{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T23:06:47Z","timestamp":1770764807838,"version":"3.50.0"},"reference-count":0,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2000,12,1]],"date-time":"2000-12-01T00:00:00Z","timestamp":975628800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2000,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    A multitude of extended spectrum \u03b2-lactamases (ESBLs) have evolved in response to the use of late generation cephalosporins. In those hospitals where\n                    <jats:italic toggle=\"yes\">Klebsiella pneumoniae<\/jats:italic>\n                    and other bacteria possessing these enzymes flourish, many interventions have been applied to reduce this trend. We instituted a policy of class restriction of cephalosporins in our hospital in 1996 that led to a 44% reduction in ceftazidime-resistant\n                    <jats:italic toggle=\"yes\">K. pneumoniae<\/jats:italic>\n                    hospital-wide and an 87% decrease in the surgical intensive care unit. Another interesting outcome of this strategy was the identification of multiresistant\n                    <jats:italic toggle=\"yes\">K. pneumoniae<\/jats:italic>\n                    , which was now susceptible to ceftazidime. Characterization of these novel isolates demonstrated that the TEM-26 enzyme, which was responsible for ceftazidime resistance in our earlier described outbreak, was lacking in most of the isolates examined. Among the remaining ceftazidime-resistant\n                    <jats:italic toggle=\"yes\">K. pneumoniae<\/jats:italic>\n                    , TEM-26 was also absent, and new enzymes that hydrolyze ceftazidime were detected. Loss of ceftazidime-hydrolyzing \u03b2-lactamases was observed after\n                    <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                    passage of ceftazidime-resistant\n                    <jats:italic toggle=\"yes\">K. pneumoniae<\/jats:italic>\n                    on antibiotic-free media. These findings suggest that class restriction of cephalosporins may increase susceptibility among extended-spectrum \u03b2-lactamase-producing pathogens.\n                  <\/jats:p>","DOI":"10.1089\/mdr.2000.6.297","type":"journal-article","created":{"date-parts":[[2009,1,28]],"date-time":"2009-01-28T15:35:48Z","timestamp":1233156948000},"page":"297-303","source":"Crossref","is-referenced-by-count":9,"title":["Detection of Multiresistant Ceftazidime-Susceptible\n                    <i>Klebsiella pneumoniae<\/i>\n                    Isolates Lacking TEM-26 after Class Restriction of Cephalosporins"],"prefix":"10.1177","volume":"6","author":[{"given":"Carl","family":"Urban","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Noriel","family":"Mariano","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naim","family":"Rahman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anne Marie","family":"Queenan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Deborah","family":"Montenegro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karen","family":"Bush","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James J.","family":"Rahal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2000,12]]},"container-title":["Microbial Drug Resistance"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/mdr.2000.6.297","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/mdr.2000.6.297","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T18:13:50Z","timestamp":1770747230000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/full\/10.1089\/mdr.2000.6.297"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,12]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2000,12]]}},"alternative-id":["10.1089\/mdr.2000.6.297"],"URL":"https:\/\/doi.org\/10.1089\/mdr.2000.6.297","relation":{},"ISSN":["1076-6294","1931-8448"],"issn-type":[{"value":"1076-6294","type":"print"},{"value":"1931-8448","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000,12]]}}}