-parts":[[2013,6,1]],"date-time":"2013-06-01T00:00:00Z","timestamp":1370044800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medicinal Food"],"published-print":{"date-parts":[[2013,6]]},"abstract":"<jats:p>\n                    Liver fibrosis is the result of chronic liver injury, and it represents a widespread medical problem. The aim of this study is to investigate the antifibrotic activity of isoquinoline alkaloid berberine in carbon tetrachloride (CCl\n                    <jats:sub>4<\/jats:sub>\n                    )-induced damage in mice. Hepatic fibrosis was induced by intraperitoneal (i.p.) administration of CCl\n                    <jats:sub>4<\/jats:sub>\n                    (2\u2009mL\/kg, 20% v\/v in olive oil) twice a week for 8 weeks. Berberine at the doses of 3 and 9\u2009mg\/kg and silymarin at the dose of 50\u2009mg\/kg were given i.p. once daily for the next 2 weeks. CCl\n                    <jats:sub>4<\/jats:sub>\n                    intoxication increased the levels of serum transaminases and induced oxidative stress in the liver. Hepatic fibrosis was evidenced by a massive deposition of collagen, which coincided with increased expression of tumor necrosis factor (TNF)\u2013\n                    <jats:italic toggle=\"yes\">\u03b1<\/jats:italic>\n                    and transforming growth factor (TGF)\u2013\n                    <jats:italic toggle=\"yes\">\u03b2<\/jats:italic>\n                    1 and the activation of hepatic stellate cells. The high-dose berberine (9\u2009mg\/kg) ameliorated oxidative stress, decreased TNF-\n                    <jats:italic toggle=\"yes\">\u03b1<\/jats:italic>\n                    and TGF-\n                    <jats:italic toggle=\"yes\">\u03b2<\/jats:italic>\n                    1 expression, increased the levels of matrix metalloproteinase (MMP)\u20132, and stimulated the elimination of fibrous deposits. Berberine at the dose of 9\u2009mg\/kg exhibited stronger therapeutic activity against hepatic fibrosis than silymarin at the dose of 50\u2009mg\/kg.\n                    <jats:italic toggle=\"yes\">In vitro<\/jats:italic>\n                    analyses show an important scavenging activity of berberine against oxygen and nitrogen reactive species. The results of this study suggest that berberine could ameliorate liver fibrosis through the suppression of hepatic oxidative stress and fibrogenic potential, concomitantly stimulating the degradation of collagen deposits by MMP-2.\n                  <\/jats:p>","DOI":"10.1089\/jmf.2012.0175","type":"journal-article","created":{"date-parts":[[2013,6,4]],"date-time":"2013-06-04T09:56:28Z","timestamp":1370339788000},"page":"518-528","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":40,"title":["Resolution of Liver Fibrosis by Isoquinoline Alkaloid Berberine in CCl\n                    <sub>4<\/sub>\n                    -Intoxicated Mice Is Mediated by Suppression of Oxidative Stress and Upregulation of MMP-2 Expression"],"prefix":"10.1177","volume":"16","author":[{"given":"Robert","family":"Domitrovi\u0107","sequence":"first","affiliation":[{"name":"University of Rijeka","place":["Croatia"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hrvoje","family":"Jakovac","sequence":"additional","affiliation":[{"name":"University of Rijeka","place":["Croatia"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vanja Vasiljev","family":"Marchesi","sequence":"additional","affiliation":[{"name":"University of Rijeka","place":["Croatia"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Biljana","family":"Bla\u017eekovi\u0107","sequence":"additional","affiliation":[{"name":"University of Zagreb","place":["Croatia"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2013,6,1]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-8278(01)00142-8"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.275.4.2247"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/s004180000150"},{"key":"e_1_3_3_5_2","first-page":"94","article-title":"Berberine: therapeutic potential of an alkaloid found in several medicinal plants","volume":"2","author":"Birdsall TC","year":"1997","unstructured":"Birdsall TC, , Kelly GS. 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