{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T10:09:25Z","timestamp":1784542165020,"version":"3.55.0"},"reference-count":17,"publisher":"SAGE Publications","issue":"6","license":[{"start":{"date-parts":[[2017,3,27]],"date-time":"2017-03-27T00:00:00Z","timestamp":1490572800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Journal of Ocular Pharmacology and Therapeutics"],"published-print":{"date-parts":[[2017,7,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Purpose:<\/jats:title>\n                    <jats:p>To investigate the short-term effects of 2 new secretagogue eye drops for dry eye, 3% diquafosol tetrasodium ophthalmic solution (diquafosol) and 2% rebamipide ophthalmic suspension (rebamipide), on the concentration of mucin 5AC (MUC5AC) in rabbit tear fluid and conjunctival goblet cells.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>\n                      One dose of artificial tears, diquafosol or rebamipide, was instilled into 8 eyes of Japanese white rabbits. MUC5AC concentration in the tear fluid was examined using the enzyme-linked immunosorbent assay 15\u2009min after instillation and compared with 8 untreated controls. Impression cytology was performed to measure the number of periodic acid Schiff (PAS)-positive cells and the ratio of the PAS-positive area using image analysis software. Statistical comparison was performed using ANOVA with\n                      <jats:italic toggle=\"yes\">post hoc<\/jats:italic>\n                      analysis with the Tukey's test.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      After 15\u2009min, only diquafosol significantly (\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      \u2009\u2264\u20090.01) increased the MUC5AC level in the tear fluid. Although no drug affected the number of PAS-positive cells, the ratio of the PAS-positive area decreased significantly (\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      \u2009\u2264\u20090.01) only in the diquafosol group.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions:<\/jats:title>\n                    <jats:p>These data indicated that more PAS-positive MUC5AC was released into the tear fluid from the goblet cells by diquafosol than by rebamipide. There is a difference in the induction pattern of MUC5AC into the tears from the goblet cells between these eye drops.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1089\/jop.2016.0092","type":"journal-article","created":{"date-parts":[[2017,3,27]],"date-time":"2017-03-27T17:46:04Z","timestamp":1490636764000},"page":"493-497","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":26,"title":["Comparison of Short-Term Effects of Diquafosol and Rebamipide on Mucin 5AC Level on the Rabbit Ocular Surface"],"prefix":"10.1177","volume":"33","author":[{"given":"Yuichi","family":"Hori","sequence":"first","affiliation":[{"name":"Toho University","place":["Japan"]},{"name":"Toho University Sakura Medical Center","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomofumi","family":"Kageyama","sequence":"additional","affiliation":[{"name":"Santen Pharmaceutical Co., Ltd.","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asuka","family":"Sakamoto","sequence":"additional","affiliation":[{"name":"Santen Pharmaceutical Co., Ltd.","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomoaki","family":"Shiba","sequence":"additional","affiliation":[{"name":"Toho University","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masatsugu","family":"Nakamura","sequence":"additional","affiliation":[{"name":"Santen Pharmaceutical Co., Ltd.","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takatoshi","family":"Maeno","sequence":"additional","affiliation":[{"name":"Toho University Sakura Medical Center","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2017,3,27]]},"reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1542-0124(12)70081-2"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-4835(03)00204-5"},{"key":"e_1_3_2_4_2","first-page":"96","article-title":"Improvement of corneal barrier function by the P2Y2 agonist INS365 in a rat dry eye model","volume":"42","author":"Fujihara T.","year":"2001","unstructured":"Fujihara T., , Murakami T., , Fujita H., , Nakamura M., , and Nakata K. 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