{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T19:20:37Z","timestamp":1777922437041,"version":"3.51.4"},"reference-count":12,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[2019,10,1]],"date-time":"2019-10-01T00:00:00Z","timestamp":1569888000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Monoclonal Antibodies in Immunodiagnosis &amp; Immunotherapy"],"published-print":{"date-parts":[[2019,10,1]]},"abstract":"<jats:p>\n                    Several members of enteroviruses (EVs) that belong to the EVs A and B species cause hand, foot, and mouth disease (HFMD) in infants and young children. The virus-specific protease 2A\n                    <jats:sup>pro<\/jats:sup>\n                    is conserved in all the EV species, thus developing a monoclonal antibody (mAb) against 2A\n                    <jats:sup>pro<\/jats:sup>\n                    may facilitate the identification from the HFMD-associated pathogens. In this study, we achieved a murine mAb, named 5A3, specifically toward EVA71 2A\n                    <jats:sup>pro<\/jats:sup>\n                    by using the traditional hybridoma technique. The mAb 5A3 recognizes 2A\n                    <jats:sup>pro<\/jats:sup>\n                    of both EVs A and B species, which was demonstrated by indirect fluorescent assay and Western blotting. Furthermore, a conserved N-terminal epitope on 2A\n                    <jats:sup>pro<\/jats:sup>\n                    recognized by mAb 5A3 was defined by using an overlapping peptide-based enzyme-linked immunosorbent assay (ELISA). Therefore, the unique mAb targeting conserved EVs 2A\n                    <jats:sup>pro<\/jats:sup>\n                    can be used as an important tool during both identifying the causative agent of HFMD and elucidating the pathological mechanism of HFMD.\n                  <\/jats:p>","DOI":"10.1089\/mab.2019.0026","type":"journal-article","created":{"date-parts":[[2019,10,11]],"date-time":"2019-10-11T16:48:00Z","timestamp":1570812480000},"page":"220-223","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":5,"title":["Monoclonal Antibody Targeting a Conserved N-Terminal Epitope on 2A\n                    <sup>pro<\/sup>\n                    of Enteroviruses"],"prefix":"10.1177","volume":"38","author":[{"given":"Kai","family":"Yi","sequence":"first","affiliation":[{"name":"Hubei Engineering Research Center of Viral Vector, Applied Biotechnology Research Center, Wuhan University of Bioengineering, Wuhan, China."},{"name":"College of Life Science and Technology, Wuhan University of Bioengineering, Wuhan, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lilan","family":"Xie","sequence":"additional","affiliation":[{"name":"Hubei Engineering Research Center of Viral Vector, Applied Biotechnology Research Center, Wuhan University of Bioengineering, Wuhan, China."},{"name":"College of Life Science and Technology, Wuhan University of Bioengineering, Wuhan, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yaoming","family":"Li","sequence":"additional","affiliation":[{"name":"Hubei Engineering Research Center of Viral Vector, Applied Biotechnology Research Center, Wuhan University of Bioengineering, Wuhan, China."},{"name":"College of Life Science and Technology, Wuhan University of Bioengineering, Wuhan, China."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2019,10,10]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00430-016-0465-y"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2014.2578"},{"key":"e_1_3_3_4_2","first-page":"1168","article-title":"[Etiology and clinical analysis of central nervous system infection caused by Coxsackievirus B5 in severe hand, foot and mouth disease in Qingdao City, 2013\u20132014]","volume":"52","author":"Liang RX","year":"2018","unstructured":"Liang RX, , Shi XY, , Zhang YH, , Su ZL, , Zhang F, , Chai Q, , Gong JL, , and Wang ZG: [Etiology and clinical analysis of central nervous system infection caused by Coxsackievirus B5 in severe hand, foot and mouth disease in Qingdao City, 2013\u20132014]. Zhonghua Yu Fang Yi Xue Za Zhi, 2018; 52:1168\u20131172.","journal-title":"Zhonghua Yu Fang Yi Xue Za Zhi"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2006.08.012"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02712-13"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003231"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2018.02.001"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1089\/hyb.2011.0083"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1089\/mab.2018.0021"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2010.04.015"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2012.06.025"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.03541-12"}],"container-title":["Monoclonal Antibodies in Immunodiagnosis &amp; Immunotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1089\/mab.2019.0026","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/mab.2019.0026","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/mab.2019.0026","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T20:20:44Z","timestamp":1777666844000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1089\/mab.2019.0026"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,10,1]]},"references-count":12,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2019,10,1]]}},"alternative-id":["10.1089\/mab.2019.0026"],"URL":"https:\/\/doi.org\/10.1089\/mab.2019.0026","relation":{},"ISSN":["2167-9436","2167-9436"],"issn-type":[{"value":"2167-9436","type":"print"},{"value":"2167-9436","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,10,1]]}}}