{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T15:52:56Z","timestamp":1777909976809,"version":"3.51.4"},"reference-count":52,"publisher":"SAGE Publications","issue":"6","license":[{"start":{"date-parts":[[2022,4,4]],"date-time":"2022-04-04T00:00:00Z","timestamp":1649030400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Journal of Ocular Pharmacology and Therapeutics"],"published-print":{"date-parts":[[2022,8,1]]},"abstract":"<jats:sec>\n                    <jats:title>Purpose:<\/jats:title>\n                    <jats:p>To compare a novel, sustained release formulation and a bolus injection of a targeted nanocarrier for the ability to specifically deplete cells responsible for the development of posterior capsule opacification (PCO) in week-long, dynamic cell cultures.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>\n                      A novel, injectable, thermosensitive poly(D,L-lactic-co-glycolic acid)-b-poly(ethylene glycol)-b-poly(D,L-lactic-co-glycolic acid) (PLGA-PEG-PLGA) triblock copolymer hydrogel was engineered for the sustained release of targeted, nucleic acid nanocarriers loaded with cytotoxic doxorubicin (G8:3DNA:Dox). Human rhabdomyosarcoma (RD) cells were used due to their expression of brain-specific angiogenesis inhibitor 1 (BAI1), a specific marker for the myofibroblasts responsible for PCO. Under constant media flow, nanocarriers were injected into cell cultures as either a bolus or within the hydrogel. Cells were fixed and stained every other day for 7 days to compare targeted depletion of BAI1\n                      <jats:sup>+<\/jats:sup>\n                      cells.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      The formulation transitions to a gel at physiological temperatures, is optically clear, noncytotoxic, and can release G8:3DNA:Dox nanocarriers for up to 4 weeks. In RD cell cultures, G8:3DNA:Dox nanocarriers specifically eliminated BAI1\n                      <jats:sup>+<\/jats:sup>\n                      cells. The bolus nanocarrier dose showed significantly reduced cell depletion overtime, while the sustained release of nanocarriers showed increased cell depletion over time. By day 7, &lt;2% of BAI1\n                      <jats:sup>+<\/jats:sup>\n                      cells were depleted by the bolus injection and 74.2% BAI1\n                      <jats:sup>+<\/jats:sup>\n                      cells were targeted by the sustained release of nanocarriers.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions:<\/jats:title>\n                    <jats:p>The sustained release of nanocarriers from the hydrogel allows for improved therapeutic delivery in a dynamic system. This method can offer a more effective and efficient method of prophylactically treating PCO after cataract surgery.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1089\/jop.2021.0111","type":"journal-article","created":{"date-parts":[[2022,4,4]],"date-time":"2022-04-04T11:16:37Z","timestamp":1649070997000},"page":"404-411","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Sustained Release of Antibody-Conjugated DNA Nanocarriers from a Novel Injectable Hydrogel for Targeted Cell Depletion to Treat Cataract Posterior Capsule Opacification"],"prefix":"10.1177","volume":"38","author":[{"given":"Laura L.","family":"Osorno","sequence":"first","affiliation":[{"name":"Biomimetic and Biohybrid Materials, Biomedical Devices, and Drug Delivery Laboratories, Department of Biomedical Engineering, Rowan University, Glassboro, New Jersey, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert J.","family":"Mosley","sequence":"additional","affiliation":[{"name":"Biomimetic and Biohybrid Materials, Biomedical Devices, and Drug Delivery Laboratories, Department of Biomedical Engineering, Rowan University, Glassboro, New Jersey, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patricia L.","family":"Poley","sequence":"additional","affiliation":[{"name":"Biomimetic and Biohybrid Materials, Biomedical Devices, and Drug Delivery Laboratories, Department of Biomedical Engineering, Rowan University, Glassboro, New Jersey, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jessica","family":"Bowers","sequence":"additional","affiliation":[{"name":"Genisphere, LLC, Hatfield, Pennsylvania, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Grzegorz","family":"Gorski","sequence":"additional","affiliation":[{"name":"Philadelphia College of Osteopathic Medicine, Philadelphia, Pennsylvania, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jacquelyn","family":"Gerhart","sequence":"additional","affiliation":[{"name":"Philadelphia College of Osteopathic Medicine, Philadelphia, Pennsylvania, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert","family":"Getts","sequence":"additional","affiliation":[{"name":"Code Biotherapeutics, Pennsylvania, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mindy","family":"George-Weinstein","sequence":"additional","affiliation":[{"name":"Philadelphia College of Osteopathic Medicine, Philadelphia, Pennsylvania, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7038-7042","authenticated-orcid":false,"given":"Mark E.","family":"Byrne","sequence":"additional","affiliation":[{"name":"Biomimetic and Biohybrid Materials, Biomedical Devices, and Drug Delivery Laboratories, Department of Biomedical Engineering, Rowan University, Glassboro, New Jersey, USA."},{"name":"Department of Chemical Engineering, Rowan University, Glassboro, New Jersey, USA."},{"name":"OcuMedic, Inc., Mullica Hill, New Jersey, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2022,4,4]]},"reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S2214-109X(20)30489-7"},{"key":"e_1_3_4_3_2","first-page":"17","article-title":"Vision 2020: the cataract challenge","volume":"13","author":"Foster A.","year":"2000","unstructured":"Foster A. 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