{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T22:16:30Z","timestamp":1770761790576,"version":"3.50.0"},"reference-count":0,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[1998,3,1]],"date-time":"1998-03-01T00:00:00Z","timestamp":888710400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[1998,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    We initiated a survey of the\n                    <jats:italic toggle=\"yes\">Streptococcus pneumoniae<\/jats:italic>\n                    genome by DNA sequence sampling. More than 9,500 random DNA sequences of approximately 500 bases average length were determined. Partial sequences sufficient to identify approximately 95% of the aminoacyl tRNA synthetase genes and ribosomal protein (\n                    <jats:italic toggle=\"yes\">rps<\/jats:italic>\n                    ) genes were found by comparing the database of partial sequences to known sequences from other organisms. Many genes involved in DNA replication, repair, and mutagenesis are present in\n                    <jats:italic toggle=\"yes\">S. pneumoniae<\/jats:italic>\n                    . Genes for the major subunits of RNA polymerase are also present, as are genes for two alternative sigma factors,\n                    <jats:italic toggle=\"yes\">rpoD<\/jats:italic>\n                    and\n                    <jats:italic toggle=\"yes\">rpoN<\/jats:italic>\n                    . Many genes necessary for amino acid or cofactor biosynthesis and aerobic energy metabolism in other bacteria appear to be absent from the\n                    <jats:italic toggle=\"yes\">S. pneumoniae<\/jats:italic>\n                    genome. A number of genes involved in cell wall biosynthesis and septation were identified, including six homologs to different penicillin binding proteins. Interestingly, four genes involved in the addition of D-alanine to lipoteicoic acid in other gram positive bacte[ill]a were found, even though the lipoteicoic acid in\n                    <jats:italic toggle=\"yes\">S. pneumoniae<\/jats:italic>\n                    has not been shown to contain D-alanine. The\n                    <jats:italic toggle=\"yes\">S. pneumoniae<\/jats:italic>\n                    genome contains a number of chaperonin genes similar to those found in other bacteria, but apparently does not contain genes involved in the type III secretion commonly observed in gram negative pathogens. The G+C content of\n                    <jats:italic toggle=\"yes\">S. pneumoniae<\/jats:italic>\n                    genomic DNA is approximately 43 mole percent and the size of the genome is approximately 2.0 Mb as determined by pulsed-field gel electrophoresis. Many of the genes identified by sequence sampling have been physically mapped to the 19 different\n                    <jats:italic toggle=\"yes\">Sma<\/jats:italic>\n                    I fragments derived from the\n                    <jats:italic toggle=\"yes\">S. pneumoniae<\/jats:italic>\n                    genome. The database of random genome sequence tags (GSTs) provides the starting material for determining the complete genome sequence, gene disruption analysis, and comparative genomics to identify novel targets for antibiotic development.\n                  <\/jats:p>","DOI":"10.1089\/mdr.1998.4.1","type":"journal-article","created":{"date-parts":[[2009,1,29]],"date-time":"2009-01-29T12:25:45Z","timestamp":1233231945000},"page":"1-9","source":"Crossref","is-referenced-by-count":19,"title":["DNA Sequence Sampling of the\n                    <i>Streptococcus pneumoniae<\/i>\n                    Genome to Identify Novel Targets for Antibiotic Development"],"prefix":"10.1177","volume":"4","author":[{"given":"R.H.","family":"Baltz","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F.H.","family":"Norris","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"Matsushima","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.S.","family":"DeHOFF","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"Rockey","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.","family":"Porter","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Burgett","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Peery","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Hoskins","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"Braverman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I.","family":"Jenkins","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"Solenberg","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Young","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.A.","family":"McHENNEY","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.L.","family":"Skatrud","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"Jr.","given":"P.R.","family":"Rosteck","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[1998,3]]},"container-title":["Microbial Drug Resistance"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/mdr.1998.4.1","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/mdr.1998.4.1","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T18:13:25Z","timestamp":1770747205000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/full\/10.1089\/mdr.1998.4.1"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998,3]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1998,3]]}},"alternative-id":["10.1089\/mdr.1998.4.1"],"URL":"https:\/\/doi.org\/10.1089\/mdr.1998.4.1","relation":{},"ISSN":["1076-6294","1931-8448"],"issn-type":[{"value":"1076-6294","type":"print"},{"value":"1931-8448","type":"electronic"}],"subject":[],"published":{"date-parts":[[1998,3]]}}}