{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T19:21:25Z","timestamp":1777922485587,"version":"3.51.4"},"reference-count":11,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2022,2,1]],"date-time":"2022-02-01T00:00:00Z","timestamp":1643673600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Monoclonal Antibodies in Immunodiagnosis &amp; Immunotherapy"],"published-print":{"date-parts":[[2022,2]]},"abstract":"<jats:p>Rabies is a highly neurotropic disease caused by rabies lyssavirus (RABV). Human rabies vaccines exist for pre- and postexposure prophylaxis; however, after clinical symptoms appear, the disease has an \u223c100% mortality rate with no effective treatments available. In our previous study, mouse neuroblastoma cells transfected with a plasmid coding one clone of a single-chain variable fragment (scFv), scFv-P19, against RABV phosphoprotein (RABV-P) derived from an scFv phage-display library, before infection, exhibited reduced viral propagation after infection with the RABV-fixed strain, CVS11. In this study, we conducted epitope mapping of scFv-P19 through indirect fluorescent assay and Western blotting analysis against full-length and N- or C-terminal truncated RABV-P. Our results suggest that scFv-P19 targets a portion containing amino acids 47\u201352 at the N-terminus, which partially overlaps with the N-terminal nuclear export sequences. This provides insights into the underlying mechanism associated with inhibition of RABV by scFv-P19, while allowing for the design of additional scFv-based therapeutic studies for RABV by integrating appropriate delivery and application systems. Furthermore, the results of this study suggest that scFv-P19 may serve as an effective tool for investigating nuclear trafficking of RABV-P to explore the roles of RABV-P isoforms in rabies pathogenesis.<\/jats:p>","DOI":"10.1089\/mab.2021.0020","type":"journal-article","created":{"date-parts":[[2022,2,28]],"date-time":"2022-02-28T11:58:10Z","timestamp":1646049490000},"page":"27-31","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Epitope Mapping of A Viral Propagation-Inhibiting Single-Chain Variable Fragment Against Rabies Lyssavirus Phosphoprotein"],"prefix":"10.1177","volume":"41","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7528-2805","authenticated-orcid":false,"given":"Yoshihiro","family":"Kaku","sequence":"first","affiliation":[{"name":"Department of Veterinary Science, National Institute of Infectious Diseases, Shinjuku-ku, Tokyo, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akiko","family":"Okutani","sequence":"additional","affiliation":[{"name":"Department of Veterinary Science, National Institute of Infectious Diseases, Shinjuku-ku, Tokyo, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akira","family":"Noguchi","sequence":"additional","affiliation":[{"name":"Department of Veterinary Science, National Institute of Infectious Diseases, Shinjuku-ku, Tokyo, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Satoshi","family":"Inoue","sequence":"additional","affiliation":[{"name":"Department of Veterinary Science, National Institute of Infectious Diseases, Shinjuku-ku, Tokyo, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ken","family":"Maeda","sequence":"additional","affiliation":[{"name":"Department of Veterinary Science, National Institute of Infectious Diseases, Shinjuku-ku, Tokyo, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shigeru","family":"Morikawa","sequence":"additional","affiliation":[{"name":"Department of Veterinary Science, National Institute of Infectious Diseases, Shinjuku-ku, Tokyo, Japan."},{"name":"Faculty of Veterinary Medicine, Okayama University of Science, Imabari, Ehime, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2022,2,28]]},"reference":[{"key":"e_1_3_5_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.antiviral.2011.04.016"},{"key":"e_1_3_5_3_2","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/980250"},{"key":"e_1_3_5_4_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-75-11-2889"},{"key":"e_1_3_5_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.72.3.1925-1930.1998"},{"key":"e_1_3_5_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01930-06"},{"key":"e_1_3_5_7_2","doi-asserted-by":"publisher","DOI":"10.7883\/yoken.JJID.2005.115"},{"key":"e_1_3_5_8_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112.374694"},{"key":"e_1_3_5_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00809-16"},{"key":"e_1_3_5_10_2","doi-asserted-by":"publisher","DOI":"10.1007\/s40259-020-00419-w"},{"key":"e_1_3_5_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2015.11.018"},{"key":"e_1_3_5_12_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12964-020-00644-4"}],"container-title":["Monoclonal Antibodies in Immunodiagnosis &amp; Immunotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1089\/mab.2021.0020","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/mab.2021.0020","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/mab.2021.0020","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T20:21:28Z","timestamp":1777666888000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/full\/10.1089\/mab.2021.0020"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,2]]},"references-count":11,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2022,2]]}},"alternative-id":["10.1089\/mab.2021.0020"],"URL":"https:\/\/doi.org\/10.1089\/mab.2021.0020","relation":{},"ISSN":["2167-9436","2167-9436"],"issn-type":[{"value":"2167-9436","type":"print"},{"value":"2167-9436","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,2]]}}}