{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T19:21:38Z","timestamp":1777922498578,"version":"3.51.4"},"reference-count":20,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[2023,10,1]],"date-time":"2023-10-01T00:00:00Z","timestamp":1696118400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Monoclonal Antibodies in Immunodiagnosis &amp; Immunotherapy"],"published-print":{"date-parts":[[2023,10]]},"abstract":"<jats:p>\n                    Nattokinase is a protease produced by\n                    <jats:italic toggle=\"yes\">Bacillus subtilis<\/jats:italic>\n                    var.\n                    <jats:italic toggle=\"yes\">natto<\/jats:italic>\n                    that exhibits various beneficial biological effects. Thus, a reliable assay to determine nattokinase levels is needed. In this study, we developed novel mouse monoclonal antibodies (mAbs) that recognize nattokinase, and created a specific and sensitive enzyme-linked immunosorbent assay (ELISA) to measure nattokinase levels. The ELISA was developed using a combination of new mouse antinattokinase mAbs used as capture antibodies coated onto 96-well plates, with a peroxidase-conjugated antibody used for detection. This ELISA enabled detection of nattokinase at 1\u2009ng\/mL. We believe that the novel mAbs developed in this study will be useful in future for elucidating nattokinase function.\n                  <\/jats:p>","DOI":"10.1089\/mab.2023.0012","type":"journal-article","created":{"date-parts":[[2023,10,19]],"date-time":"2023-10-19T11:09:11Z","timestamp":1697713751000},"page":"153-156","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Development of Novel Monoclonal Antibodies Against Nattokinase"],"prefix":"10.1177","volume":"42","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2455-5257","authenticated-orcid":false,"given":"Takashi","family":"Tanikawa","sequence":"first","affiliation":[{"name":"School of Pharmacy, Faculty of Pharmacy and Pharmaceutical Sciences, Josai University, Saitama, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James","family":"Yu","sequence":"additional","affiliation":[{"name":"Contek Life Science Co., Ltd., Taipei, Taiwan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kate","family":"Hsu","sequence":"additional","affiliation":[{"name":"Contek Life Science Co., Ltd., Taipei, Taiwan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shinder","family":"Chen","sequence":"additional","affiliation":[{"name":"Contek Life Science Co., Ltd., Taipei, Taiwan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ayako","family":"Ishii","sequence":"additional","affiliation":[{"name":"CellMark Japan, Tokyo, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takami","family":"Yokogawa","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Faculty of Pharmacy and Pharmaceutical Sciences, Josai University, Saitama, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yutaka","family":"Inoue","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Faculty of Pharmacy and Pharmaceutical Sciences, Josai University, Saitama, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-4741-0320","authenticated-orcid":false,"given":"Masashi","family":"Kitamura","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Faculty of Pharmacy and Pharmaceutical Sciences, Josai University, Saitama, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2023,10,30]]},"reference":[{"key":"e_1_3_5_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biotechadv.2018.12.001"},{"key":"e_1_3_5_3_2","doi-asserted-by":"publisher","DOI":"10.3945\/ajcn.116.137281"},{"key":"e_1_3_5_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01956052"},{"key":"e_1_3_5_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-014-6135-3"},{"key":"e_1_3_5_6_2","doi-asserted-by":"publisher","DOI":"10.1177\/1177271918785130"},{"key":"e_1_3_5_7_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms18030523"},{"key":"e_1_3_5_8_2","doi-asserted-by":"publisher","DOI":"10.3390\/foods11131867"},{"key":"e_1_3_5_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep11601"},{"key":"e_1_3_5_10_2","doi-asserted-by":"publisher","DOI":"10.1291\/hypres.31.1583"},{"key":"e_1_3_5_11_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcvm.2022.964977"},{"key":"e_1_3_5_12_2","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.62.6.25"},{"key":"e_1_3_5_13_2","doi-asserted-by":"publisher","DOI":"10.4014\/jmb.1812.12058"},{"key":"e_1_3_5_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2020.101500"},{"key":"e_1_3_5_15_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12903-021-01914-4"},{"key":"e_1_3_5_16_2","doi-asserted-by":"publisher","DOI":"10.3390\/molecules27175405"},{"key":"e_1_3_5_17_2","doi-asserted-by":"publisher","DOI":"10.1271\/bbb.58.366"},{"key":"e_1_3_5_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.nutres.2009.01.009"},{"key":"e_1_3_5_19_2","doi-asserted-by":"publisher","DOI":"10.1248\/bpb.18.1194"},{"issue":"3","key":"e_1_3_5_20_2","first-page":"16","article-title":"A pilot study on the serum pharmacokinetics of nattokinase in humans following a single, oral, daily dose","volume":"19","author":"Ero M","year":"2013","unstructured":"Ero M, , Ng C, , Mihailovski T, et al. A pilot study on the serum pharmacokinetics of nattokinase in humans following a single, oral, daily dose. Altern Ther Health Med, 2013; 19(3):16\u201319.","journal-title":"Altern Ther Health Med"},{"key":"e_1_3_5_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.alit.2022.11.010"}],"container-title":["Monoclonal Antibodies in Immunodiagnosis &amp; Immunotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1089\/mab.2023.0012","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/mab.2023.0012","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/mab.2023.0012","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T20:22:17Z","timestamp":1777666937000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/full\/10.1089\/mab.2023.0012"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,10]]},"references-count":20,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2023,10]]}},"alternative-id":["10.1089\/mab.2023.0012"],"URL":"https:\/\/doi.org\/10.1089\/mab.2023.0012","relation":{},"ISSN":["2167-9436","2167-9436"],"issn-type":[{"value":"2167-9436","type":"print"},{"value":"2167-9436","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,10]]}}}