{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T03:17:16Z","timestamp":1790738236569,"version":"4.1.0"},"reference-count":31,"publisher":"SAGE Publications","issue":"8","license":[{"start":{"date-parts":[[2024,10,1]],"date-time":"2024-10-01T00:00:00Z","timestamp":1727740800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Journal of Ocular Pharmacology and Therapeutics"],"published-print":{"date-parts":[[2024,10,1]]},"abstract":"<jats:sec>\n                    <jats:title>Purpose:<\/jats:title>\n                    <jats:p>\n                      Corneal fibroblasts are involved in the wound healing of the cornea with proliferation, migration, and differentiation processes. Coenzyme Q10 (CoQ10) and vitamin E can enhance corneal wound healing when applied after a corneal lesion as an eye drop. Thus, this study was performed to determine the potential efficiency of a CoQ10 ophthalmical solution containing a CoQ10 and vitamin E D-\u03b1-tocopherol polyethylene glycol 1000 succinate (TPGS)-derived formulation in human corneal fibroblasts (HCFs)\n                      <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>\n                      Primary HCFs were obtained from cadaveric corneal tissue, and cell viability was determined using MTT assay at 24 and 72 h. Cell migration was evaluated using an\n                      <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                      wound healing assay, and mRNA expressions of collagen type I (COL-I), collagen type III (COL-III), lumican, hyaluronan, matrix metalloproteinase (MMP)-1, MMP-2, MMP-9, tissue inhibitors of MMP (TIMP)-1, TIMP-2, interleukin (IL)-1\u03b2, IL-6, IL-8, and IL-10 were assessed using reverse transcription polymerase chain reaction at 24 and 72 h.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      At various concentrations of CoQ10 ophthalmical solution (CoQ10-os), cell viability and wound healing rates of HCFs increased compared with the control group. The expressions of\n                      <jats:italic toggle=\"yes\">COL-I<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">COL-III<\/jats:italic>\n                      , lumican, and hyaluronan were increased by CoQ10-os, whereas those of\n                      <jats:italic toggle=\"yes\">MMP-1<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">MMP-2<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">MMP-9<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">TIMP-1<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">TIMP-2<\/jats:italic>\n                      , and\n                      <jats:italic toggle=\"yes\">TIMP-3<\/jats:italic>\n                      were not affected by CoQ10-os at 24 and 72 h. In treating HCFs with a CoQ10-os medium,\n                      <jats:italic toggle=\"yes\">IL-1\u03b2<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">IL-6<\/jats:italic>\n                      , and\n                      <jats:italic toggle=\"yes\">IL-8<\/jats:italic>\n                      decreased, whereas\n                      <jats:italic toggle=\"yes\">IL-10<\/jats:italic>\n                      was significantly increased in a time- and dose-dependent manner.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions:<\/jats:title>\n                    <jats:p>The findings indicate that CoQ10 and vitamin E-TPGS are potent regulators of the bioactivity of HCFs, thus supporting their potential application as ophthalmical solutions in therapies aimed at the fast regeneration of damaged cornea tissues.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1089\/jop.2024.0050","type":"journal-article","created":{"date-parts":[[2024,7,8]],"date-time":"2024-07-08T11:34:40Z","timestamp":1720438480000},"page":"494-503","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":1,"title":["Coenzyme Q10 and Vitamin E Regulate the Bioactivity of Human Corneal Fibroblast Cells"],"prefix":"10.1177","volume":"40","author":[{"given":"Kursad Ramazan","family":"Zor","sequence":"first","affiliation":[{"name":"Department of Ophthalmology, Faculty of Medicine, Ni\u011fde \u00d6mer Halisdemir University, Ni\u011fde, T\u00fcrkiye."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ugur","family":"Y\u0131lmaz","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Faculty of Medicine, Pamukkale University, Denizli, T\u00fcrkiye."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8641-2844","authenticated-orcid":false,"given":"Serife Buket","family":"Bozkurt","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Medicine, Ni\u011fde \u00d6mer Halisdemir University, Ni\u011fde, T\u00fcrkiye."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2024,10,15]]},"reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.24272\/j.issn.2095-8137.2020.109"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2020.108062"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2020.108229"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2014.09.013"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.3390\/cells10030687"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2015.02.016"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112.401844"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.2741\/4231"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.3928\/1081-597X-20020301-06"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.14-15306"},{"issue":"8","key":"e_1_3_4_12_2","first-page":"S66","article-title":"The function of coenzyme Q in mitochondria","volume":"71","author":"Lenaz G","year":"1993","unstructured":"Lenaz G, , Fato R, , Castelluccio C, et al. 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