{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T19:20:05Z","timestamp":1777922405056,"version":"3.51.4"},"reference-count":24,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[2018,10,1]],"date-time":"2018-10-01T00:00:00Z","timestamp":1538352000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Monoclonal Antibodies in Immunodiagnosis &amp; Immunotherapy"],"published-print":{"date-parts":[[2018,10,1]]},"abstract":"<jats:p>\n                    <jats:italic toggle=\"yes\">Staphylococcus aureus<\/jats:italic>\n                    secretes a family of exoproteins structurally homologous to bacterial superantigens, such as toxic shock syndrome toxin-1 (TSST-1), and those exoproteins are thus called staphylococcal superantigen-like proteins (SSLs). Recent studies have revealed that SSLs play roles in evasion of the host defense by disturbing host immune responses. We previously reported that staphylococcal superantigen-like protein 5 (SSL5; a member of the SSL family) inhibited matrix metalloproteinase-9 (MMP-9), which is crucial for leukocyte recruitment to sites of infection. In this study, we established a mouse hybridoma clone (30G5C) producing a monoclonal antibody specific for SSL5. In immunoblotting analysis using recombinant His-tagged SSL1 to SSL14 (His-SSLs), the antibody was found to specifically recognize SSL5 without crossreactivity with other His-SSLs. The antibody bound to the\n                    <jats:italic toggle=\"yes\">C<\/jats:italic>\n                    -terminal region of SSL5 (\u03b2-grasp domain), but did not interfere with the binding of SSL5 to MMP-9, suggesting that this antibody is useful for identification of SSL5-producing\n                    <jats:italic toggle=\"yes\">S. aureus<\/jats:italic>\n                    and screening for inhibitors of the SSL5\/MMP-9 complex formation.\n                  <\/jats:p>","DOI":"10.1089\/mab.2018.0016","type":"journal-article","created":{"date-parts":[[2018,10,26]],"date-time":"2018-10-26T12:58:54Z","timestamp":1540558734000},"page":"212-217","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":3,"title":["Generation of a Monoclonal Antibody Against Staphylococcal Superantigen-Like Protein 5 (SSL5) That Discriminates SSL5 from Other SSL Proteins"],"prefix":"10.1177","volume":"37","author":[{"given":"Teruaki","family":"Oku","sequence":"first","affiliation":[{"name":"Hoshi University School of Pharmacy and Pharmaceutical Sciences","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hikari","family":"Soma","sequence":"additional","affiliation":[{"name":"Hoshi University School of Pharmacy and Pharmaceutical Sciences","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chisato","family":"Kurisaka","sequence":"additional","affiliation":[{"name":"Hoshi University School of Pharmacy and Pharmaceutical Sciences","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tsutomu","family":"Tsuji","sequence":"additional","affiliation":[{"name":"Hoshi University School of Pharmacy and Pharmaceutical Sciences","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2018,10,24]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1289"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2007.06.005"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2013.06.012"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10529-017-2374-6"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2011.07720.x"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00399-12"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00109-012-0926-8"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.174.5.2926"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1593\/neo.81508"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2009.09.027"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0903311"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2006-06-015461"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00178-10"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/1348-0421.12573"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1538-7836.2009.03564.x"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0019190"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.8.4407-4415.2000"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.2008.00681.x"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2007.09.091"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112.349829"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj2850603"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20041020"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1089\/mab.2015.0053"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1111\/jth.12022"}],"container-title":["Monoclonal Antibodies in Immunodiagnosis &amp; 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