{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T16:06:49Z","timestamp":1787933209788,"version":"build-2784847793"},"reference-count":48,"publisher":"SAGE Publications","issue":"7","license":[{"start":{"date-parts":[[2025,6,11]],"date-time":"2025-06-11T00:00:00Z","timestamp":1749600000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Journal of Ocular Pharmacology and Therapeutics"],"published-print":{"date-parts":[[2025,9,1]]},"abstract":"<jats:sec>\n                    <jats:title>Purpose:<\/jats:title>\n                    <jats:p>To investigate the role of autologous serum (AS) in alleviating hyperosmolarity-induced corneal epitheliopathy via the ferroptosis pathway.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>\n                      AS was extracted and prepared from patients with Sj\u00f6gren\u2019s syndrome. An\n                      <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                      hyperosmotic dry eye model was established by exposing HCE-T cells to 90 mM NaCl, followed by treatment with AS or ferroptosis inhibitors. Protein expression was analyzed using Western blotting. Intracellular Fe\n                      <jats:sup>2+<\/jats:sup>\n                      and reactive oxygen species (ROS) levels were determined by FerroOrange and 2\u2019,7\u2019-Dichlorodihydrofluorescein diacetate (H2DCFDA) fluorescent probes, and cellular malondialdehyde and glutathione levels were measured using kits. Transmission electron microscopy was utilized to examine mitochondrial ultrastructure.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      Under hyperosmotic conditions, HCE-T cells exhibited increased lipid peroxidation and iron accumulation, accompanied by significant alterations in the expression of ferroptosis-related markers and characteristic changes in mitochondrial morphology, which indicated the induction of ferroptosis. Among the tested concentrations, 20% and 50% AS demonstrated notable therapeutic effects, significantly enhancing cell viability and migration, reducing ROS and Fe\n                      <jats:sup>2+<\/jats:sup>\n                      levels, and alleviating mitochondrial damage. Furthermore, AS effectively inhibited ferroptosis through the Xc\n                      <jats:sup>\u2212<\/jats:sup>\n                      \/glutathione peroxidase 4 (GPX4) pathway.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions:<\/jats:title>\n                    <jats:p>\n                      AS reduced lipid peroxidation levels in HCE-T cells under hyperosmolarity and inhibited ferroptosis through the Xc\n                      <jats:sup>\u2212<\/jats:sup>\n                      \/GPX4 pathway.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1089\/jop.2025.0025","type":"journal-article","created":{"date-parts":[[2025,6,11]],"date-time":"2025-06-11T04:13:03Z","timestamp":1749615183000},"page":"397-407","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":5,"title":["Autologous Serum from Sj\u00f6gren\u2019s Syndrome Patients Mitigates Ferroptosis in Hyperosmolarity-Induced Corneal Epitheliopathy"],"prefix":"10.1177","volume":"41","author":[{"given":"Guanghong","family":"Chu","sequence":"first","affiliation":[{"name":"Chongqing Branch (Municipality Division) of National Clinical Research Center for Ocular Diseases, Chongqing Key Laboratory of Ophthalmology, Chongqing Eye Institute, The First Affiliated Hospital of Chongqing Medical University, Chongqing, P. R. China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiao","family":"Wang","sequence":"additional","affiliation":[{"name":"Chongqing Branch (Municipality Division) of National Clinical Research Center for Ocular Diseases, Chongqing Key Laboratory of Ophthalmology, Chongqing Eye Institute, The First Affiliated Hospital of Chongqing Medical University, Chongqing, P. R. China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zihuan","family":"Wang","sequence":"additional","affiliation":[{"name":"Chongqing Branch (Municipality Division) of National Clinical Research Center for Ocular Diseases, Chongqing Key Laboratory of Ophthalmology, Chongqing Eye Institute, The First Affiliated Hospital of Chongqing Medical University, Chongqing, P. R. China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyu","family":"Cai","sequence":"additional","affiliation":[{"name":"Chongqing Branch (Municipality Division) of National Clinical Research Center for Ocular Diseases, Chongqing Key Laboratory of Ophthalmology, Chongqing Eye Institute, The First Affiliated Hospital of Chongqing Medical University, Chongqing, P. R. China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siyuan","family":"Shi","sequence":"additional","affiliation":[{"name":"Chongqing Branch (Municipality Division) of National Clinical Research Center for Ocular Diseases, Chongqing Key Laboratory of Ophthalmology, Chongqing Eye Institute, The First Affiliated Hospital of Chongqing Medical University, Chongqing, P. R. China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiping","family":"Qing","sequence":"additional","affiliation":[{"name":"Chongqing Branch (Municipality Division) of National Clinical Research Center for Ocular Diseases, Chongqing Key Laboratory of Ophthalmology, Chongqing Eye Institute, The First Affiliated Hospital of Chongqing Medical University, Chongqing, P. R. China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Zhong","sequence":"additional","affiliation":[{"name":"Department of Combination of Chinese and Western Medicine, The First Affiliated Hospital of Chongqing Medical University, Chongqing, P. R. 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