{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T10:58:03Z","timestamp":1780397883577,"version":"3.54.1"},"reference-count":44,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2017,3,1]],"date-time":"2017-03-01T00:00:00Z","timestamp":1488326400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medicinal Food"],"published-print":{"date-parts":[[2017,3]]},"abstract":"<jats:p>\n                    <jats:italic toggle=\"yes\">Gelidium amansii<\/jats:italic>\n                    is an edible and economically important red alga consumed in South Eastern Asia. In previous studies, we reported that the ethanol extracts of\n                    <jats:italic toggle=\"yes\">G. amansii<\/jats:italic>\n                    (GAE) has promising modulatory activity with respect to the morphological and functional maturation of hippocampal neurons in culture. In this study, we show that the chloroform (CHCl\n                    <jats:sub>3<\/jats:sub>\n                    ) subfraction of GAE and the ethyl acetate (EtOAc) fraction dose-dependently promoted neurite outgrowth, and their effects were comparable with that of GAE. We further assessed in cultured cortical neurons, proteins differentially expressed in the presence\/absence of the GAE, CHCl\n                    <jats:sub>3<\/jats:sub>\n                    subfraction, and the EtOAc fraction by 2D-PAGE and matrix-assisted laser desorption\/ionization time-of-flight mass spectrometry. Proteomic data revealed that a number of proteins responsible for multiple cellular and biochemical functions vital for neuronal development and maturation were significantly upregulated in neurons treated with the GAE, CHCl\n                    <jats:sub>3<\/jats:sub>\n                    subfraction, and the EtOAc fraction. Of the identified proteins, profilin 2a, septin 7, cdc42, protein phosphatase 2A, DA11, eukaryotic translation initiation factor 5A-1, and \u03b3-enolase are known to play important roles in neuritogenesis and dendritic arborization. Immunofluorescence data demonstrate that GAE-treated hippocampal neurons showed greater intensity ratios in the expressions of the septin 7 and cdc42 compared to vehicle control, validating their proteomic profiles. Together these results suggest that the GAE\/CHCl\n                    <jats:sub>3<\/jats:sub>\n                    subfraction and EtOAc fraction promote neurite development by up or downregulating several key proteins.\n                  <\/jats:p>","DOI":"10.1089\/jmf.2016.3848","type":"journal-article","created":{"date-parts":[[2017,3,3]],"date-time":"2017-03-03T10:39:21Z","timestamp":1488537561000},"page":"279-287","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":11,"title":["Proteomic Analysis of the Neurotrophic Effect of\n                    <i>Gelidium amansii<\/i>\n                    in Primary Cultured Neurons"],"prefix":"10.1177","volume":"20","author":[{"given":"Md. 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