{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T19:21:28Z","timestamp":1777922488927,"version":"3.51.4"},"reference-count":37,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2023,2,1]],"date-time":"2023-02-01T00:00:00Z","timestamp":1675209600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Monoclonal Antibodies in Immunodiagnosis &amp; Immunotherapy"],"published-print":{"date-parts":[[2023,2]]},"abstract":"<jats:p>\n                    An anti-mouse CXC chemokine receptor 6 (mCXCR6) monoclonal antibody (mAb), Cx\n                    <jats:sub>6<\/jats:sub>\n                    Mab-1, was developed recently. Cx\n                    <jats:sub>6<\/jats:sub>\n                    Mab-1 is applicable for flow cytometry, Western blotting, and enzyme-linked immunosorbent assay. The purpose of this study is to determine the binding epitope of Cx\n                    <jats:sub>6<\/jats:sub>\n                    Mab-1 using 2\u2009\u00d7\u2009alanine mutated mCXCR6. Analysis of flow cytometry revealed that Cx\n                    <jats:sub>6<\/jats:sub>\n                    Mab-1 did not recognize S8A-A9G, L10A-Y11A, D12A-G13A, and H14A-Y15A mutants of mCXCR6. The results clearly indicate that the binding epitope of Cx\n                    <jats:sub>6<\/jats:sub>\n                    Mab-1 includes Ser8, Ala9, Leu10, Tyr11, Asp12, Gly13, His14, and Tyr15 of mCXCR6. The successful determination of the Cx\n                    <jats:sub>6<\/jats:sub>\n                    Mab-1 epitope might contribute to the pathophysiological investigation of mCXCR6.\n                  <\/jats:p>","DOI":"10.1089\/mab.2022.0029","type":"journal-article","created":{"date-parts":[[2022,11,16]],"date-time":"2022-11-16T10:11:35Z","timestamp":1668593495000},"page":"22-26","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":3,"title":["Epitope Mapping Using the Cell-Based 2\u2009\u00d7\u2009Alanine Substitution Method About the Anti-mouse CXCR6 Monoclonal Antibody, Cx\n                    <sub>6<\/sub>\n                    Mab-1"],"prefix":"10.1177","volume":"42","author":[{"given":"Yu","family":"Isoda","sequence":"first","affiliation":[{"name":"Department of Antibody Drug Development, Tohoku University Graduate School of Medicine, Sendai, Miyagi, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomohiro","family":"Tanaka","sequence":"additional","affiliation":[{"name":"Department of Antibody Drug Development, Tohoku University Graduate School of Medicine, Sendai, Miyagi, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroyuki","family":"Suzuki","sequence":"additional","affiliation":[{"name":"Department of Molecular Pharmacology, Tohoku University Graduate School of Medicine, Sendai, Miyagi, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Teizo","family":"Asano","sequence":"additional","affiliation":[{"name":"Department of Antibody Drug Development, Tohoku University Graduate School of Medicine, Sendai, Miyagi, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7056-6021","authenticated-orcid":false,"given":"Takeo","family":"Yoshikawa","sequence":"additional","affiliation":[{"name":"Department of 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