{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T22:21:02Z","timestamp":1792102862619,"version":"4.4.0"},"reference-count":34,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[2025,6,1]],"date-time":"2025-06-01T00:00:00Z","timestamp":1748736000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Journal of Ocular Pharmacology and Therapeutics"],"published-print":{"date-parts":[[2025,6,1]]},"abstract":"<jats:sec>\n                    <jats:title>Purpose:<\/jats:title>\n                    <jats:p>Nanoparticle-based drug delivery systems offer a promising approach for overcoming the challenges of ocular drug delivery. Our study evaluated the biodistribution and potential targeting of reconstituted high-density lipoprotein nanoparticles (rHDL NPs) loaded with near-infrared dye IR780 to retinal ganglion cells (RGCs) and optic nerve head astrocytes (ONHAs) as a model for neuroprotective drug delivery in glaucoma.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>\n                      A stable rHDL-payload complex was formulated using IR780, phosphatidylcholine, and apolipoprotein A-I (Apo A-I) by using a novel preparation method. Fluorescent rHDL (rHDL-IR780) was assessed for cellular uptake in primary human ONHAs\n                      <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                      , whereas scavenger receptor class B1 (SR-B1) expression was confirmed by Western blot. Receptor-mediated uptake was examined by SR-B1 receptor blocking.\n                      <jats:italic toggle=\"yes\">Ex vivo<\/jats:italic>\n                      biodistribution was evaluated by intravitreal injection of rHDL into postmortem human donor eyes.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      Spectroscopic analysis confirmed IR780 encapsulation in rHDL NPs. Blocking SR-B1 receptors significantly reduced IR780 uptake by ONHAs, supporting an SR-B1-mediated delivery mechanism, in addition to confirming SR-B1 expression in human retinal lysates. In\n                      <jats:italic toggle=\"yes\">ex vivo<\/jats:italic>\n                      experiments, 4 h postinjection, IR780 localized in the retinal nerve fiber and ganglion cell layers. By 24 h, IR780 penetrated deeper retinal layers, achieving RGC uptake.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions:<\/jats:title>\n                    <jats:p>Our findings demonstrate that rHDL NPs facilitate targeted delivery to retinal tissues through an Apo A-I\/SR-B1 pathway, overcoming ocular barriers to reach RGCs. This study supports the potential of rHDL NPs as a platform for neuroprotective drug delivery to treat glaucoma, enhancing both pharmacokinetics and targeted cellular uptake.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1089\/jop.2024.0191","type":"journal-article","created":{"date-parts":[[2025,4,18]],"date-time":"2025-04-18T04:36:26Z","timestamp":1744950986000},"page":"281-289","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":3,"title":["Biodistribution of Reconstituted High-Density Lipoprotein Nanoparticles for Targeted Delivery to Retinal Ganglion Cells"],"prefix":"10.1177","volume":"41","author":[{"given":"R. Max","family":"Petty","sequence":"first","affiliation":[{"name":"Department of Pharmacology and Neuroscience, College of Biomedical and Translational Sciences, University of North Texas Health Science Center, Fort Worth, Texas, USA."},{"name":"North Texas Eye Research Institute, Fort Worth, Texas, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rajiv S.","family":"Rangan","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Neuroscience, College of Biomedical and Translational Sciences, University of North Texas Health Science Center, Fort Worth, Texas, USA."},{"name":"North Texas Eye Research Institute, Fort Worth, Texas, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stacy","family":"Curry","sequence":"additional","affiliation":[{"name":"North Texas Eye Research Institute, Fort Worth, Texas, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Calvin D.","family":"Brooks","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Neuroscience, College of Biomedical and Translational Sciences, University of North Texas Health Science Center, Fort Worth, Texas, USA."},{"name":"North Texas Eye Research Institute, Fort Worth, Texas, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nirupama","family":"Sabnis","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology and Genetics, College of Biomedical and Translational Sciences, University of North Texas Health Science Center, Fort Worth, Texas, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abbot F.","family":"Clark","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Neuroscience, College of Biomedical and Translational Sciences, University of North Texas Health Science Center, Fort Worth, Texas, USA."},{"name":"North Texas Eye Research Institute, Fort Worth, Texas, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andras G.","family":"Lacko","sequence":"additional","affiliation":[{"name":"Department of Physiology and Anatomy, College of Biomedical and Translational Sciences, University of North Texas Health Science Center, Fort Worth, Texas, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raghu R.","family":"Krishnamoorthy","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Neuroscience, College of Biomedical and Translational Sciences, University of North Texas Health Science Center, Fort Worth, Texas, USA."},{"name":"North Texas Eye Research Institute, Fort Worth, Texas, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2025,6,2]]},"reference":[{"key":"e_1_3_5_2_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1095833"},{"key":"e_1_3_5_3_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms242015352"},{"key":"e_1_3_5_4_2","doi-asserted-by":"publisher","DOI":"10.30574\/gscbps.2019.6.3.0030"},{"key":"e_1_3_5_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2019.02.029"},{"key":"e_1_3_5_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2021.02.017"},{"key":"e_1_3_5_7_2","doi-asserted-by":"publisher","DOI":"10.1186\/s40662-022-00298-y"},{"key":"e_1_3_5_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.survophthal.2021.12.003"},{"key":"e_1_3_5_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1350-9462(98)00014-7"},{"key":"e_1_3_5_10_2","doi-asserted-by":"publisher","DOI":"10.1186\/1755-8794-2-24"},{"issue":"4","key":"e_1_3_5_11_2","first-page":"2045","article-title":"High density lipoprotein complexes as delivery vehicles for anticancer drugs","volume":"22","author":"Lacko AG","year":"2002","unstructured":"Lacko AG, , Nair M, , Paranjape S, et al. 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