{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T19:19:46Z","timestamp":1777922386530,"version":"3.51.4"},"reference-count":40,"publisher":"SAGE Publications","issue":"6","license":[{"start":{"date-parts":[[2017,11,1]],"date-time":"2017-11-01T00:00:00Z","timestamp":1509494400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Monoclonal Antibodies in Immunodiagnosis &amp; Immunotherapy"],"published-print":{"date-parts":[[2017,12,1]]},"abstract":"<jats:p>Oral squamous cell carcinoma is an aggressive tumor in cats; however, molecular-targeted therapies against this tumor, including antibody therapy, have not been developed. Sensitive and specific monoclonal antibodies (mAbs) against highly expressed membrane proteins are needed to develop antibody therapies. Podoplanin, a type I transmembrane glycoprotein, is expressed in many human malignant tumors, including brain tumor, esophageal cancer, lung cancer, mesothelioma, and oral cancer. Podoplanin binds to C-type lectin-like receptor-2 (CLEC-2) and activates platelet aggregation, which is involved in cancer metastasis. Until now, we have established several mAbs against podoplanin in humans, mice, rats, rabbits, dogs, cattle, and cats. We have reported podoplanin expression in canine melanoma and squamous cell carcinomas using an anti-dog podoplanin mAb PMab-38. In this study, we investigated podoplanin expression in 40 feline squamous cell carcinomas (14 cases of mouth floor, 13 of skin, 9 of ear, and 4 of tongue) by immunohistochemical analysis using an anti-cat podoplanin mAb PMab-52, which we recently developed by cell-based immunization and screening (CBIS) method. Of the total 40 cases, 38 (95%) showed positive staining for PMab-52. In particular, 12 cases (30%) showed a strong membrane-staining pattern of squamous cell carcinoma cells. PMab-52 can be useful for antibody therapy against feline podoplanin-expressing squamous cell carcinomas.<\/jats:p>","DOI":"10.1089\/mab.2017.0046","type":"journal-article","created":{"date-parts":[[2017,11,1]],"date-time":"2017-11-01T11:01:05Z","timestamp":1509534065000},"page":"243-250","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":10,"title":["Expression of Cat Podoplanin in Feline Squamous Cell Carcinomas"],"prefix":"10.1177","volume":"36","author":[{"given":"Shunsuke","family":"Itai","sequence":"first","affiliation":[{"name":"Tohoku University Graduate School of Medicine","place":["Japan"]},{"name":"Tokyo Medical and Dental University","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shinji","family":"Yamada","sequence":"additional","affiliation":[{"name":"Tohoku University Graduate School of Medicine","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mika K.","family":"Kaneko","sequence":"additional","affiliation":[{"name":"Tohoku University Graduate School of Medicine","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroyuki","family":"Harada","sequence":"additional","affiliation":[{"name":"Tokyo Medical and Dental University","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yumiko","family":"Kagawa","sequence":"additional","affiliation":[{"name":"North Lab, Hokkaido, Sapporo, Hokkaido, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Satoru","family":"Konnai","sequence":"additional","affiliation":[{"name":"Hokkaido University","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yukinari","family":"Kato","sequence":"additional","affiliation":[{"name":"Tohoku University Graduate School of Medicine","place":["Japan"]},{"name":"Tohoku University","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2017,11,1]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1601-0825.1996.tb00216.x"},{"key":"e_1_3_3_3_2","first-page":"1141","article-title":"Podoplanin, novel 43-kd membrane protein of glomerular epithelial cells, is down-regulated in puromycin nephrosis","volume":"151","author":"Breiteneder-Geleff S","year":"1997","unstructured":"Breiteneder-Geleff S, , Matsui K, , Soleiman A, , Meraner P, , Poczewski H, , Kalt R, , Schaffner G, , and Kerjaschki D: Podoplanin, novel 43-kd membrane protein of glomerular epithelial cells, is down-regulated in puromycin nephrosis. 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