{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,7]],"date-time":"2026-05-07T01:51:15Z","timestamp":1778118675721,"version":"3.51.4"},"reference-count":34,"publisher":"SAGE Publications","issue":"2","license":[{"start":{"date-parts":[[2015,4,1]],"date-time":"2015-04-01T00:00:00Z","timestamp":1427846400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Monoclonal Antibodies in Immunodiagnosis &amp; Immunotherapy"],"published-print":{"date-parts":[[2015,4]]},"abstract":"<jats:p>\n                    <jats:italic toggle=\"yes\">Haemophilus ducreyi<\/jats:italic>\n                    is the causative agent of the sexually transmitted genital ulcer disease chancroid. Strains of\n                    <jats:italic toggle=\"yes\">H. ducreyi<\/jats:italic>\n                    are grouped in two classes (I and II) based on genotypic and phenotypic differences, including those found in DsrA, an outer membrane protein belonging to the family of multifunctional trimeric autotransporter adhesins. DsrA is a key serum resistance factor of\n                    <jats:italic toggle=\"yes\">H. ducreyi<\/jats:italic>\n                    that prevents binding of natural IgM at the bacterial surface and functions as an adhesin to fibronectin, fibrinogen, vitronectin, and human keratinocytes. Monoclonal antibodies (MAbs) were developed to recombinant DsrA (DsrA\n                    <jats:sub>I<\/jats:sub>\n                    ) from prototypical class I strain 35000HP to define targets for vaccine and\/or therapeutics. Two anti-DsrA\n                    <jats:sub>I<\/jats:sub>\n                    MAbs bound monomers and multimers of DsrA from genital and non-genital\/cutaneous\n                    <jats:italic toggle=\"yes\">H. ducreyi<\/jats:italic>\n                    strains in a Western blot and reacted to the surface of the genital strains; however, these MAbs did not recognize denatured or native DsrA from class II strains. In a modified extracellular matrix protein binding assay using viable\n                    <jats:italic toggle=\"yes\">H. ducreyi<\/jats:italic>\n                    , one of the MAbs partially inhibited binding of fibronectin, fibrinogen, and vitronectin to class I\n                    <jats:italic toggle=\"yes\">H. ducreyi<\/jats:italic>\n                    strain 35000HP, suggesting a role for anti-DsrA antibodies in preventing binding of\n                    <jats:italic toggle=\"yes\">H. ducreyi<\/jats:italic>\n                    to extracellular matrix proteins. Standard ELISA and surface plasmon resonance using a peptide library representing full-length, mature DsrA\n                    <jats:sub>I<\/jats:sub>\n                    revealed the smallest nominal epitope bound by one of the MAbs to be MEQNTHNINKLS. Taken together, our findings suggest that this epitope is a potential target for an\n                    <jats:italic toggle=\"yes\">H. ducreyi<\/jats:italic>\n                    vaccine.\n                  <\/jats:p>","DOI":"10.1089\/mab.2014.0054","type":"journal-article","created":{"date-parts":[[2015,4,21]],"date-time":"2015-04-21T15:33:07Z","timestamp":1429630387000},"page":"73-82","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":9,"title":["Defining Potential Vaccine Targets of\n                    <i>Haemophilus ducreyi<\/i>\n                    Trimeric Autotransporter Adhesin DsrA"],"prefix":"10.1177","volume":"34","author":[{"given":"William G.","family":"Fusco","sequence":"first","affiliation":[{"name":"University of North Carolina at Chapel Hill"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Neelima R.","family":"Choudhary","sequence":"additional","affiliation":[{"name":"University of North Carolina at Chapel Hill"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shelley M.","family":"Stewart","sequence":"additional","affiliation":[{"name":"Duke University Medical Center"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. 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