{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T18:48:12Z","timestamp":1780944492907,"version":"3.54.1"},"reference-count":44,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2019,3,1]],"date-time":"2019-03-01T00:00:00Z","timestamp":1551398400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medicinal Food"],"published-print":{"date-parts":[[2019,3]]},"abstract":"<jats:p>\n                    Mojarra of Nile tilapia (\n                    <jats:italic toggle=\"yes\">Oreochromis niloticus<\/jats:italic>\n                    ) skeleton was used as protein source for the preparation of protein hydrolysates and peptide fractions with angiotensin-converting enzyme (ACE) inhibitory activity. The flour presented a content of 34.92% protein and a brightness (luminosity,\n                    <jats:italic toggle=\"yes\">L*<\/jats:italic>\n                    ) of 82.29. Protein hydrolysates were obtained from the protein-rich flour with the enzymes Flavourzyme\n                    <jats:sup>\u00ae<\/jats:sup>\n                    and Alcalase\n                    <jats:sup>\u00ae<\/jats:sup>\n                    reaching degree of hydrolysis (%DH) of 52% and 67% at 100\u2009min of reaction, respectively. Both hydrolysates showed low-molecular-weight (MW) peptides estimated by sodium dodecyl sulfate\u2013polyacrylamide gel electrophoresis. The hydrolysates obtained with Flavourzyme at 60\u2009min and at 80\u2009min with Alcalase showed greater ACE inhibitory activity with\n                    <jats:italic toggle=\"yes\">\n                      IC\n                      <jats:sub>50<\/jats:sub>\n                    <\/jats:italic>\n                    values of 0.238 and 0.344\u2009mg\/mL, respectively. The peptide fraction A (MW &gt;10\u2009kDa) with Flavourzyme and fraction B (MW\u2009=\u200910\u20135\u2009kDa) with Alcalase obtained by ultrafiltration of hydrolysates with higher DH presented\n                    <jats:italic toggle=\"yes\">\n                      IC\n                      <jats:sub>50<\/jats:sub>\n                    <\/jats:italic>\n                    of 0.728 and 0.354\u2009mg\/mL, respectively, whereas peptide fraction C (MW\u2009=\u20095\u20133\u2009kDa) with both enzymes hydrolysates with greater ACE inhibitory activity showed\n                    <jats:italic toggle=\"yes\">\n                      IC\n                      <jats:sub>50<\/jats:sub>\n                    <\/jats:italic>\n                    values of 0.470 and 0.634\u2009mg\/mL. The components obtained in this study could be used as functional ingredients in the design and development of functional foods.\n                  <\/jats:p>","DOI":"10.1089\/jmf.2018.0163","type":"journal-article","created":{"date-parts":[[2019,3,5]],"date-time":"2019-03-05T11:56:09Z","timestamp":1551786969000},"page":"286-293","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":18,"title":["Angiotensin-Converting Enzyme Inhibition\n                    <i>In Vitro<\/i>\n                    by Protein Hydrolysates and Peptide Fractions from Mojarra of Nile Tilapia (\n                    <i>Oreochromis niloticus<\/i>\n                    ) Skeleton"],"prefix":"10.1177","volume":"22","author":[{"given":"Berenise","family":"Borges-Contreras","sequence":"first","affiliation":[{"name":"National Technology of Mexico\/Technological Institute of Tuxtepec, Tuxtepec, Mexico."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cecilia Eugenia","family":"Mart\u00ednez-S\u00e1nchez","sequence":"additional","affiliation":[{"name":"National Technology of Mexico\/Technological Institute of Tuxtepec, Tuxtepec, Mexico."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erasmo","family":"Herman-Lara","sequence":"additional","affiliation":[{"name":"National Technology of Mexico\/Technological Institute of Tuxtepec, Tuxtepec, Mexico."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jes\u00fas","family":"Rodr\u00edguez-Miranda","sequence":"additional","affiliation":[{"name":"National Technology of Mexico\/Technological Institute of Tuxtepec, Tuxtepec, Mexico."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Betsab\u00e9","family":"Hern\u00e1ndez-Santos","sequence":"additional","affiliation":[{"name":"National Technology of Mexico\/Technological Institute of Tuxtepec, Tuxtepec, Mexico."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Manuel","family":"Ju\u00e1rez-Barrientos","sequence":"additional","affiliation":[{"name":"National Technology of Mexico\/Higher Technological Institute of Tierra Blanca, Tierra Blanca, Mexico."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos Mart\u00edn","family":"Guerra-Almonacid","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Research Group in Natural Products, Faculty of Sciences, University of Tolima, Ibague, Colombia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David Abram","family":"Betancur-Ancona","sequence":"additional","affiliation":[{"name":"Department of Food Science, Faculty of Chemical Engineering, Autonomous University of Yucatan, Merida, Mexico."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan Gabriel","family":"Torruco-Uco","sequence":"additional","affiliation":[{"name":"National Technology of Mexico\/Technological Institute of Tuxtepec, Tuxtepec, Mexico."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2019,3,15]]},"reference":[{"key":"e_1_3_3_2_2","first-page":"313","article-title":"Mice lacking endothelial ACE normal blood pressure with elevated Angiotensin II","volume":"41","author":"Cole JM","year":"2008","unstructured":"Cole JM, , Khokhlova N, , Sutliff RL, , Adams JW, , Disher KM, , Zhao H, , Capecchi MR, , Corvol P, , Bernstein KE: Mice lacking endothelial ACE normal blood pressure with elevated Angiotensin II. 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