{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T19:19:20Z","timestamp":1777922360140,"version":"3.51.4"},"reference-count":15,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2017,7,13]],"date-time":"2017-07-13T00:00:00Z","timestamp":1499904000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Monoclonal Antibodies in Immunodiagnosis &amp; Immunotherapy"],"published-print":{"date-parts":[[2017,8,1]]},"abstract":"<jats:p>\n                    More recently, alternative splicing of specific genes are investigated for their therapeutic potential. In particular, we reported the existence of\n                    <jats:italic toggle=\"yes\">BCR-ABL<\/jats:italic>\n                    alternative splicing isoforms, in about 80% of Philadelphia-positive patients, which lead to the expression of aberrant proteins. These fusion proteins are characterized by an orphan initial and correct Bcr portion attached to a 112 amino acid sequence, arising from the impairment in the reading frame (reading of\n                    <jats:italic toggle=\"yes\">ABL<\/jats:italic>\n                    exon 4 and 5). We demonstrated that these Abl-out-of-frame (OOF) isoforms could have an immunological role with therapeutic implications. The aim of this study was to characterize a new monoclonal antibody (MAb) specific for Abl-OOF protein portion, for diagnostic use, to detect this biomarker in Philadelphia chromosome-positive chronic myelogenous leukemia (CML) patients and to generate novel approaches in the immunotherapy. 5F11G11 MAb recognizes the OOF protein portion of the native full-length Bcr\/Abl-OOF protein expressed in cells transiently transfected, as demonstrated by immunoprecipitation and immunofluorescence. In addition, we demonstrate the MAb's ability to recognize the alternative hybrid Bcr\/Abl fusion protein expressed in leukemic cells from CML patients, to support the possible use of 5F11G11 MAb as a diagnostic tool to select patients with Philadelphia chromosome-positive CML that could be eligible for an immunotherapeutic approach with this new antigen.\n                  <\/jats:p>","DOI":"10.1089\/mab.2016.0054","type":"journal-article","created":{"date-parts":[[2017,7,13]],"date-time":"2017-07-13T15:27:30Z","timestamp":1499959650000},"page":"149-156","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Specific Monoclonal Antibody Against Bcr\/Abl Out-of-Frame Alternative Proteins as Diagnostic Tool in Chronic Myelogenous Leukemia Patients"],"prefix":"10.1177","volume":"36","author":[{"given":"Claudia","family":"Casnici","sequence":"first","affiliation":[{"name":"Ferdinando Santarelli Foundation","place":["Italy"]},{"name":"University of Milan","place":["Italy"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katia","family":"Crotta","sequence":"additional","affiliation":[{"name":"University of Milan","place":["Italy"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gisella","family":"Volpe","sequence":"additional","affiliation":[{"name":"University of Turin","place":["Italy"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cristina","family":"Panuzzo","sequence":"additional","affiliation":[{"name":"University of Turin","place":["Italy"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Donatella","family":"Lattuada","sequence":"additional","affiliation":[{"name":"University of Milan","place":["Italy"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giulia","family":"Mesiano","sequence":"additional","affiliation":[{"name":"Candiolo Cancer Institute","place":["Italy"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giuseppe","family":"Saglio","sequence":"additional","affiliation":[{"name":"University of Turin","place":["Italy"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ornella","family":"Marelli","sequence":"additional","affiliation":[{"name":"University of Milan","place":["Italy"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2017,7,13]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-06-3737"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2008.10.032"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1089\/hyb.2010.0122"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1097\/CJI.0b013e3182562d37"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2407-6-262"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.18632\/oncoscience.260"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-60761-783-9_6"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(87)90021-2"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2013-05-501569"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2012.104"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2012.305"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2013-10-532341"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2008.06.003"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2013.00496"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2008.273"}],"container-title":["Monoclonal Antibodies in Immunodiagnosis &amp; 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