{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T15:53:42Z","timestamp":1777910022295,"version":"3.51.4"},"reference-count":49,"publisher":"SAGE Publications","issue":"6","license":[{"start":{"date-parts":[[2024,8,1]],"date-time":"2024-08-01T00:00:00Z","timestamp":1722470400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Journal of Ocular Pharmacology and Therapeutics"],"published-print":{"date-parts":[[2024,8,1]]},"abstract":"<jats:sec>\n                    <jats:title>Purpose:<\/jats:title>\n                    <jats:p>To explore the effects of the tight junction protein zonula occludens 1 (ZO-1) on experimental corneal neovascularization (CNV).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>CNV models were established in the left eyes of BALB\/c mice using NaOH. Anti-ZO-1 neutralizing antibody was topically applied to the burnt corneas after modeling thrice a day for 1 week. CD31 expression was analyzed to calculate the ratio of CNV number to area using a corneal whole-mount fluorescent immunohistochemical assay. Messenger ribonucleic acid (mRNA) and protein expression levels of ZO-1, vascular endothelial growth factor (VEGF), interleukin (IL)-1\u03b2, IL-6, IL-8, IL-18, monocyte chemoattractant protein-1 (MCP-1), tumor necrosis factor alpha (TNF-\u03b1), phosphorylated protein kinase C (pPKC), and clusterin in burned corneas were detected by reverse transcriptase polymerase chain reaction (PCR) and western blot analyses. Infiltration of neutrophils, macrophages, and progenitor cells was examined by flow cytometry.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>CNV was obviously greater in 45 s than in 15 s alkali injury group. In another experiment, CNV was obviously greater in the ZO-1 antibody group than in the vehicle-treated group. Corneal mRNA and protein expression levels of VEGF, IL-1\u03b2, IL-6, IL-8, IL-18, and MCP-1 were significantly higher in the ZO-1 antibody group than in the control group. Infiltration of neutrophils, macrophages, and progenitor cells was significantly greater in the ZO-1 antibody group than in the control group. TNF-\u03b1 expression was much higher in 45 s than in 15 s alkali injury group. However, protein expression of pPKC and clusterin was much lower in 45 s than in 15 s alkali injury group.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions:<\/jats:title>\n                    <jats:p>Anti-ZO-1 neutralizing antibody-treated mice exhibited enhanced alkali-induced CNV through enhanced intracorneal infiltration of progenitor and inflammatory cells.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1089\/jop.2023.0162","type":"journal-article","created":{"date-parts":[[2024,8,22]],"date-time":"2024-08-22T10:51:28Z","timestamp":1724323888000},"page":"379-388","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":7,"title":["Inhibition of Experimental Corneal Neovascularization by the Tight Junction Protein ZO-1"],"prefix":"10.1177","volume":"40","author":[{"given":"Qingying","family":"Yao","sequence":"first","affiliation":[{"name":"Department of Ophthalmology, The First Affiliated Hospital of Soochow University, Suzhou, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongya","family":"Wu","sequence":"additional","affiliation":[{"name":"Jiangsu Institute of Clinical Immunology, The First Affiliated Hospital of Soochow University, Suzhou, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hang","family":"Ren","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, The First Affiliated Hospital of Soochow University, Suzhou, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiufa","family":"Cao","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, The First Affiliated Hospital of Soochow University, Suzhou, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying","family":"Shao","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, The First Affiliated Hospital of Soochow University, Suzhou, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gaoqin","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, The First Affiliated Hospital of Soochow University, Suzhou, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peirong","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, The First Affiliated Hospital of Soochow University, Suzhou, China."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2024,8,19]]},"reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1136\/bjophthalmol-2020-316705"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1542-0124(12)70308-7"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2020.108363"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2021.108434"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.22336\/rjo.2019.4"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.3109\/08820538.2011.588652"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1136\/bjophthalmol-2015-307901"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.3390\/cells11203247"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.preteyeres.2020.100876"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.2174\/1389450121666200319111710"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.3390\/biom13050831"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.294967"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.addr.2021.02.008"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/nyas.14798"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm.2016.80"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2020.08.004"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.3892\/or.2014.3219"},{"issue":"14","key":"e_1_3_4_19_2","first-page":"2621","article-title":"Vascular endothelial growth factor increases GEnC permeability by affecting the distributions of occludin, ZO-1 and tight juction assembly","volume":"19","author":"Zhang L","year":"2015","unstructured":"Zhang L, , Liu H, , Peng YM, et al. Vascular endothelial growth factor increases GEnC permeability by affecting the distributions of occludin, ZO-1 and tight juction assembly. Eur Rev Med Pharmacol Sci, 2015; 19(14):2621\u20132627.","journal-title":"Eur Rev Med Pharmacol Sci"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E07-12-1215"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2023.1150017"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a029181"},{"key":"e_1_3_4_23_2","first-page":"2129","article-title":"Critical role of SDF-1alpha-induced progenitor cell recruitment and macrophage VEGF production in the experimental corneal neovascularization","volume":"17","author":"Liu G","year":"2011","unstructured":"Liu G, , Lu P, , Li L, et al. Critical role of SDF-1alpha-induced progenitor cell recruitment and macrophage VEGF production in the experimental corneal neovascularization. Mol Vis, 2011; 17:2129\u20132138.","journal-title":"Mol Vis"},{"key":"e_1_3_4_24_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.103.3.755"},{"key":"e_1_3_4_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcdb.2014.08.011"},{"key":"e_1_3_4_26_2","doi-asserted-by":"publisher","DOI":"10.3390\/cells11233775"},{"key":"e_1_3_4_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamem.2007.07.012"},{"key":"e_1_3_4_28_2","doi-asserted-by":"publisher","DOI":"10.1093\/ibd\/izz266"},{"key":"e_1_3_4_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0169-409X(00)00048-X"},{"key":"e_1_3_4_30_2","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2021.08.047"},{"key":"e_1_3_4_31_2","doi-asserted-by":"publisher","DOI":"10.1039\/C9FO01718F"},{"key":"e_1_3_4_32_2","doi-asserted-by":"publisher","DOI":"10.1086\/380418"},{"key":"e_1_3_4_33_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0063674"},{"issue":"6","key":"e_1_3_4_34_2","first-page":"1083","article-title":"Anti-apoptosis effects of vascular endothelial cadherin in experimental corneal neovascularization","volume":"8","author":"Liu GQ","year":"2015","unstructured":"Liu GQ, , Wu HY, , Xu J, et al. Anti-apoptosis effects of vascular endothelial cadherin in experimental corneal neovascularization. Int J Ophthalmol, 2015; 8(6):1083\u20131088.","journal-title":"Int J Ophthalmol"},{"key":"e_1_3_4_35_2","doi-asserted-by":"publisher","DOI":"10.1080\/02713683.2016.1196705"},{"key":"e_1_3_4_36_2","doi-asserted-by":"publisher","DOI":"10.1080\/09273948.2020.1870148"},{"key":"e_1_3_4_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtos.2018.06.004"},{"key":"e_1_3_4_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2023.109539"},{"key":"e_1_3_4_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(01)02110-X"},{"key":"e_1_3_4_40_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10456-016-9497-6"},{"key":"e_1_3_4_41_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-010-0476-6"},{"key":"e_1_3_4_42_2","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.03-0342"},{"key":"e_1_3_4_43_2","doi-asserted-by":"publisher","DOI":"10.1097\/ICO.0000000000000071"},{"key":"e_1_3_4_44_2","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.11-8722"},{"key":"e_1_3_4_45_2","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.16-19702"},{"key":"e_1_3_4_46_2","doi-asserted-by":"publisher","DOI":"10.1172\/jci.insight.168142"},{"key":"e_1_3_4_47_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2018.02.035"},{"key":"e_1_3_4_48_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2010.05.034"},{"key":"e_1_3_4_49_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2017.11.018"},{"key":"e_1_3_4_50_2","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202302174"}],"container-title":["Journal of Ocular Pharmacology and Therapeutics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1089\/jop.2023.0162","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/jop.2023.0162","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/jop.2023.0162","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T14:46:05Z","timestamp":1777646765000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1089\/jop.2023.0162"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,8,1]]},"references-count":49,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2024,8,1]]}},"alternative-id":["10.1089\/jop.2023.0162"],"URL":"https:\/\/doi.org\/10.1089\/jop.2023.0162","relation":{},"ISSN":["1080-7683","1557-7732"],"issn-type":[{"value":"1080-7683","type":"print"},{"value":"1557-7732","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,8,1]]}}}