{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T19:21:29Z","timestamp":1777922489975,"version":"3.51.4"},"reference-count":20,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2022,2,1]],"date-time":"2022-02-01T00:00:00Z","timestamp":1643673600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Monoclonal Antibodies in Immunodiagnosis &amp; Immunotherapy"],"published-print":{"date-parts":[[2022,2]]},"abstract":"<jats:p>CD10 is a glycosylated transmembrane protein and is known as a membrane endopeptidase. CD10 is expressed on predifferentiated lymphocyte progenitor, epithelial, stromal, and tumor cells. Antibodies against CD10 are used for the diagnosis of follicular lymphoma. Anti-human CD10 monoclonal antibody (clone MME\/1870) can be used for Western blotting and immunohistochemical analyses. This study examined the critical epitope of MME\/1870 using enzyme-linked immunosorbent assay (ELISA) with synthesized peptides. First, we performed ELISA with deletion mutants, and MME\/1870 reacted to the 501\u2013520 amino acid sequence of CD10. Next, we analyzed the reaction to 20 point mutants, and MME\/1870 did not recognize the alanine-substituted peptides of Y507A, I511A, I512A, and L515A. These results indicate that the binding epitope of MME\/1870 includes Tyr507, Ile511, Ile512, and Leu515 of CD10.<\/jats:p>","DOI":"10.1089\/mab.2021.0046","type":"journal-article","created":{"date-parts":[[2022,2,28]],"date-time":"2022-02-28T11:58:10Z","timestamp":1646049490000},"page":"15-19","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":2,"title":["Epitope Mapping of an Anti-CD10 Monoclonal Antibody (MME\/1870) Using Enzyme-Linked Immunosorbent Assay"],"prefix":"10.1177","volume":"41","author":[{"given":"Hiroki","family":"Kawabata","sequence":"first","affiliation":[{"name":"Department of Antibody Drug Development, Tohoku University Graduate School of Medicine, Sendai, Miyagi, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroyuki","family":"Suzuki","sequence":"additional","affiliation":[{"name":"Department of Molecular Pharmacology, Tohoku University Graduate School of Medicine, Sendai, Miyagi, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junko","family":"Takei","sequence":"additional","affiliation":[{"name":"Department of Antibody Drug Development, Tohoku University Graduate School of Medicine, Sendai, Miyagi, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mika K.","family":"Kaneko","sequence":"additional","affiliation":[{"name":"Department of Antibody Drug Development, Tohoku University Graduate School of Medicine, Sendai, Miyagi, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5385-8201","authenticated-orcid":false,"given":"Yukinari","family":"Kato","sequence":"additional","affiliation":[{"name":"Department of Antibody Drug Development, Tohoku University Graduate School of Medicine, Sendai, Miyagi, Japan."},{"name":"Department of Molecular Pharmacology, Tohoku University Graduate School of Medicine, Sendai, Miyagi, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2022,2,28]]},"reference":[{"key":"e_1_3_3_2_2","first-page":"423","article-title":"Acute lymphocytic leukemia-associated cell membrane antigen","volume":"61","author":"Billing R","year":"1978","unstructured":"Billing R, , Minowada J, , Cline M, , Clark B, , and Lee K: Acute lymphocytic leukemia-associated cell membrane antigen. 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