{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T19:19:10Z","timestamp":1777922350208,"version":"3.51.4"},"reference-count":31,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2017,2,1]],"date-time":"2017-02-01T00:00:00Z","timestamp":1485907200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Monoclonal Antibodies in Immunodiagnosis &amp; Immunotherapy"],"published-print":{"date-parts":[[2017,2]]},"abstract":"<jats:p>\n                    Parasitological methods are not helpful for the diagnosis of fascioliasis in acute and invasive periods of the disease. Detection of coproantigens seems to be a suitable alternative approach in the diagnosis of fascioliasis. The present study aimed to develop a reliable antigen detection system, using monoclonal antibodies raised against excretory-secretory (ES) antigen of\n                    <jats:italic toggle=\"yes\">Fasciola hepatica<\/jats:italic>\n                    , for the diagnosis of fascioliasis.\n                    <jats:italic toggle=\"yes\">Fasciola<\/jats:italic>\n                    adult worms were collected from the bile ducts of infected animals. Species of the fluke was determined by polymerase chain reaction\u2013restriction fragment length polymorphism (RFLP-PCR). ES antigen of\n                    <jats:italic toggle=\"yes\">F. hepatica<\/jats:italic>\n                    was prepared. For production of monoclonal antibodies, mice were immunized with ES antigens of\n                    <jats:italic toggle=\"yes\">F. hepatica<\/jats:italic>\n                    . Spleen cells from the immunized mice were fused with NS-1 myeloma cells, using polyethylene glycol. Hybridoma cells secreting specific antibody were expanded and cloned by limiting dilution. Moreover, polyclonal antibody was produced against\n                    <jats:italic toggle=\"yes\">F. hepatica<\/jats:italic>\n                    ES antigen in rabbits. A capture enzyme-linked immunosorbent assay (ELISA) system, using produced monoclonal antibody, was designed and stool samples of infected animals along with control samples were tested by the system. The capture ELISA detected the coproantigen in 27 of 30 (90%) parasitologically confirmed fascioliasis cases, while 4 of 39 (10.25%) samples infected with other parasitic infections showed a positive reaction in this system. No positive reactivity was found with healthy control samples. Accordingly, sensitivity of 90% and specificity of 94.2% were obtained for the capture ELISA system. The results were compared with those obtained with commercial BIO-X ELISA, and a very good (kappa\u2009=\u20090.9) agreement was found between the commercial kit and the developed capture ELISA. Findings of this study showed that the produced monoclonal antibody has appropriate performance for the detection of\n                    <jats:italic toggle=\"yes\">Fasciola<\/jats:italic>\n                    coproantigen in stool samples and can be appropriately used for the diagnosis of fascioliasis.\n                  <\/jats:p>","DOI":"10.1089\/mab.2016.0034","type":"journal-article","created":{"date-parts":[[2017,2,10]],"date-time":"2017-02-10T11:22:32Z","timestamp":1486725752000},"page":"8-14","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":7,"title":["Production of Monoclonal Antibody Against Excretory-Secretory Antigen of\n                    <i>Fasciola hepatica<\/i>\n                    and Evaluation of Its Efficacy in the Diagnosis of Fascioliasis"],"prefix":"10.1177","volume":"36","author":[{"given":"Samaneh","family":"Abdolahi Khabisi","sequence":"first","affiliation":[{"name":"Shiraz University of Medical Sciences","place":["Iran"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bahador","family":"Sarkari","sequence":"additional","affiliation":[{"name":"Shiraz University of Medical Sciences","place":["Iran"]},{"name":"Shiraz University of Medical Sciences","place":["Iran"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abdolali","family":"Moshfe","sequence":"additional","affiliation":[{"name":"Yasuj University of Medical Sciences","place":["Iran"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sedigheh","family":"Jalali","sequence":"additional","affiliation":[{"name":"Shiraz University of Medical Sciences","place":["Iran"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2017,2]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-308X(09)69002-3"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2005.07.010"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2005.07.010"},{"key":"e_1_3_3_5_2","first-page":"15","article-title":"Seroprevalence of human fasciolosis in a new-emerging focus of fasciolosis in Yasuj district, southwest of Iran","volume":"7","author":"Sarkari B","year":"2012","unstructured":"Sarkari B, , Ghobakhloo N, , Moshfea A, , and Eilami O: Seroprevalence of human fasciolosis in a new-emerging focus of fasciolosis in Yasuj district, southwest of Iran. 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