{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T01:11:35Z","timestamp":1790644295920,"version":"4.1.0"},"reference-count":20,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2020,8,1]],"date-time":"2020-08-01T00:00:00Z","timestamp":1596240000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Monoclonal Antibodies in Immunodiagnosis &amp; Immunotherapy"],"published-print":{"date-parts":[[2020,8]]},"abstract":"<jats:p>Understanding of the microenvironment of cancer plays a crucial role in cancer research. A tool is needed to evaluate the immune cells surrounding the cancer cells. This study establishes and evaluates a novel monoclonal antibody against canine CD8\u03b1 (cCD8\u03b1). The antibody was produced by immunization of rats with cCD8\u03b1-expressing cells. After establishment and selection of hybridoma cells, the clone F3\u2013B2 was established. The reactivity of F3\u2013B2 was confirmed using cCD8\u03b1-overexpressing murine cells. Flow cytometric analysis also demonstrated that F3\u2013B2 reacts with cCD8\u03b1 naturally expressed in canine peripheral blood mononuclear cells and a canine T cell lymphoma cell line. The specimens of lymphoid tissue showed immunohistochemical staining for F3\u2013B2. Moreover, we also found that F3\u2013B2 exhibited reactivity against feline CD8. Thus, this antibody provides a good research tool to analyze CD8-positive cytotoxic lymphocytes in canine and feline tumors.<\/jats:p>","DOI":"10.1089\/mab.2020.0002","type":"journal-article","created":{"date-parts":[[2020,6,17]],"date-time":"2020-06-17T11:49:18Z","timestamp":1592394558000},"page":"129-134","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":14,"title":["Establishment and Characterization of Monoclonal Antibody Against Canine CD8 Alpha"],"prefix":"10.1177","volume":"39","author":[{"given":"Osamu","family":"Sakai","sequence":"first","affiliation":[{"name":"Laboratory of Molecular Diagnostics and Therapeutics, Joint Graduate School of Veterinary Medicine, Yamaguchi University, Yamaguchi, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatsuhito","family":"Ii","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Pathology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuyuki","family":"Uchida","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Pathology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masaya","family":"Igase","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Diagnostics and Therapeutics, Joint Graduate School of Veterinary Medicine, Yamaguchi University, Yamaguchi, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takuya","family":"Mizuno","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Diagnostics and Therapeutics, Joint Graduate School of Veterinary Medicine, Yamaguchi University, Yamaguchi, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2020,8,21]]},"reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1186\/1746-6148-5-27"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/ilar.51.3.208"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejca.2003.11.031"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1004594107"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm.2012.01.006"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5829.2012.00326.x"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0103249"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-0039.1992.tb01963.x"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1590\/S0103-84782009005000025"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jvir.2019.10.015"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-165X.1995.tb00951.x"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1177\/030098589603300210"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.001561"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm.2018.02.007"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm.2011.04.009"},{"key":"e_1_3_4_17_2","first-page":"195","article-title":"Programmed cell death ligand 1 expression in canine cancer","volume":"30","author":"Shosu K","year":"2016","unstructured":"Shosu K, , Sakurai M, , Inoue K, , Nakagawa T, , Sakai H, , Morimoto M, , Okuda M, , Noguchi S, , and Mizuno T: Programmed cell death ligand 1 expression in canine cancer. 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