{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T23:43:12Z","timestamp":1781739792653,"version":"3.54.5"},"reference-count":61,"publisher":"SAGE Publications","issue":"10","license":[{"start":{"date-parts":[[2022,12,1]],"date-time":"2022-12-01T00:00:00Z","timestamp":1669852800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Journal of Ocular Pharmacology and Therapeutics"],"published-print":{"date-parts":[[2022,12,1]]},"abstract":"<jats:sec>\n                    <jats:title>Purpose:<\/jats:title>\n                    <jats:p>To characterize the impact of corneal cold storage (CS) on the endothelial apical junctional complex (AJC).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>Porcine corneas were held in CS (4\u00b0C; 1\u20137 days) with Cornisol\u2122 preservation medium supplemented with epothilone B (EpoB; microtubule stabilizer; 100\u2009nM), SB-203580 (p38 mitogen-activated protein [MAP] kinase inhibitor; 20\u2009\u03bcM), or antioxidants (quercetin, 100\u2009\u03bcM; vitamin E, 1\u2009mM; deferoxamine, an iron chelator, 10\u2009mM). After CS termination, the damage to endothelial AJC was characterized by imaging perijunctional actomyosin ring (PAMR) and zonula occludens (ZO-1). The effects of EpoB and SB-203580 were characterized by imaging microtubules. The loss in the barrier function was assessed in cultured cells grown on biotin-coated gelatin by permeability to fluorescein isothiocyanate (FITC)-avidin. The accumulation of reactive oxygen species (ROS), altered mitochondrial membrane potential (MMP), lipid peroxidation, and lactate dehydrogenase (LDH) release were also determined in response to CS.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>CS led to the loss of microtubules, destruction of PAMR, and breakdown of ZO-1 in the endothelium. The severity of damage increased when CS was prolonged. Although rewarming of the tissue increased the damage, the effect was marginal. CS also induced accumulation of ROS, alteration in MMP, lipid peroxidation, enhanced LDH release, and increased permeability to FITC-avidin. These changes were opposed by EpoB, SB-203580, and antioxidants.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>Corneal CS destroys AJC of the endothelium, leading to loss of its barrier function. The effects were surmounted by microtubule stabilization, p38 MAP kinase inhibition, and antioxidants. Thus, there is potential for reformulation of the preservation medium to maintain the health of the donor corneal endothelium before transplantation.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1089\/jop.2022.0082","type":"journal-article","created":{"date-parts":[[2022,10,18]],"date-time":"2022-10-18T11:13:21Z","timestamp":1666091601000},"page":"664-681","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":6,"title":["Role of Oxidative Stress in the Disruption of the Endothelial Apical Junctional Complex During Corneal Cold Storage"],"prefix":"10.1177","volume":"38","author":[{"given":"M.Y.","family":"Thanuja","sequence":"first","affiliation":[{"name":"Bio-INvENT Lab, Department of Chemical Engineering, Siddaganga Institute of Technology, Tumakuru, India."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0732-0341","authenticated-orcid":false,"given":"Sudhir H.","family":"Ranganath","sequence":"additional","affiliation":[{"name":"Bio-INvENT Lab, Department of Chemical Engineering, Siddaganga Institute of Technology, Tumakuru, India."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sangly P.","family":"Srinivas","sequence":"additional","affiliation":[{"name":"School of Optometry, Indiana University, Bloomington, Indiana, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2022,12,2]]},"reference":[{"key":"e_1_3_4_2_2","article-title":"The effect of donor age on corneal transplantation outcome results of the cornea donor study","volume":"115","author":"Cornea Donor Study Investigator G","year":"2008","unstructured":"Cornea Donor Study Investigator G, , gal RL, , Dontchev M, et al. 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