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PDPN expression is strongly associated with cancer progression and poor prognosis. Furthermore, PDPN binds to C-type lectin-like receptor 2 (CLEC-2) on platelets, followed by PDPN-mediated platelet aggregation to facilitate tumor metastasis. We have previously reported a novel anti-cat PDPN (cPDPN) monoclonal antibody (mAb), PMab-52, which specifically detects cPDPN using flow cytometry analysis and successfully identifies cPDPN in feline squamous cell carcinomas. However, the specific binding epitope of cPDPN for PMab-52 remains unelucidated. In this study, a series of deletion or point mutants of cPDPN were utilized for investigating the binding epitopes of PMab-52 using flow cytometry and Western blotting. The findings of this study revealed that the critical epitopes of platelet aggregation-stimulating domain 4 (PLAG4) of cPDPN are responsible for the binding of PMab-52 to cPDPN.<\/jats:p>","DOI":"10.1089\/mab.2017.0067","type":"journal-article","created":{"date-parts":[[2018,2,2]],"date-time":"2018-02-02T15:06:16Z","timestamp":1517583976000},"page":"95-99","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":10,"title":["Epitope Mapping of Monoclonal Antibody PMab-52 Against Cat Podoplanin"],"prefix":"10.1177","volume":"37","author":[{"given":"Yao-Wen","family":"Chang","sequence":"first","affiliation":[{"name":"Tohoku University Graduate School of Medicine","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mika K.","family":"Kaneko","sequence":"additional","affiliation":[{"name":"Tohoku University Graduate School of Medicine","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shinji","family":"Yamada","sequence":"additional","affiliation":[{"name":"Tohoku University Graduate School of Medicine","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yukinari","family":"Kato","sequence":"additional","affiliation":[{"name":"Tohoku University Graduate School of Medicine","place":["Japan"]},{"name":"Tohoku University","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2018,2,2]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M309935200"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.20656"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2006.04.023"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/ndt\/14.suppl_1.9"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1827.2005.01791.x"},{"key":"e_1_3_3_7_2","first-page":"1141","article-title":"Podoplanin, novel 43-kd membrane protein of glomerular epithelial cells, is down-regulated in puromycin nephrosis","volume":"151","author":"Breiteneder-Geleff S","year":"1997","unstructured":"Breiteneder-Geleff S, , Matsui K, , Soleiman A, , Meraner P, , Poczewski H, , Kalt R, , Schaffner G, , and Kerjaschki D: Podoplanin, novel 43-kd membrane protein of glomerular epithelial cells, is down-regulated in puromycin nephrosis. 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