{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T07:12:40Z","timestamp":1790233960545,"version":"4.1.0"},"reference-count":0,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2007,9,1]],"date-time":"2007-09-01T00:00:00Z","timestamp":1188604800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2007,9]]},"abstract":"\n Pseudomonas aeruginosa<\/jats:italic>\n isolates carrying IMP- or VIM-type metallo-\n \u03b2<\/jats:italic>\n -lactamase (MBL) have been increasingly reported in hospitals worldwide. One hundred\n P. aeruginosa<\/jats:italic>\n clinical isolates from unrelated inpatients hospitalized at a Turkish university hospital were screened for the presence of\n bla<\/jats:italic>\n IMP<\/jats:sub>\n and\n bla<\/jats:italic>\n VIM<\/jats:sub>\n genes by polymerase chain reaction (PCR). One (1%) isolate was found to carry a VIM-type MBL gene, whereas nine (9%) carried an IMP-1 MBL gene carried on a cassette inserted into a class 1 integron. Only four of the IMP producers were detected as MBL producers according to E-test MBL. Minimum inhibitory concentrations (MICs) of imipenem for the IMP-1 and VIM-type MBL-producers were highly variable (MIC values, 8\u2013128\n \u03bc<\/jats:italic>\n g\/ml). Imipenem resistance was not plasmid-mediated according to the transformation assays. Piperacillin\/tazobactam was the only effective drug in antimicrobial susceptibility testing. No aztreonam-resistant IMP and VIM producers were detected to produce an extended-spectrum\n \u03b2<\/jats:italic>\n -lactamase (ESBL). Three class 1 integrons of approximately 2,300 bp, 1,800 bp, and 1,500 bp in size were detected in each of the nine IMP-positive isolates. Sequencing revealed three novel gene cassette arrays,\n aac(3)<\/jats:italic>\n -\n 1c<\/jats:italic>\n \u2013\n cmlA5<\/jats:italic>\n ,\n bla<\/jats:italic>\n IMP-1<\/jats:sub>\n \u2013\n aadA7<\/jats:italic>\n -like, and\n aacA7<\/jats:italic>\n \u2013\n smr-2<\/jats:italic>\n \u2013\n orfD<\/jats:italic>\n . Enterobacterial repetitive intergenic consensus PCR (ERIC-PCR) indicated that a clonal spread of IMP-1-producers had occurred in this hospital.\n <\/jats:p>","DOI":"10.1089\/mdr.2007.748","type":"journal-article","created":{"date-parts":[[2007,10,21]],"date-time":"2007-10-21T11:12:25Z","timestamp":1192965145000},"page":"191-198","source":"Crossref","is-referenced-by-count":31,"title":["Molecular Epidemiology of Clinical\n Pseudomonas aeruginosa<\/i>\n Isolates Carrying IMP-1 Metallo-\n \u03b2<\/i>\n -Lactamase Gene in a University Hospital in Turkey"],"prefix":"10.1177","volume":"13","author":[{"given":"Osman Birol","family":"Ozgumus","sequence":"first","affiliation":[{"name":"Department of Biology, Rize Faculty of Arts & Sciences, Karadeniz Technical University, 53100 Rize, Turkey."},{"name":"Department of Biology, Faculty of Arts & Sciences, Rize University, 53100 Rize, Turkey."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rahmet","family":"Caylan","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases and Clinical Microbiology, Faculty of Medicine, Karadeniz Technical University, 61080 Trabzon, Turkey."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilknur","family":"Tosun","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Clinical Microbiology, Faculty of Medicine, Karadeniz Technical University, 61080 Trabzon, Turkey."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cemal","family":"Sandalli","sequence":"additional","affiliation":[{"name":"Department of Biology, Rize Faculty of Arts & Sciences, Karadeniz Technical University, 53100 Rize, Turkey."},{"name":"Department of Biology, Faculty of Arts & Sciences, Rize University, 53100 Rize, Turkey."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kemalettin","family":"Aydin","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases and Clinical Microbiology, Faculty of Medicine, Karadeniz Technical University, 61080 Trabzon, Turkey."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iftihar","family":"Koksal","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases and Clinical Microbiology, Faculty of Medicine, Karadeniz Technical University, 61080 Trabzon, Turkey."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2007,9]]},"container-title":["Microbial Drug Resistance"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/mdr.2007.748","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/mdr.2007.748","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T20:24:13Z","timestamp":1777667053000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/full\/10.1089\/mdr.2007.748"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,9]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2007,9]]}},"alternative-id":["10.1089\/mdr.2007.748"],"URL":"https:\/\/doi.org\/10.1089\/mdr.2007.748","relation":{},"ISSN":["1076-6294","1931-8448"],"issn-type":[{"value":"1076-6294","type":"print"},{"value":"1931-8448","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,9]]}}}