{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T18:48:20Z","timestamp":1780598900592,"version":"3.54.1"},"reference-count":21,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[2023,10,1]],"date-time":"2023-10-01T00:00:00Z","timestamp":1696118400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Monoclonal Antibodies in Immunodiagnosis &amp; Immunotherapy"],"published-print":{"date-parts":[[2023,10]]},"abstract":"<jats:p>Neuropilin-2 (NRP2) is a cell surface receptor that plays key roles in lymphangiogenesis, but also in pathophysiological conditions such as cancer and inflammation. NRP2 targeting by efzofitimod, a novel immunomodulatory molecule, is currently being tested for the treatment of pulmonary sarcoidosis. To date, no anti-NRP2 antibodies are available for companion diagnostics. Here we describe the development and characterization of a novel NRP2 antibody. Using a variety of research techniques, that is, enzyme-linked immunoassay, Western blot, biolayer interferometry, and immunohistochemistry, we demonstrate that our antibody detects all major NRP2 isoforms and does not cross-react with NRP1. Using this antibody, we show high NRP2 expression in granulomas from sarcoidosis patient skin and lung biopsies. Our novel anti-NRP2 antibody could prove to be a useful clinical tool for sarcoidosis and other indications where NRP2 has been implicated.<\/jats:p>\n                  <jats:sec>\n                    <jats:title>Clinical Trial Registration:<\/jats:title>\n                    <jats:p>\n                      <jats:uri xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/clinicaltrials.gov\">clinicaltrials.gov<\/jats:uri>\n                      NCT05415137.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1089\/mab.2023.0007","type":"journal-article","created":{"date-parts":[[2023,10,30]],"date-time":"2023-10-30T12:28:20Z","timestamp":1698668900000},"page":"157-165","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":7,"title":["Development and Characterization of a Novel Neuropilin-2 Antibody for Immunohistochemical Staining of Cancer and Sarcoidosis Tissue Samples"],"prefix":"10.1177","volume":"42","author":[{"given":"Sarah","family":"F\u00f6rster","sequence":"first","affiliation":[{"name":"Institute of Pathology, University Hospital Bonn, Bonn, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yeeting E.","family":"Chong","sequence":"additional","affiliation":[{"name":"aTyr Pharma, San Diego, California, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Siefker","sequence":"additional","affiliation":[{"name":"aTyr Pharma, San Diego, California, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yvonne","family":"Becker","sequence":"additional","affiliation":[{"name":"Institute of Pathology, University Hospital Bonn, Bonn, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruizhi","family":"Bao","sequence":"additional","affiliation":[{"name":"Institute of Pathology, University Hospital Bonn, Bonn, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erik","family":"Escobedo","sequence":"additional","affiliation":[{"name":"aTyr Pharma, San Diego, California, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Qing","sequence":"additional","affiliation":[{"name":"aTyr Pharma, San Diego, California, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaitlyn","family":"Rauch","sequence":"additional","affiliation":[{"name":"aTyr Pharma, San Diego, California, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luke","family":"Burman","sequence":"additional","affiliation":[{"name":"aTyr Pharma, San Diego, California, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christoph","family":"Burkart","sequence":"additional","affiliation":[{"name":"aTyr Pharma, San Diego, California, 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A novel neuropilin-2 (NRP2) antibody for immunohistochemical staining of patient tissue samples. Eur Respir J, 2022; 60(suppl 66):4531; doi: 10.1183\/13993003.congress-2022.4531","journal-title":"Eur Respir J"},{"key":"e_1_3_6_14_2","doi-asserted-by":"publisher","DOI":"10.1006\/geno.2000.6381"},{"key":"e_1_3_6_15_2","first-page":"27","article-title":"Expression of neuropilin-2 in ovarian cancer","volume":"15","author":"Bednarek W","year":"2011","unstructured":"Bednarek W, , Mazurek-Kociubowska M, , Wertel I. Expression of neuropilin-2 in ovarian cancer. 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