{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T19:17:51Z","timestamp":1777922271310,"version":"3.51.4"},"reference-count":12,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2013,8,1]],"date-time":"2013-08-01T00:00:00Z","timestamp":1375315200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Monoclonal Antibodies in Immunodiagnosis &amp; Immunotherapy"],"published-print":{"date-parts":[[2013,8]]},"abstract":"<jats:p>\n                    <jats:italic toggle=\"yes\">MUDENG<\/jats:italic>\n                    (\n                    <jats:italic toggle=\"yes\">mu-2-related death-inducing gene<\/jats:italic>\n                    ,\n                    <jats:italic toggle=\"yes\">MuD<\/jats:italic>\n                    ) encodes a predicted \u223c54-kDa protein in humans, considered to be involved in trafficking proteins from endosomes toward other membranous compartments as well as in inducing cell death. Here we report on the generation of a mouse monoclonal antibody (MAb) against the middle domain of human (h) MuD. This IgG sub 1 MAb, named M3H9, recognizes residues 244\u2013326 in the middle domain of the MuD protein. Thus, the MuD proteins expressed in an astroglioma cell line and primary astrocytes can be detected by the M3H9 MAb. We showed that M3H9 MAb can be useful in enzyme-linked immunosorbent assay (ELISA) and immunoblot experiments. In addition, M3H9 MAb can detect the expression of the MuD protein in formalin-fixed, paraffin-embedded mouse ovary and uterus tissues. These results indicate that the MuD MAb M3H9 could be useful as a new biomarker of hereditary spastic paraplegia and other related diseases.\n                  <\/jats:p>","DOI":"10.1089\/mab.2013.0015","type":"journal-article","created":{"date-parts":[[2013,8,2]],"date-time":"2013-08-02T10:30:12Z","timestamp":1375439412000},"page":"277-282","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":13,"title":["A Monoclonal Antibody Against Human MUDENG Protein"],"prefix":"10.1177","volume":"32","author":[{"given":"Yadav","family":"Wagley","sequence":"first","affiliation":[{"name":"Konkuk University"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun-Ha","family":"Choi","sequence":"additional","affiliation":[{"name":"Konkuk University"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dimuthu Dhammika","family":"Wickramanayake","sequence":"additional","affiliation":[{"name":"Konkuk University"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Geun-Yeol","family":"Choi","sequence":"additional","affiliation":[{"name":"Konkuk University"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chang-Kyu","family":"Kim","sequence":"additional","affiliation":[{"name":"Konkuk University"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tae-Hyoung","family":"Kim","sequence":"additional","affiliation":[{"name":"Chosun University School of Medicine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jae-Wook","family":"Oh","sequence":"additional","affiliation":[{"name":"Konkuk University"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2013,8]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkf476"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2008.03.139"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.1001170"},{"key":"e_1_3_3_5_2","first-page":"S","article-title":"MUDENG is a mediator of Fas-induced apoptosis signaling and induces BAX-dependent cell death","volume":"2433","author":"Lee K","year":"2009","unstructured":"Lee K, , Kim TH, , Bae J. MUDENG is a mediator of Fas-induced apoptosis signaling and induces BAX-dependent cell death. ASCB Late Abstract Contents, 2009; 2433:S\u2013L29.","journal-title":"ASCB Late Abstract Contents"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.181.5.3167"},{"key":"e_1_3_3_7_2","volume-title":"Molecular Cloning: A Laboratory Manual","author":"Green MR","year":"2001","unstructured":"Green MR, , Sambrook J. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Press: Cold Spring Harbor, NY, 2001."},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4684-4964-8_1"},{"key":"e_1_3_3_9_2","volume-title":"Using Antibodies: A Laboratory Manual","author":"Harlow E","year":"1989","unstructured":"Harlow E, , Lane D. Using Antibodies: A Laboratory Manual. Cold Spring Harbor Laboratory Press: Cold Spring Harbor, NY, 1989."},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200300771"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.1000408"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.1001173"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tips.2008.12.001"}],"container-title":["Monoclonal Antibodies in Immunodiagnosis &amp; Immunotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1089\/mab.2013.0015","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/mab.2013.0015","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/mab.2013.0015","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T20:16:12Z","timestamp":1777666572000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/full\/10.1089\/mab.2013.0015"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,8]]},"references-count":12,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2013,8]]}},"alternative-id":["10.1089\/mab.2013.0015"],"URL":"https:\/\/doi.org\/10.1089\/mab.2013.0015","relation":{},"ISSN":["2167-9436","2167-9436"],"issn-type":[{"value":"2167-9436","type":"print"},{"value":"2167-9436","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,8]]}}}