{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T21:42:20Z","timestamp":1770759740069,"version":"3.50.0"},"reference-count":0,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[1997,12,1]],"date-time":"1997-12-01T00:00:00Z","timestamp":880934400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[1997,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The present work characterizes a 70 kb plasmid of the Indian epidemic isolate\n                    <jats:italic toggle=\"yes\">Shigella dysenteriae<\/jats:italic>\n                    type 1 strain Dt66 in relation to virulence and drug-resistance characters. Curing of the plasmids of Dt66 strain by acriflavine (AF) yielded seven groups of AF-cured derivatives. One group of derivatives containing 5 plasmids (70\u20132.5 kb) as against 6 (120\u20132.5 kb) in the wild type showed resistance to chloramphenicol, pivmecillinam, streptomycin, and tetracycline (Cm\n                    <jats:sup>r<\/jats:sup>\n                    Piv\n                    <jats:sup>r<\/jats:sup>\n                    Sm\n                    <jats:sup>r<\/jats:sup>\n                    Tc\n                    <jats:sup>r<\/jats:sup>\n                    ) but sensitivity for ampicillin and nalidixic acid (AmsNal\n                    <jats:sup>s<\/jats:sup>\n                    ). This derivative when used as donor in conjugation experiments with a plasmidless\n                    <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                    KL318 strain (Am\n                    <jats:sup>s<\/jats:sup>\n                    Cm\n                    <jats:sup>s<\/jats:sup>\n                    Piv\n                    <jats:sup>s<\/jats:sup>\n                    Sm\n                    <jats:sup>r<\/jats:sup>\n                    Tc\n                    <jats:sup>s<\/jats:sup>\n                    Nal\n                    <jats:sup>r<\/jats:sup>\n                    ) as recipient, transferred only its 70 kb plasmid into the recipient with concomitant transfer of the Cm\n                    <jats:sup>r<\/jats:sup>\n                    Piv\n                    <jats:sup>r<\/jats:sup>\n                    phenotype. Both the donor and the transconjugant exhibited a diffuse pattern of adherence and produced keratoconjunctivitis in guinea pigs. Transconjugants contained a 42 kDa lipopolysaccharide band which was absent in recipient. Moreover, both donor and transconjugants showed Congo red binding ability. The results suggest that the 70 kb plasmid of\n                    <jats:italic toggle=\"yes\">S. dysenteriae<\/jats:italic>\n                    Dt66 strain encodes not only Cm\n                    <jats:sup>r<\/jats:sup>\n                    ,Piv\n                    <jats:sup>r<\/jats:sup>\n                    character but also is associated with virulence-related characters such as Congo red binding, LPS-biosynthesis, HeLa cell adherence, and keratoconjunctivitis related to virulence.\n                  <\/jats:p>","DOI":"10.1089\/mdr.1997.3.351","type":"journal-article","created":{"date-parts":[[2009,1,29]],"date-time":"2009-01-29T19:18:17Z","timestamp":1233256697000},"page":"351-357","source":"Crossref","is-referenced-by-count":4,"title":["Involvement of a 70-kb Plasmid of the Epidemic\n                    <i>Shigella dysenteriae<\/i>\n                    Type 1 (Dt66) Strain in Drug-Resistance, Lipopolysaccharide Synthesis, and Virulence"],"prefix":"10.1177","volume":"3","author":[{"given":"S.","family":"Datta","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Pal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Basu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.C.","family":"Banerjee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[1997,12]]},"container-title":["Microbial Drug Resistance"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/mdr.1997.3.351","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/mdr.1997.3.351","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T18:12:51Z","timestamp":1770747171000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/full\/10.1089\/mdr.1997.3.351"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,12]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1997,12]]}},"alternative-id":["10.1089\/mdr.1997.3.351"],"URL":"https:\/\/doi.org\/10.1089\/mdr.1997.3.351","relation":{},"ISSN":["1076-6294","1931-8448"],"issn-type":[{"value":"1076-6294","type":"print"},{"value":"1931-8448","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,12]]}}}