{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T21:18:49Z","timestamp":1791839929954,"version":"4.3.4"},"reference-count":0,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[1996,12,1]],"date-time":"1996-12-01T00:00:00Z","timestamp":849398400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[1996,12]]},"abstract":"ABSTRACT<\/jats:title>\n \n In an effort to explore the origin and\/or reservoirs of the genetic determinant(s) of methicillin resistance in\n Staphylococcus aureus<\/jats:italic>\n , we examined over 200 strains representing 13 different species within the genus\n Staphylococcus<\/jats:italic>\n for the presence of the\n mecA<\/jats:italic>\n gene, using a DNA probe internal to this gene prepared from a methicillin-resistant strain of\n S. aureus<\/jats:italic>\n . Occasional\n mecA<\/jats:italic>\n - positive isolates were detected among several staphylococcal species. On the other hand, each one of the 134 isolates of\n Staphylococcus sciuri<\/jats:italic>\n , a species considered taxonomically the most primitive among staphylococci and found primarily on rodents and primitive mammals, gave positive reaction with the DNA probe when tested under conditions of high stringency. About two thirds (99) of these isolates, all of which belonged to\n S. sciuri<\/jats:italic>\n subspecies\n \"sciuri,\"<\/jats:italic>\n as well as 9 of the 11 species\n carnaticum<\/jats:italic>\n isolates, showed only marginal, if any, resistance to methicillin (minimal inhibitory concentration of 0.75\u20136.0 \u03bcg\/ml), while most of the remaining isolates that belonged to the subspecies\n \"rodentius\"<\/jats:italic>\n (13 isolates in all) expressed antibiotic resistance with a heterogeneous phenotype similar to those seen in many methicillin-resistance strains of\n S. aureus<\/jats:italic>\n In\n Sma<\/jats:italic>\n l digests of chromosomal DNA isolated from such \"methicillin-resistant\n S. aureus<\/jats:italic>\n \u2013like\" strains, the\n mecA<\/jats:italic>\n probe hybridized with DNA fragments in the range of 145\u2013180 kb, while in subspecies\n \"sciuri\"<\/jats:italic>\n and\n carnaticum<\/jats:italic>\n isolates the\n mecA<\/jats:italic>\n hybridizing fragment was located in the\n Sma<\/jats:italic>\n I fragment with the highest molecular size (\u2265400 kb). A DNA probe comprising an internal sequence to the regulatory gene\n mecI<\/jats:italic>\n from\n Staphylococcus epidermidis<\/jats:italic>\n identified the presence of sequences with low degree of homology in isolates of the three\n S. sciuri<\/jats:italic>\n subspecies. The\n mecA<\/jats:italic>\n -reacting sequences in these bacteria differed from\n mecA<\/jats:italic>\n of\n S. aureus<\/jats:italic>\n in several respects (e.g., by the absence of a\n Cla<\/jats:italic>\n I restriction site from\n mecA<\/jats:italic>\n of subspecies\n \"sciuri\"<\/jats:italic>\n and\n carnaticum<\/jats:italic>\n , and in some isolates of subspecies\n \"rodentius.\"<\/jats:italic>\n The uniform presence of\n mecA<\/jats:italic>\n in each one of a large number of\n S. sciuri<\/jats:italic>\n strains belonging to distinct ribotypes and macrorestriction patterns and recovered over a 20-year period from a wide variety of animal sources and geographic sites suggests that\n mecA<\/jats:italic>\n may be a native genetic element with an as yet unidentified physiologic function in this staphylococcal species.\n <\/jats:p>","DOI":"10.1089\/mdr.1996.2.377","type":"journal-article","created":{"date-parts":[[2009,1,29]],"date-time":"2009-01-29T19:19:18Z","timestamp":1233256758000},"page":"377-391","source":"Crossref","is-referenced-by-count":152,"title":["Ubiquitous Presence of a\n mecA<\/i>\n Homologue in Natural Isolates of\n Staphylococcus sciuri<\/i>"],"prefix":"10.1177","volume":"2","author":[{"given":"Isabel","family":"Couto","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Herminia","family":"de LENCASTRE","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"Severina","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wesley","family":"Kloos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John A.","family":"Webster","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Romero J.","family":"Hubner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilda Santos","family":"Sanches","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander","family":"Tomasz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[1996,12]]},"container-title":["Microbial Drug Resistance"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/mdr.1996.2.377","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/mdr.1996.2.377","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T18:11:47Z","timestamp":1770747107000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/full\/10.1089\/mdr.1996.2.377"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1996,12]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1996,12]]}},"alternative-id":["10.1089\/mdr.1996.2.377"],"URL":"https:\/\/doi.org\/10.1089\/mdr.1996.2.377","relation":{},"ISSN":["1076-6294","1931-8448"],"issn-type":[{"value":"1076-6294","type":"print"},{"value":"1931-8448","type":"electronic"}],"subject":[],"published":{"date-parts":[[1996,12]]}}}