{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T19:20:50Z","timestamp":1777922450206,"version":"3.51.4"},"reference-count":18,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2020,8,1]],"date-time":"2020-08-01T00:00:00Z","timestamp":1596240000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Monoclonal Antibodies in Immunodiagnosis &amp; Immunotherapy"],"published-print":{"date-parts":[[2020,8]]},"abstract":"<jats:p>\n                    Sensitive and specific monoclonal antibodies (mAbs) are needed for detecting CD20. This antigen, one of several B lymphocyte antigens, is a target for every application used in the diagnosis of B cell lymphoma. Many anti-CD20 mAbs have been established, although applications of these antibodies are limited. This study aims to establish sensitive and specific anti-CD20 mAbs suitable for broad application, such as flow cytometry, Western blotting, and immunohistochemical analyses. Using the Cell-Based Immunization and Screening (CBIS) method, all procedures were performed by utilizing CD20-stable transfectants, and a clone, C\n                    <jats:sub>20<\/jats:sub>\n                    Mab-60 (IgG\n                    <jats:sub>2a<\/jats:sub>\n                    , kappa), was developed. In flow cytometry, C\n                    <jats:sub>20<\/jats:sub>\n                    Mab-60 detected overexpression of CD20 in LN229 cell and endogenous CD20 in BALL-1 (a human B cell leukemia cell line) but did not react with CD20-knockout BALL-1 (BINDS-24), indicating specificity for CD20. In Western blotting, C\n                    <jats:sub>20<\/jats:sub>\n                    Mab-60 detected CD20-overexpressing Chinese hamster ovary-K1, BALL-1, and Raji (a human Burkitt's lymphoma cell line) displaying both sensitivity and specificity. Furthermore, B cell but not T cell lymphomas were strongly stained with C\n                    <jats:sub>20<\/jats:sub>\n                    Mab-60 in immunohistochemical analyses. C\n                    <jats:sub>20<\/jats:sub>\n                    Mab-60, which was developed by CBIS method, is shown to be useful for the detection of cells expressing CD20 in lymphoma tissues by flow cytometry, Western blotting, and immunohistochemical analyses.\n                  <\/jats:p>","DOI":"10.1089\/mab.2020.0015","type":"journal-article","created":{"date-parts":[[2020,7,15]],"date-time":"2020-07-15T11:56:41Z","timestamp":1594814201000},"page":"112-116","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":29,"title":["Establishment of an Anti-CD20 Monoclonal Antibody (C\n                    <sub>20<\/sub>\n                    Mab-60) for Immunohistochemical Analyses"],"prefix":"10.1177","volume":"39","author":[{"given":"Yoshikazu","family":"Furusawa","sequence":"first","affiliation":[{"name":"Department of Antibody Drug Development, Tohoku University Graduate School of Medicine, Sendai, Japan."},{"name":"New Industry Creation Hatchery Center, Tohoku University, Sendai, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mika K.","family":"Kaneko","sequence":"additional","affiliation":[{"name":"Department of Antibody Drug Development, Tohoku University Graduate School of Medicine, Sendai, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yukinari","family":"Kato","sequence":"additional","affiliation":[{"name":"Department of Antibody Drug Development, Tohoku University Graduate School of Medicine, Sendai, Japan."},{"name":"New Industry Creation Hatchery Center, Tohoku University, Sendai, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2020,8,21]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.135.2.973"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2141.1997.72653.x"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1309\/3WG32YRAMQ7RB9D4"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M800784200"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M308802200"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1089\/mab.2017.0031"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrep.2018.03.007"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1089\/mab.2018.0004"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1089\/mab.2018.0038"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1089\/mab.2019.0003"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrep.2019.100644"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrep.2019.100631"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrep.2019.01.009"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1089\/mab.2019.0013"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrep.2019.100633"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1089\/mab.2018.0048"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V83.2.435.435"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.2165\/11201110-000000000-00000"}],"container-title":["Monoclonal Antibodies in Immunodiagnosis &amp; Immunotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1089\/mab.2020.0015","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/mab.2020.0015","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/mab.2020.0015","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T20:21:04Z","timestamp":1777666864000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/full\/10.1089\/mab.2020.0015"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,8]]},"references-count":18,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2020,8]]}},"alternative-id":["10.1089\/mab.2020.0015"],"URL":"https:\/\/doi.org\/10.1089\/mab.2020.0015","relation":{},"ISSN":["2167-9436","2167-9436"],"issn-type":[{"value":"2167-9436","type":"print"},{"value":"2167-9436","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,8]]}}}