{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T15:52:11Z","timestamp":1777909931151,"version":"3.51.4"},"reference-count":37,"publisher":"SAGE Publications","issue":"6","license":[{"start":{"date-parts":[[2020,7,1]],"date-time":"2020-07-01T00:00:00Z","timestamp":1593561600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Journal of Ocular Pharmacology and Therapeutics"],"published-print":{"date-parts":[[2020,7,1]]},"abstract":"<jats:sec>\n                    <jats:title>Purpose:<\/jats:title>\n                    <jats:p>Drug delivery to treat ocular diseases still is a challenge in ophthalmology. One way to achieve drug delivery that is investigated currently is topical administration of drug-loaded polymeric nanoparticles (NPs) that are able to penetrate ocular barriers. The purpose of this study was optimal preparation of NPs made from pseudo-proteins and evaluation of their ability to penetrate ocular tissues.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>\n                      Biodegradable NPs of various types were prepared by nanoprecipitation of pseudo-protein composed of\n                      <jats:sc>l<\/jats:sc>\n                      -leucine (L), 1,6-hexanediol (6), and sebacic acid (8) (8L6). Arginine-based cationic polyester amides 8R6 and comb-like polyester amide containing lateral PEG-2000 chains along with 8L6 anchoring fragments in the backbones were used to construct positively charged and PEGylated NPs. They were loaded with fluorescein diacetate (FDA) or rhodamine 6G (Rh6G) as fluorescent probes. Suspensions of the NPs were given to cultivated microglial cells and retinal pigment epithelial (RPE) cells as well as topically on eyes of C57BL\/6 mice. Penetration of NPs into the eyes was checked by fluorescence analysis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>NPs were prepared, and their properties were characterized. Cultured microglial cells and RPE cells took up the NPs. After topical administration, penetration of NPs into the cornea of the eyes was clearly seen. Small amounts of fluorescent dyes were also found in the lens, the retina, and the sclera depending on the type of NPs.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions:<\/jats:title>\n                    <jats:p>The results showed that the new NPs penetrate ocular tissues after topical administration and are internalized by the cells. This raises confidence that the NPs may be useful carriers of therapeutic agents for ocular delivery.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1089\/jop.2019.0148","type":"journal-article","created":{"date-parts":[[2020,7,8]],"date-time":"2020-07-08T10:41:25Z","timestamp":1594204885000},"page":"421-432","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":5,"title":["Biodegradable Nanoparticles Based on Pseudo-Proteins Show Promise as Carriers for Ophthalmic Drug Delivery"],"prefix":"10.1177","volume":"36","author":[{"given":"Wenliang","family":"Zhang","sequence":"first","affiliation":[{"name":"Research Lab of the Department of Ophthalmology, University of M\u00fcnster Medical School, M\u00fcnster, Germany."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Temur","family":"Kantaria","sequence":"additional","affiliation":[{"name":"Institute of Chemistry and Molecular Engineering, Agricultural University of Georgia, Tbilisi, Georgia."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yahan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Research Lab of the Department of Ophthalmology, University of M\u00fcnster Medical School, M\u00fcnster, Germany."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tengiz","family":"Kantaria","sequence":"additional","affiliation":[{"name":"Institute of Chemistry and Molecular Engineering, Agricultural University of Georgia, Tbilisi, Georgia."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sophio","family":"Kobauri","sequence":"additional","affiliation":[{"name":"Institute of Chemistry and Molecular Engineering, Agricultural University of Georgia, Tbilisi, Georgia."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Tugushi","sequence":"additional","affiliation":[{"name":"Institute of Chemistry and Molecular Engineering, Agricultural University of Georgia, Tbilisi, Georgia."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Viktoria","family":"Br\u00fccher","sequence":"additional","affiliation":[{"name":"Research Lab of the Department of Ophthalmology, University of M\u00fcnster Medical School, M\u00fcnster, Germany."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ramaz","family":"Katsarava","sequence":"additional","affiliation":[{"name":"Institute of Chemistry and Molecular Engineering, Agricultural University of Georgia, Tbilisi, Georgia."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicole","family":"Eter","sequence":"additional","affiliation":[{"name":"Research Lab of the Department of Ophthalmology, University of M\u00fcnster Medical School, M\u00fcnster, Germany."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Heiduschka","sequence":"additional","affiliation":[{"name":"Research Lab of the Department of Ophthalmology, University of M\u00fcnster Medical School, M\u00fcnster, Germany."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2020,7,24]]},"reference":[{"key":"e_1_3_3_2_2","first-page":"844","article-title":"Global data on visual impairment in the year 2002","volume":"82","author":"Resnikoff S.","year":"2004","unstructured":"Resnikoff S., , Pascolini D., , Etya'ale D., et al. 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