{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,22]],"date-time":"2026-05-22T07:08:08Z","timestamp":1779433688390,"version":"3.53.1"},"reference-count":31,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2017,3,1]],"date-time":"2017-03-01T00:00:00Z","timestamp":1488326400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medicinal Food"],"published-print":{"date-parts":[[2017,3]]},"abstract":"<jats:p>\n                    The transport behavior and absorption mechanism of Ala-His-Leu-Leu (AHLL) intestinal absorption in Caco-2 cell monolayers were clarified systemically. The safe absorptive concentration of AHLL was 200\u2009\n                    <jats:italic toggle=\"yes\">\u03bc<\/jats:italic>\n                    g\/mL, which was determined by the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide assay. The permeation of AHLL was concentration dependent in a bidirectional transfer and reached a plateau at 90\u2009min. The efflux ratio was above 0.5, suggesting that AHLL was absorbed by both active transport and passive diffusion. The apparent permeability coefficients (P\n                    <jats:sub>app<\/jats:sub>\n                    ) of AHLL both from the apical (AP) to basolateral (BL) side (P\n                    <jats:sub>app<\/jats:sub>\n                    AB) and from the BL to AP side (P\n                    <jats:sub>app<\/jats:sub>\n                    BA) decreased when the temperature was lowered from 37\u00b0C to 4\u00b0C.The uptake of AHLL was more at pH 7.4 than at other pHs. Both verapamil and (E)-3-[[[3-[2-(7-chloro-2- quinolinyl) ethenyl] phenyl]-[[(3-dimethyl amino)-3-oxopropyl]thio] methyl] thio]-propanoic acid (MK571) inhibited the absorption of AHLL, indicating that P-glycoprotein and multi-drug resistant proteins (MRPs) were all involved in AHLL secretion, especially multi-drug resistant protein 2 (MRP2). AHLL was transported through both trans- and paracellular pathways across the Caco-2 cell monolayer. This work first elucidates the AHLL absorption mechanism in Caco-2 cells and provides the basis for future studies on the improvement of bioavailability.\n                  <\/jats:p>","DOI":"10.1089\/jmf.2016.3842","type":"journal-article","created":{"date-parts":[[2017,2,21]],"date-time":"2017-02-21T14:57:35Z","timestamp":1487689055000},"page":"243-250","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":15,"title":["Transport of a Novel Angiotensin-I-Converting Enzyme Inhibitory Peptide Ala-His-Leu-Leu Across Human Intestinal Epithelial Caco-2 Cells"],"prefix":"10.1177","volume":"20","author":[{"given":"Ying","family":"Li","sequence":"first","affiliation":[{"name":"Institute of Agricultural Products Processing","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiangtao","family":"Zhao","sequence":"additional","affiliation":[{"name":"Institute of Agricultural Products Processing","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoli","family":"Liu","sequence":"additional","affiliation":[{"name":"Institute of Agricultural Products Processing","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiudong","family":"Xia","sequence":"additional","affiliation":[{"name":"Institute of Agricultural Products Processing","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of Agricultural Products Processing","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianzhong","family":"Zhou","sequence":"additional","affiliation":[{"name":"Institute of Agricultural Products Processing","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2017,3,1]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1357-2725(02)00262-5"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1089\/jmf.2014.0028"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.97.14.1411"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2010.06.099"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0196-9781(03)00174-8"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.procbio.2004.08.010"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1021\/jf204118n"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1089\/jmf.2015.3483"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.peptides.2004.01.015"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tiv.2013.12.002"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2012.10.137"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1995-7645(14)60155-3"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tiv.2012.03.009"},{"key":"e_1_3_3_15_2","first-page":"502","article-title":"Assessment of iron bioavailability in ten kinds of Chinese wheat flours using an in vitro digestion\/Caco-2 cell model","volume":"25","author":"Lei J","year":"2012","unstructured":"Lei J, , Zhang Y, , Chen XG, , Zhang MQ, , Bai L, , Huang CY, , Ivan OM: Assessment of iron bioavailability in ten kinds of Chinese wheat flours using an in vitro digestion\/Caco-2 cell model. Biomed Environ Sci, 2012; 25:502\u2013508.","journal-title":"Biomed Environ Sci"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pestbp.2014.04.002"},{"key":"e_1_3_3_17_2","first-page":"354","article-title":"Absorption mechanism of the antihypertensive peptide Val-Leu-Pro-Val-Pro in Caco-2 cell model","volume":"30","author":"Liu D","year":"2008","unstructured":"Liu D, , Sun HY, , Lei L, , Li SM: Absorption mechanism of the antihypertensive peptide Val-Leu-Pro-Val-Pro in Caco-2 cell model. Acta Nutr Sin, 2008; 30:354\u2013362.","journal-title":"Acta Nutr Sin"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1021\/jf072277p"},{"key":"e_1_3_3_19_2","first-page":"21","volume-title":"Counting Cells, Cell Biology A Laboratory Handbook","author":"Celis JE","year":"2008","unstructured":"Celis JE: Counting Cells, Cell Biology A Laboratory Handbook, Vol. 1. Elsevier Academic Press, Beijing, China, 2008, pp. 21\u201324."},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.3109\/10717541003706273"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2012.12.037"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2013.12.069"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tiv.2014.09.013"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2007.303"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2012.12.037"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2014.02.003"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2008.06.090"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1025001131513"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.taap.2004.10.012"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.104.068775"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(03)00059-0"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejps.2010.10.001"}],"container-title":["Journal of Medicinal Food"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1089\/jmf.2016.3842","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/jmf.2016.3842","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/jmf.2016.3842","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,22]],"date-time":"2026-05-22T06:42:05Z","timestamp":1779432125000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1089\/jmf.2016.3842"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,3]]},"references-count":31,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2017,3]]}},"alternative-id":["10.1089\/jmf.2016.3842"],"URL":"https:\/\/doi.org\/10.1089\/jmf.2016.3842","relation":{},"ISSN":["1096-620X","1557-7600"],"issn-type":[{"value":"1096-620X","type":"print"},{"value":"1557-7600","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,3]]}}}