{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T19:22:22Z","timestamp":1777922542264,"version":"3.51.4"},"reference-count":0,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2006,3,1]],"date-time":"2006-03-01T00:00:00Z","timestamp":1141171200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2006,3]]},"abstract":"\n Klebsiella pneumoniae<\/jats:italic>\n causing primary liver abscess (PLA) is emerging. This study identified the\n \u03b2<\/jats:italic>\n -lactamases genes of\n K. pneumoniae<\/jats:italic>\n isolates in Taiwan. The susceptibilities of\n \u03b2<\/jats:italic>\n -lactam antibiotics of 30\n K. pneumoniae<\/jats:italic>\n strains associated with primary liver abscess and 30 noninvasive strains were analyzed. The\n \u03b2<\/jats:italic>\n -lactamase genes of randomly selected 24 strains from community-acquired infection and 7 extended-spectrum\n \u03b2<\/jats:italic>\n -lactamases (ESBL) strains were identified by PCR and DNA sequencing. Protein expression and the ESBL phenotype of\n \u03b2<\/jats:italic>\n -lactamase were determined. All 60 strains were ampicillin resistant and cefotaxime susceptible, whereas no strain was ESBL producing. In the 24 selected strains,\n shv-1a<\/jats:italic>\n was found in 14,\n shv-1<\/jats:italic>\n in 7;\n shv-26<\/jats:italic>\n ,\n shv-27<\/jats:italic>\n , and\n shv-41<\/jats:italic>\n were detected in one. However, all of these 24 strains had the\n tem-116<\/jats:italic>\n gene. In 7 ESBL-producing\n K. pneumoniae<\/jats:italic>\n strains,\n shv-5a<\/jats:italic>\n was found in 5, whereas\n shv-5<\/jats:italic>\n and\n ctx-m-9<\/jats:italic>\n group were detected in 1 strain. Two previously reported ESBL genes,\n shv-27<\/jats:italic>\n and\n tem-116<\/jats:italic>\n , as well as a suspected ESBL gene,\n shv-41<\/jats:italic>\n , were found in non-ESBL-producing strains. Transformation of these genes conferred ampicillin resistance but not the ESBL-producing phenotype in\n Escherichia coli<\/jats:italic>\n .\n \u03b2<\/jats:italic>\n -Lactamase protein expression of these strains was further confirmed by western blotting. In conclusion, ESBL is rare in community-acquired\n K. pneumoniae<\/jats:italic>\n infection\nand is not associated with PLA in Taiwan. The\n shv-5a<\/jats:italic>\n ,\n shv-5<\/jats:italic>\n , and\n ctx-m-9<\/jats:italic>\n groups are present in ESBL-producing strains in Taiwan, but\n shv-27<\/jats:italic>\n ,\n shv-41<\/jats:italic>\n , and\n tem-116<\/jats:italic>\n are not ESBL genes.\n <\/jats:p>","DOI":"10.1089\/mdr.2006.12.12","type":"journal-article","created":{"date-parts":[[2006,4,3]],"date-time":"2006-04-03T10:49:08Z","timestamp":1144061348000},"page":"12-15","source":"Crossref","is-referenced-by-count":27,"title":["Extended-Spectrum\n \u03b2<\/i>\n -Lactamase Genes of\n Klebsiella pneumoniae<\/i>\n Strains in Taiwan: Recharacterization of\n shv-27<\/i>\n ,\n shv-41<\/i>\n , and\n tem-116<\/i>"],"prefix":"10.1177","volume":"12","author":[{"given":"Tzu-Lung","family":"Lin","sequence":"first","affiliation":[{"name":"Department of Microbiology, National Taiwan University College of Medicine, Taipei, Taiwan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Su-Ing","family":"Tang","sequence":"additional","affiliation":[{"name":"Department of Microbiology, National Taiwan University College of Medicine, Taipei, Taiwan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chi-Tai","family":"Fang","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, National Taiwan University Hospital, Taipei, Taiwan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Po-Ren","family":"Hsueh","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, National Taiwan University Hospital, Taipei, Taiwan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shan-Chwen","family":"Chang","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, National Taiwan University Hospital, Taipei, Taiwan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jin-Town","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Microbiology, National Taiwan University College of Medicine, Taipei, Taiwan."},{"name":"Department of Internal Medicine, National Taiwan University Hospital, Taipei, Taiwan."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2006,3]]},"container-title":["Microbial Drug Resistance"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/mdr.2006.12.12","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/mdr.2006.12.12","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T20:23:24Z","timestamp":1777667004000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/full\/10.1089\/mdr.2006.12.12"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,3]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2006,3]]}},"alternative-id":["10.1089\/mdr.2006.12.12"],"URL":"https:\/\/doi.org\/10.1089\/mdr.2006.12.12","relation":{},"ISSN":["1076-6294","1931-8448"],"issn-type":[{"value":"1076-6294","type":"print"},{"value":"1931-8448","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,3]]}}}