{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T19:18:37Z","timestamp":1777922317227,"version":"3.51.4"},"reference-count":30,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2015,6,1]],"date-time":"2015-06-01T00:00:00Z","timestamp":1433116800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Monoclonal Antibodies in Immunodiagnosis &amp; Immunotherapy"],"published-print":{"date-parts":[[2015,6]]},"abstract":"<jats:p>\n                    Folate receptors \u03b1 (FR\u03b1) and \u03b2 (FR\u03b2) are two isoforms of the cell surface glycoprotein that binds folate. The expression of FR\u03b1 is rare in normal cells and elevated in cancer cells. Thus, FR\u03b1-based tumor-targeted therapy has been a focus area of laboratory research and clinical trials. Recently, it was shown that a significant fraction of tumor-associated macrophages expresses FR\u03b2 and that these cells can enhance tumor growth. Although FR\u03b1 and FR\u03b2 share 70% identity in their deduced amino acid sequence, a monoclonal antibody (MAb) reactive with both receptors has not been developed. A MAb that can target both FR\u03b1-expressing cancer cells and FR\u03b2-expressing tumor-associated macrophages may provide a more potent therapeutic tool for cancer than individual anti-FR\u03b1 or anti-FR\u03b2 MAbs. In this study, we developed a MAb that recognizes both FR\u03b1 and FR\u03b2 (anti-FR\u03b1\u03b2). The anti-FR\u03b1\u03b2 specifically stained trophoblasts and macrophages from human placenta, synovial macrophages from rheumatoid arthritis patient, liver macrophages from cynomolgus monkey and common marmoset, and cancer cells and tumor-associated macrophages from ovary and lung carcinomas. Surface plasmon resonance showed that the anti-FR\u03b1\u03b2 bound to soluble forms of the FR\u03b1 and FR\u03b2 proteins with high affinity (\n                    <jats:italic toggle=\"yes\">K<\/jats:italic>\n                    <jats:sub>D<\/jats:sub>\n                    =6.26\u00d710\n                    <jats:sup>\u22129<\/jats:sup>\n                    \u2009M and 4.33\u00d710\n                    <jats:sup>\u22129<\/jats:sup>\n                    \u2009M, respectively).\n                    <jats:italic toggle=\"yes\">In vitro<\/jats:italic>\n                    functional analysis of the anti-FR\u03b1\u03b2 showed that this MAb mediates complement-dependent cytotoxicity, antibody-dependent cellular cytotoxicity, and antibody-dependent cellular phagocytosis of FR\u03b1-expressing and FR\u03b2-expressing cell lines. The anti-FR\u03b1\u03b2 MAb is a promising therapeutic candidate for cancers in which macrophages promote tumor progression.\n                  <\/jats:p>","DOI":"10.1089\/mab.2014.0072","type":"journal-article","created":{"date-parts":[[2015,6,19]],"date-time":"2015-06-19T15:06:21Z","timestamp":1434726381000},"page":"181-190","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":9,"title":["Production of a High-affinity Monoclonal Antibody Reactive with Folate Receptors Alpha and Beta"],"prefix":"10.1177","volume":"34","author":[{"given":"Taku","family":"Nagai","sequence":"first","affiliation":[{"name":"Kagoshima University","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuko","family":"Furusho","sequence":"additional","affiliation":[{"name":"Kagoshima 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