{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T19:18:25Z","timestamp":1777922305364,"version":"3.51.4"},"reference-count":19,"publisher":"SAGE Publications","issue":"6","license":[{"start":{"date-parts":[[2014,12,1]],"date-time":"2014-12-01T00:00:00Z","timestamp":1417392000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Monoclonal Antibodies in Immunodiagnosis &amp; Immunotherapy"],"published-print":{"date-parts":[[2014,12,1]]},"abstract":"<jats:p>\n                    TB10.4 protein is a member of the ESX family that is necessary for\n                    <jats:italic toggle=\"yes\">Mycobacterium tuberculosis<\/jats:italic>\n                    survival and plays a vital role in mycobacterial pathogenesis. In this study, the gene encoding TB10.4 was cloned into prokaryotic expression vecters pET-30(a) and pGEX-6p-1. The two recombinant proteins His\u2013TB10.4 and GST\u2013TB10.4 were then expressed\n                    <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                    in prokaryotic expression systems to develop monoclonal antibodies (MAbs) against TB10.4 protein. The purified rHis\u2013TB10.4 protein was used to immunize BALB\/c mice, and eight MAbs were produced. An immunoblotting analysis indicated that all these MAbs specifically recognize the TB10.4 protein. These new MAbs provide powerful reagents for further functional research into TB10.4 protein.\n                  <\/jats:p>","DOI":"10.1089\/mab.2014.0039","type":"journal-article","created":{"date-parts":[[2014,12,29]],"date-time":"2014-12-29T15:50:46Z","timestamp":1419868246000},"page":"444-447","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":6,"title":["Preparation of Monoclonal Antibodies Against\n                    <i>Mycobacterium tuberculosis<\/i>\n                    TB10.4 Antigen"],"prefix":"10.1177","volume":"33","author":[{"given":"Xiang","family":"Chen","sequence":"first","affiliation":[{"name":"Yangzhou University","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhen","family":"Ou","sequence":"additional","affiliation":[{"name":"Yangzhou University","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao-Li","family":"Xie","sequence":"additional","affiliation":[{"name":"Yangzhou University","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zheng-Zhong","family":"Xu","sequence":"additional","affiliation":[{"name":"Yangzhou University","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xin-An","family":"Jiao","sequence":"additional","affiliation":[{"name":"Yangzhou University","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2014,12,29]]},"reference":[{"key":"e_1_3_3_2_2","unstructured":"World Health Organization Global Tuberculosis Control: Epidemiology Strategy Financing WHO Report 2009. Publication WHO\/HTM\/TB\/2009.411 Geneva Switzerland."},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/31159"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1130426100"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/35059006"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.248732"},{"key":"e_1_3_3_7_2","first-page":"1707","article-title":"Take five-Type VII secretion systems of Mycobacteria","volume":"18","author":"Houben EN","year":"2013","unstructured":"Houben EN, , Korotkov KV, , and Bitter W: Take five-Type VII secretion systems of Mycobacteria. Biochim Biophys Acta, 2013; 18:1707\u20131716.","journal-title":"Biochim Biophys Acta"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00284-013-0507-2"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.2.791-795.2000"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/icb.2009.14"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(08)61036-3"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01660-05"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.63.5.1710-1717.1995"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.174.10.6332"},{"key":"e_1_3_3_15_2","first-page":"391","volume-title":"Handbook of Laboratory Animal Science","author":"Hendriksen C","year":"2003","unstructured":"Hendriksen C, , and Hau J: Production of polyclonal and monoclonal antibodies. In: Handbook of Laboratory Animal Science, 2nd ed. CRC Press, Boca Raton, FL, 2003. pp. 391\u2013411.","edition":"2"},{"key":"e_1_3_3_16_2","unstructured":"Sambrook J and Russell DW: Molecular Cloning: A Laboratory Manual 3rd ed. Cold Spring Harbor Laboratory Press Cold Spring Harbor NY 2001."},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00004-07"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.10.5446-5453.2002"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0900589106"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00756-09"}],"container-title":["Monoclonal Antibodies in Immunodiagnosis &amp; Immunotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1089\/mab.2014.0039","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/mab.2014.0039","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/mab.2014.0039","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T20:17:03Z","timestamp":1777666623000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1089\/mab.2014.0039"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,12,1]]},"references-count":19,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2014,12,1]]}},"alternative-id":["10.1089\/mab.2014.0039"],"URL":"https:\/\/doi.org\/10.1089\/mab.2014.0039","relation":{},"ISSN":["2167-9436","2167-9436"],"issn-type":[{"value":"2167-9436","type":"print"},{"value":"2167-9436","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,12,1]]}}}