{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T19:21:01Z","timestamp":1777922461399,"version":"3.51.4"},"reference-count":19,"publisher":"SAGE Publications","issue":"2","license":[{"start":{"date-parts":[[2021,4,1]],"date-time":"2021-04-01T00:00:00Z","timestamp":1617235200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Monoclonal Antibodies in Immunodiagnosis &amp; Immunotherapy"],"published-print":{"date-parts":[[2021,4]]},"abstract":"<jats:p>\n                    The DNA fragment encoding predicted main antigenic region, aa 14\u2013245 on N protein of Rift Valley virus (RVFV) was cloned into the vector pET-28a (+) and p3xFLAG-CMV-10. The recombinant pET-28a-N1 protein was expressed in\n                    <jats:italic toggle=\"yes\">Escherichia coli<\/jats:italic>\n                    BL21 (DE3) with 1\u2009mM isopropyl-\n                    <jats:italic toggle=\"yes\">b<\/jats:italic>\n                    -thio-galactopyranoside at 37\u00b0C for 5 hours, and purified by protein purifier. Three monoclonal antibodies (mAbs) named 3A5, 3A6, and 3A7 against N protein were obtained by fusing mouse myeloma cell line SP2\/0 with spleen lymphocytes from pET-28a-N1 protein-immunized mice. Finally, the mAbs were characterized by enzyme-linked immunosorbent assays, indirect immunofluorescent assays, and Western blot. The results show that all the mAbs possess high specificity and react with both prokaryotic and eukaryotic N protein, which could provide important materials for the research on the function of N protein and the diagnostic methods of RVFV.\n                  <\/jats:p>","DOI":"10.1089\/mab.2021.0002","type":"journal-article","created":{"date-parts":[[2021,4,26]],"date-time":"2021-04-26T12:40:10Z","timestamp":1619440810000},"page":"60-64","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":2,"title":["Production and Characterization of Monoclonal Antibodies Against N Protein of Rift Valley Fever Virus"],"prefix":"10.1177","volume":"40","author":[{"given":"Jing","family":"Rao","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, P.R. China."},{"name":"State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, P.R. China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"Ye","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, P.R. China."},{"name":"State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, P.R. China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shengbo","family":"Cao","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, P.R. China."},{"name":"State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, P.R. China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xueqin","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, P.R. 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